Angiotensin II activates the GFAT promoter in mesangial cells

Leighton R James, Alistair Ingram, Hao Ly, Kerri Thai, Lu Cai, James W. Scholey

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Expression of glutamine: Fructose-6-phosphate amidotransferase (GFAT), the rate-limiting enzyme for glucose entry into the hexosamine pathway, is transcriptionally regulated. Immunohistochemical studies of human kidney biopsies demonstrate increased GFAT expression in diabetic glomeruli, but the mechanism responsible for this overexpression is unknown. Given the role of ANG II in diabetic kidney disease, we chose to study the effect of ANG II on GFAT promoter activity in mesangial cells (MC). Exposure of MC to ANG II (10-7 M) increased GFAT promoter activity (2.5-fold), mRNA (3-fold), and protein (1.6-fold). ANG II-mediated GFAT promoter activation was inhibited by the ANG II type I receptor antagonist candesartan (10-8 M) but was unaffected by the ANG II type II receptor antagonist PD-123319 (10-8 M). The intracellular calcium chelator 1,2-bis(2-aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (10-6 M), protein kinase C (PKC) inhibitors bisindoylmaleimide-4 (10-6 M) and calphostin C (10-7 M), protein tyrosine kinase (PTK) inhibitor genistein (10-4 M), Src family kinase inhibitor PP2 (2.5 × 10-7 M), p42/44 mitogen-activated protein kinase (MAPK) inhibitor PD-98059 (10-5 M), and the epidermal growth factor (EGF) inhibitor AG-1478 all attenuated GFAT promoter activation by ANG II. We conclude that the GFAT promoter is activated by ANG II via the AT1 receptor. Promoter activation is calcium dependent and PKC dependent but also involves PTK signaling pathways including Src, the EGF receptor, and p42/44 MAPK.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume281
Issue number1 50-1
StatePublished - Oct 10 2001
Externally publishedYes

Fingerprint

Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
Mesangial Cells
Angiotensin II
Protein Kinase Inhibitors
Mitogen-Activated Protein Kinase 1
Protein-Tyrosine Kinases
Protein Kinase C
Hexosamines
Protein C Inhibitor
Genistein
Diabetic Nephropathies
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Kidney
Biopsy
Glucose
Messenger RNA
Acids

Keywords

  • Angiotensin II
  • Glomerulus
  • Glutamine:Fructose-6-phosphate amidotransferase
  • Mesangial cells
  • Signaling

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

James, L. R., Ingram, A., Ly, H., Thai, K., Cai, L., & Scholey, J. W. (2001). Angiotensin II activates the GFAT promoter in mesangial cells. American Journal of Physiology - Renal Physiology, 281(1 50-1).

Angiotensin II activates the GFAT promoter in mesangial cells. / James, Leighton R; Ingram, Alistair; Ly, Hao; Thai, Kerri; Cai, Lu; Scholey, James W.

In: American Journal of Physiology - Renal Physiology, Vol. 281, No. 1 50-1, 10.10.2001.

Research output: Contribution to journalArticle

James, LR, Ingram, A, Ly, H, Thai, K, Cai, L & Scholey, JW 2001, 'Angiotensin II activates the GFAT promoter in mesangial cells', American Journal of Physiology - Renal Physiology, vol. 281, no. 1 50-1.
James, Leighton R ; Ingram, Alistair ; Ly, Hao ; Thai, Kerri ; Cai, Lu ; Scholey, James W. / Angiotensin II activates the GFAT promoter in mesangial cells. In: American Journal of Physiology - Renal Physiology. 2001 ; Vol. 281, No. 1 50-1.
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