ANPs effect on MARCKS and StAR phosphorylation in agonist-stimulated glomerulosa cells

R. A. Calle, W. B. Bollag, S. White, S. Betancourt-Calle, P. Kent

Research output: Contribution to journalArticle

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Abstract

Atrial natriuretic peptide (ANP) is a cardiac hormone that inhibits aldosterone secretion induced by all physiologic agonists. The purpose of this study is to explore ANP-induced changes in the phosphorylation of myristoylated alanine-rich C-kinase substrate (MARCKS) and the steroidogenic acute regulatory protein (StAR), in AngII or K+-stimulated glomerulosa cells. The data show that ANP completely inhibits the phosphorylation of MARCKS and partially inhibits that of StAR in cells stimulated with K+. ANP also partially inhibits MARCKS phosphorylation but does not affect StAR phosphorylation in cells stimulated with AngII. These effects appear to be cGMP-independent and at least partially dependent on inhibition of protein kinase C (PKC). To our knowledge, this is the first report of ANP modulating either MARCKS or StAR phosphorylation in [32P]-labeled cells. The data also support the hypothesis that ANP inhibits aldosterone secretion acting as a step involved in cholesterol transport to the mitochondria.

Original languageEnglish (US)
Pages (from-to)71-79
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume177
Issue number1-2
DOIs
StatePublished - May 25 2001

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Keywords

  • Adrenal glomerulosa
  • Aldosterone
  • Angiotensin II
  • Myristoylated alanine-rich C-kinase substrate
  • Potassium
  • Steroidogenic acute regulatory protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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