Angiotensin II controls p21ras activity via pp60c-src

Bernhard Schieffer, William G. Paxton, Qing Chai, Mario B Marrero, Kenneth E. Bernstein

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Angiotensin II is the major effector peptide of the renin-angiotensin system, and it exerts its physiologic functions via a G protein-coupled cell surface receptor called AT1. We found that in rat aortic smooth muscle cells, angiotensin II stimulated the formation of Ras-GTP, Ras-Raf-1 complex formation, and the tyrosine phosphorylation of two important Ras GTPase-activating proteins (GAPs), p120 Ras-GAP and p190 Rho-GAP. Electroporation of anti-pp60c-src antibody into cultured, adherent smooth muscle cells blocked the angiotensin II stimulation of Ras-GAP and Rho-GAP tyrosine phosphorylation. In contrast electroporation of antibodies against c-Yes or c-Fyn had no effect. Anti-pp60c-src antibody also blocked angiotensin II-stimulated Ras activation and Ras-Raf-1 complex formation. These data strongly suggest that a G protein-coupled receptor such as the AT1 receptor can activate the Ras protein cascade via the tyrosine kinase pp60c-src.

Original languageEnglish (US)
Pages (from-to)10329-10333
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number17
DOIs
StatePublished - Apr 26 1996

Fingerprint

ras GTPase-Activating Proteins
Angiotensin II
Phosphorylation
Electroporation
Smooth Muscle Myocytes
Tyrosine
p120 GTPase Activating Protein
Muscle
Antibodies
Cells
ras Proteins
src-Family Kinases
Angiotensins
Cell Surface Receptors
Renin-Angiotensin System
G-Protein-Coupled Receptors
Guanosine Triphosphate
GTP-Binding Proteins
Renin
Rats

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Schieffer, B., Paxton, W. G., Chai, Q., Marrero, M. B., & Bernstein, K. E. (1996). Angiotensin II controls p21ras activity via pp60c-src Journal of Biological Chemistry, 271(17), 10329-10333. https://doi.org/10.1074/jbc.271.17.10329

Angiotensin II controls p21ras activity via pp60c-src . / Schieffer, Bernhard; Paxton, William G.; Chai, Qing; Marrero, Mario B; Bernstein, Kenneth E.

In: Journal of Biological Chemistry, Vol. 271, No. 17, 26.04.1996, p. 10329-10333.

Research output: Contribution to journalArticle

Schieffer, B, Paxton, WG, Chai, Q, Marrero, MB & Bernstein, KE 1996, 'Angiotensin II controls p21ras activity via pp60c-src ', Journal of Biological Chemistry, vol. 271, no. 17, pp. 10329-10333. https://doi.org/10.1074/jbc.271.17.10329
Schieffer B, Paxton WG, Chai Q, Marrero MB, Bernstein KE. Angiotensin II controls p21ras activity via pp60c-src Journal of Biological Chemistry. 1996 Apr 26;271(17):10329-10333. https://doi.org/10.1074/jbc.271.17.10329
Schieffer, Bernhard ; Paxton, William G. ; Chai, Qing ; Marrero, Mario B ; Bernstein, Kenneth E. / Angiotensin II controls p21ras activity via pp60c-src In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 17. pp. 10329-10333.
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