Angiotensin II receptor binding and actions in NG108-15 cells.

R. C. Speth, L. Mei, H. I. Yamamura

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

NG108-15 cells were grown in culture to confluency and membranes were prepared from the cells to test for the presence of angiotensin II (Ang II) receptors using 125I-sarcosine1, isoleucine8 angiotensin II (125I-SI Ang II). These radioligand binding studies indicated a single class of binding sites with high affinity (KD = 221 +/- 54 pM) that were saturable (Bmax = 27.2 +/- 1.6 fmol/mg protein) and showed characteristic specificity (SI Ang II greater than Ang II greater than Sar1Thr8 Ang II greater than Ang III greater than Ang I greater than des Phe8 Ang II greater than des Asp1, Arg2, Val3 pentapeptide Ang II). To see if these putative receptor binding sites were associated with a functional response, the effect of Ang II on phosphatidylinositide (PtdIns) hydrolysis, expressed as inositol monophosphate (IP1) formation in intact NG108-15 cells preloaded with 3H-myoinositol, was determined. Ang II inhibited IP1 formation up to 35% below the basal rate in a dose related manner. The inhibition of PtdIns hydrolysis was prevented by pre-exposure of the cells to SI Ang II, an Ang II receptor antagonist. These results indicate that undifferentiated NG108-15 cells possess Ang II receptors that mediate a reduction in PtdIns hydrolysis.

Original languageEnglish (US)
Pages (from-to)232-239
Number of pages8
JournalPeptide research
Volume2
Issue number3
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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    Speth, R. C., Mei, L., & Yamamura, H. I. (1989). Angiotensin II receptor binding and actions in NG108-15 cells. Peptide research, 2(3), 232-239.