AngII induces transient phospholipase D activity in the H295R glomerulosa cell model

Xiangjian Zheng, Wendy B Bollag

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In this report we demonstrate that in human adrenocortical carcinoma NCI H295R cells, a model for adrenal glomerulosa cells, PLD was activated both by AngII and protein kinase C (PKC)-activating phorbol 12-myristate 13-acetate (PMA). However, while PMA triggered sustained PLD activation, AngII induced transient PLD activation, in contrast to results in bovine glomerulosa cells in primary culture. Despite the transient effect of AngII on PLD activity, PLD-derived lipid signals were required for maximal AngII-elicited aldosterone secretion. AngII-induced PLD activation was inhibited by PKC inhibitors, but not by tyrosine kinase or calcium/calmodulin-dependent kinase inhibitors or a calmodulin antagonist. Both AngII- and PMA-stimulated PLD activity was enhanced by phosphoinositide 3-kinase (PI3K) inhibitors. Akt, a downstream protein kinase activated by the products of PI3K, was constitutively active in H295R cells, and this activity was blocked by PI3K inhibitors. These results suggested that in H295R adrenocortical carcinoma cells, AngII-induced PLD activation was promoted by PKC and inhibited by the constitutively active PI3K pathway.

Original languageEnglish (US)
Pages (from-to)113-122
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume206
Issue number1-2
DOIs
StatePublished - Aug 29 2003

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Phospholipase D
1-Phosphatidylinositol 4-Kinase
Phosphatidylinositols
Phosphotransferases
Chemical activation
Protein Kinase C
Adrenocortical Carcinoma
Acetates
Calmodulin
Protein-Tyrosine Kinases
Calcium-Calmodulin-Dependent Protein Kinase Kinase
Zona Glomerulosa
Primary Cell Culture
1-dodecylpyridoxal
Protein C Inhibitor
Protein Kinase Inhibitors
Aldosterone
Protein Kinases
Cells
Calcium

Keywords

  • Adrenocortical carcinoma
  • Aldosterone
  • NCI H295R cells
  • Phosphatidic acid
  • Phosphoinositide 3-kinase
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

AngII induces transient phospholipase D activity in the H295R glomerulosa cell model. / Zheng, Xiangjian; Bollag, Wendy B.

In: Molecular and Cellular Endocrinology, Vol. 206, No. 1-2, 29.08.2003, p. 113-122.

Research output: Contribution to journalArticle

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