Androgens augment proximal tubule transport

Albert Quan, Sumana Chakravarty, Jiankang Chen, Jian Chun Chen, Samer Loleh, Neel Saini, Ray C. Harris, Jorge Capdevila, Raymond Quigley

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

The proximal tubule contains an autonomous renin-angiotensin system that regulates transport independently of circulating angiotensin II. Androgens are known to increase expression of angiotensinogen, but the effect of androgens on proximal tubule transport is unknown. In this in vivo microperfusion study, we examined the effect of androgens on proximal tubule transport. The volume reabsorptive rate in Sprague-Dawiey rats given dihydrotestosterone (DHT) injections was significantly higher than in control rats given vehicle injections (4.57 ± 0.31 vs. 3.31 ± 0.23 nl·min -1·mm-1, P < 0.01). Luminally perfusing with either enalaprilat (10-4 M) to inhibit production of angiotensin II or losartan (10-8 M) to block the angiotensin receptor decreased the proximal tubule volume reabsorptive rate in DHT-treated rats to a significantly greater degree than in control vehicle-injected rats. The renal expression of angiotensinogen was shown to be higher in the DHT-treated animals, using Northern blot analysis. The expression of angiotensin receptors, determined by specific binding of angiotensin II, was not different in the two groups of animals. Brush-border membrane protein abundance of the Na/H exchanger, a membrane transport protein under angiotensin II regulation, was also higher in DHT-treated rats vs. control rats. Rats that received DHT had higher blood pressures than the control rats but had no change in their glomerular filtration rate. In addition, serum angiotensin II levels were lower in DHT-treated vs. control rats. These results suggest that androgens may directly upregulate the proximal tubule renin-angiotensin system, increase the volume reabsorptive rate, and thereby increase extracellular volume and blood pressure and secondarily decrease serum angiotensin II levels.

Original languageEnglish (US)
Pages (from-to)F452-F459
JournalAmerican Journal of Physiology - Renal Physiology
Volume287
Issue number3 56-3
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

Fingerprint

Androgens
Dihydrotestosterone
Angiotensin II
Angiotensinogen
Angiotensin Receptors
Renin-Angiotensin System
Enalaprilat
Sodium-Hydrogen Antiporter
Injections
Losartan
Membrane Transport Proteins
Microvilli
Serum
Glomerular Filtration Rate
Northern Blotting
Membrane Proteins
Up-Regulation
Blood Pressure
Hypertension
Kidney

Keywords

  • Dihydrotestosterone
  • Microperfusion
  • Renin-angiotensin
  • Testosterone

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Quan, A., Chakravarty, S., Chen, J., Chen, J. C., Loleh, S., Saini, N., ... Quigley, R. (2004). Androgens augment proximal tubule transport. American Journal of Physiology - Renal Physiology, 287(3 56-3), F452-F459. https://doi.org/10.1152/ajprenal.00188.2003

Androgens augment proximal tubule transport. / Quan, Albert; Chakravarty, Sumana; Chen, Jiankang; Chen, Jian Chun; Loleh, Samer; Saini, Neel; Harris, Ray C.; Capdevila, Jorge; Quigley, Raymond.

In: American Journal of Physiology - Renal Physiology, Vol. 287, No. 3 56-3, 01.09.2004, p. F452-F459.

Research output: Contribution to journalArticle

Quan, A, Chakravarty, S, Chen, J, Chen, JC, Loleh, S, Saini, N, Harris, RC, Capdevila, J & Quigley, R 2004, 'Androgens augment proximal tubule transport', American Journal of Physiology - Renal Physiology, vol. 287, no. 3 56-3, pp. F452-F459. https://doi.org/10.1152/ajprenal.00188.2003
Quan, Albert ; Chakravarty, Sumana ; Chen, Jiankang ; Chen, Jian Chun ; Loleh, Samer ; Saini, Neel ; Harris, Ray C. ; Capdevila, Jorge ; Quigley, Raymond. / Androgens augment proximal tubule transport. In: American Journal of Physiology - Renal Physiology. 2004 ; Vol. 287, No. 3 56-3. pp. F452-F459.
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