Angiotensin ii stimulates the tyrosine phosphorylation and activation of irs-i and ftp-id in rat aortic smooth muscle cells

Mano B. Marrero, Elisabcch Schicflcr, Bernhard Schieffer

Research output: Contribution to journalArticle

Abstract

Angiotensin II (Ang II) and the insulin like growth factor I (IGF I) mediate similar intracctlular signaling events through cell surface receptors with different structural and biochemical properties. For example, in rat aortic smooth muscle (RASM) cells, we find that IGF I induces the tyrosinc phosphorylation of the insulin receptor substrate 1 (IRS-1) (a src-homology 2 (SH2) domain containing docking protein) and the soluble SH2 binding tyrosine phosphatasc PTP-1D In addition, IGF I also stimulates a complex formation between IRS-1 and PTP-1D and the enzymatic activity of the tyrosine phosphatasc. The vasoactive octapeptidc Ang II, acting via its G-protein coupled ATi receptor, also elicits a similar early signaling event in RASM cells. We demonstrate that Ang II (10 'M) induces the tyrosine phosphorylation of IRS-1 (3-4 fold increase, l min) and PTP-1D (3-4t fold increase, 5 min), the complex formation of PTP-1D and IRS-1, and the tyrosine phosphatasc activity of FTP-ID (3-4 fold, 5 min). Furthermore, whan we clcctroporatc anu-IRS-1 antibodies into RASM cells, the Ang ll-induced activation of FTP-ID and its complex formation with IRS-1 arc blocked These findings suggest that IRS-1 and FTP-1D are closely involved in early intraceUular IGF I and Ang U signalling events, hi addition, these results may also represent an alternative signaling pathway mediated by seven transmcmbranc G-protcin linked receptors like the Ang II AT! receptor, leading to cell growth and proliferation.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

Fingerprint

Insulin Receptor Substrate Proteins
angiotensins
Phosphorylation
Angiotensins
smooth muscle
myocytes
Smooth Muscle Myocytes
Tyrosine
tyrosine
Muscle
Rats
phosphorylation
Chemical activation
Cells
angiotensin II
insulin-like growth factor I
Insulin-Like Growth Factor I
rats
Angiotensin II
receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Angiotensin ii stimulates the tyrosine phosphorylation and activation of irs-i and ftp-id in rat aortic smooth muscle cells. / Marrero, Mano B.; Schicflcr, Elisabcch; Schieffer, Bernhard.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

Marrero, Mano B. ; Schicflcr, Elisabcch ; Schieffer, Bernhard. / Angiotensin ii stimulates the tyrosine phosphorylation and activation of irs-i and ftp-id in rat aortic smooth muscle cells. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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