Angiotensin II priming of aldosterone secretion with agents that enhance Ca2+ influx

Soraya Betancourt-Calle, RaShawn Mann-Blakeney, Carlos M Isales, Roberto A. Calle, Wendy B Bollag

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have previously shown that angiotensin II (AngII) is able to prime, or sensitize, the secretory response of cultured bovine adrenal glomerulosa cells to the Ca2+ channel agonist, BAY K8644. We examined the ability of AngII to prime glomerulosa cells to an elevated extracellular K+ level, a physiological agonist that also triggers Ca2+ influx. K+ (9 mM) elicited enhanced secretion in AngII-primed cells compared to those with no prior exposure to the hormone, suggesting that AngII can sensitize glomerulosa cells to respond to increases in extracellular K+. The potential involvement of protein kinase C (PKC) in priming was investigated by determining whether enhanced Ca2+ influx could maintain the AngII-induced phosphorylation of the endogenous PKC substrate, myristoylated, alanine-rich C kinase substrate (MARCKS). Incubation with the AngII antagonist, saralasin, for 30 min following an AngII exposure reduced the AngII-induced increase in MARCKS phosphorylation. 100 nM BAY K8644 together with saralasin was unable to maintain AngII-stimulated MARCKS phosphorylation. On the other hand, phosphorylation of the steroidogenic acute regulatory (StAR) protein was sustained with saralasin exposure, both in the presence and absence of BAY K8644. This observation suggests that persistent StAR phosphorylation/activation may play a role in priming.

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume177
Issue number1-2
DOIs
StatePublished - May 25 2001

Fingerprint

Aldosterone
Angiotensin II
Phosphorylation
Saralasin
Protein Kinase C
Zona Glomerulosa
Chemical activation
Hormones
Substrates

Keywords

  • Adrenal glomerulosa
  • Angiotensin II
  • MARCKS
  • Phosphorylation
  • Protein kinase C
  • StAR

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Angiotensin II priming of aldosterone secretion with agents that enhance Ca2+ influx. / Betancourt-Calle, Soraya; Mann-Blakeney, RaShawn; Isales, Carlos M; Calle, Roberto A.; Bollag, Wendy B.

In: Molecular and Cellular Endocrinology, Vol. 177, No. 1-2, 25.05.2001, p. 61-70.

Research output: Contribution to journalArticle

Betancourt-Calle, Soraya ; Mann-Blakeney, RaShawn ; Isales, Carlos M ; Calle, Roberto A. ; Bollag, Wendy B. / Angiotensin II priming of aldosterone secretion with agents that enhance Ca2+ influx. In: Molecular and Cellular Endocrinology. 2001 ; Vol. 177, No. 1-2. pp. 61-70.
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