Angiotensin II hypertension is attenuated in interleukin-6 knockout mice

Dexter L. Lee, LaShon Sturgis, Hicham Labazi, James B. Osborne, Cassandra Fleming, Jennifer S. Pollock, Marlina Manhiani, John D. Imig, Michael W Brands

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Plasma levels of IL-6 correlate with high blood pressure under many circumstances, and ANG II has been shown to stimulate IL-6 production from various cell types. This study tested the role of IL-6 in mediating the hypertension caused by high-dose ANG II and a high-salt diet. Male C57BL6 and IL-6 knockout (IL-6 KO) mice were implanted with biotelemetry devices and placed in metabolic cages to measure mean arterial pressure (MAP), heart rate (HR), sodium balance, and urinary albumin excretion. Baseline MAP during the control period averaged 114 ± 1 and 109 ± 1 mmHg for wild-type (WT) and IL-6 KO mice, respectively, and did not change significantly when the mice were placed on a high-salt diet (HS; 4% NaCl). ANG II (90 ng/min sc) caused a rapid increase in MAP in both groups, to 141 ± 9 and 141 ± 4 in WT and KO mice, respectively, on day 2. MAP plateaued at this level in KO mice (134 ± 2 mmHg on day 14 of ANG II) but began to increase further in WT mice by day 4, reaching an average of 160 ± 4 mmHg from days 10 to 14 of ANG II. Urinary albumin excretion on day 4 of ANG II was not different between groups (9.18 ± 4.34 and 8.53 ± 2.85 μg/2 days for WT and KO mice). By day 14, albumin excretion was nearly fourfold greater in WT mice, but MAP dropped rapidly back to control levels in both groups when the ANG II was stopped after 14 days. Thus the ∼30 mmHg greater ANG II hypertension in the WT mice suggests that IL-6 contributes significantly to ANG II-salt hypertension. In addition, the early separation in MAP, the albumin excretion data, and the rapid, post-ANG II recovery of MAP suggest an IL-6-dependent mechanism that is independent of renal injury.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume290
Issue number3
DOIs
StatePublished - Mar 1 2006

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Knockout Mice
Angiotensin II
Interleukin-6
Arterial Pressure
Hypertension
Albumins
Salts
Diet
Heart Rate
Sodium
Kidney
Equipment and Supplies
Wounds and Injuries

Keywords

  • High-salt diet
  • Mean arterial pressure

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Angiotensin II hypertension is attenuated in interleukin-6 knockout mice. / Lee, Dexter L.; Sturgis, LaShon; Labazi, Hicham; Osborne, James B.; Fleming, Cassandra; Pollock, Jennifer S.; Manhiani, Marlina; Imig, John D.; Brands, Michael W.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 290, No. 3, 01.03.2006.

Research output: Contribution to journalArticle

Lee, Dexter L. ; Sturgis, LaShon ; Labazi, Hicham ; Osborne, James B. ; Fleming, Cassandra ; Pollock, Jennifer S. ; Manhiani, Marlina ; Imig, John D. ; Brands, Michael W. / Angiotensin II hypertension is attenuated in interleukin-6 knockout mice. In: American Journal of Physiology - Heart and Circulatory Physiology. 2006 ; Vol. 290, No. 3.
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AB - Plasma levels of IL-6 correlate with high blood pressure under many circumstances, and ANG II has been shown to stimulate IL-6 production from various cell types. This study tested the role of IL-6 in mediating the hypertension caused by high-dose ANG II and a high-salt diet. Male C57BL6 and IL-6 knockout (IL-6 KO) mice were implanted with biotelemetry devices and placed in metabolic cages to measure mean arterial pressure (MAP), heart rate (HR), sodium balance, and urinary albumin excretion. Baseline MAP during the control period averaged 114 ± 1 and 109 ± 1 mmHg for wild-type (WT) and IL-6 KO mice, respectively, and did not change significantly when the mice were placed on a high-salt diet (HS; 4% NaCl). ANG II (90 ng/min sc) caused a rapid increase in MAP in both groups, to 141 ± 9 and 141 ± 4 in WT and KO mice, respectively, on day 2. MAP plateaued at this level in KO mice (134 ± 2 mmHg on day 14 of ANG II) but began to increase further in WT mice by day 4, reaching an average of 160 ± 4 mmHg from days 10 to 14 of ANG II. Urinary albumin excretion on day 4 of ANG II was not different between groups (9.18 ± 4.34 and 8.53 ± 2.85 μg/2 days for WT and KO mice). By day 14, albumin excretion was nearly fourfold greater in WT mice, but MAP dropped rapidly back to control levels in both groups when the ANG II was stopped after 14 days. Thus the ∼30 mmHg greater ANG II hypertension in the WT mice suggests that IL-6 contributes significantly to ANG II-salt hypertension. In addition, the early separation in MAP, the albumin excretion data, and the rapid, post-ANG II recovery of MAP suggest an IL-6-dependent mechanism that is independent of renal injury.

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