Angiotensin Il-Induced binding of the at1 receptor with phospholipast1 C-γ

Mario B Marrero, M. Showkat Ali, Bernhard Schigfferi, Joyce B. Harp, Kenneth H. Bernstein, D. Brian N. Line

Research output: Contribution to journalArticle

Abstract

Angiotensin II (Ang II), acting via its G protein-linked AT1, receptor, induces the tyrosine phosphorylation and activation of phospholipase (PLC)-yl- In this study we show that both the AT, receptor itself and a glutathione S-transferase (GST) fusion protein containing the cytoplasmic, carboxyl-termina) 54 amino acids of the AT, receptor physically binds to PLC-yl in an Ang Il-dependent manner. Amino acid dcletional analysis and peptide competition experiments show that this association is dependent on tyrosine residue J1Y and the "''YIPP motif in the carboxyl-terminal tail of the AT, receptor and the carboxyl-terminal SH2 domain of PLC-yl. These results support the concept that G protein-linked receptors can physically associate with intracellular proteins, creating membrane-delimited signal transduction complexes in a manner similar to that observed with classic growth factor receptors.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - Dec 1 1997

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Angiotensins
Programmable logic controllers
GTP-Binding Proteins
Amino Acid Receptors
src Homology Domains
Growth Factor Receptors
Phospholipases
Glutathione Transferase
Angiotensin II
Tyrosine
Amino Acids
Signal Transduction
Signal transduction
Membrane Proteins
Phosphorylation
Peptides
Fusion reactions
Chemical activation
Association reactions
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Marrero, M. B., Showkat Ali, M., Schigfferi, B., Harp, J. B., Bernstein, K. H., & Line, D. B. N. (1997). Angiotensin Il-Induced binding of the at1 receptor with phospholipast1 C-γ. FASEB Journal, 11(9).

Angiotensin Il-Induced binding of the at1 receptor with phospholipast1 C-γ. / Marrero, Mario B; Showkat Ali, M.; Schigfferi, Bernhard; Harp, Joyce B.; Bernstein, Kenneth H.; Line, D. Brian N.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997.

Research output: Contribution to journalArticle

Marrero, MB, Showkat Ali, M, Schigfferi, B, Harp, JB, Bernstein, KH & Line, DBN 1997, 'Angiotensin Il-Induced binding of the at1 receptor with phospholipast1 C-γ', FASEB Journal, vol. 11, no. 9.
Marrero MB, Showkat Ali M, Schigfferi B, Harp JB, Bernstein KH, Line DBN. Angiotensin Il-Induced binding of the at1 receptor with phospholipast1 C-γ. FASEB Journal. 1997 Dec 1;11(9).
Marrero, Mario B ; Showkat Ali, M. ; Schigfferi, Bernhard ; Harp, Joyce B. ; Bernstein, Kenneth H. ; Line, D. Brian N. / Angiotensin Il-Induced binding of the at1 receptor with phospholipast1 C-γ. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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