Anterograde or retrograde transsynaptic circuit tracing in vertebrates with vesicular stomatitis virus vectors

Kevin T. Beier, Nathan A. Mundell, Yu Chin Albert Pan, Constance L. Cepko

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Viruses have been used as transsynaptic tracers, allowing one to map the inputs and outputs of neuronal populations, due to their ability to replicate in neurons and transmit in vivo only across synaptically connected cells. To date, their use has been largely restricted to mammals. In order to explore the use of such viruses in an expanded host range, we tested the transsynaptic tracing ability of recombinant vesicular stomatitis virus (rVSV) vectors in a variety of organisms. Successful infection and gene expression were achieved in a wide range of organisms, including vertebrate and invertebrate model organisms. Moreover, rVSV enabled transsynaptic tracing of neural circuitry in predictable directions dictated by the viral envelope glycoprotein (G), derived from either VSV or rabies virus (RABV). Anterograde and retrograde labeling, from initial infection and/or viral replication and transmission, was observed in Old and NewWorld monkeys, seahorses, jellyfish, zebrafish, chickens, and mice. These vectors are widely applicable for gene delivery, afferent tract tracing, and/or directional connectivity mapping. Here, we detail the use of these vectors and provide protocols for propagating virus, changing the surface glycoprotein, and infecting multiple organisms using several injection strategies.

Original languageEnglish (US)
Pages (from-to)1.26.1-1.26.27
JournalCurrent Protocols in Neuroscience
Volume2016
DOIs
StatePublished - Jan 1 2016

Fingerprint

Vesicular Stomatitis
Vertebrates
Viruses
Smegmamorpha
Rabies virus
Host Specificity
Membrane Glycoproteins
Zebrafish
Virus Diseases
Invertebrates
Haplorhini
Mammals
Chickens
Glycoproteins
Gene Expression
Neurons
Injections
Infection
Population
Genes

Keywords

  • Axon tracing
  • Gene delivery
  • Neural circuitry
  • Transsynaptic tracing
  • VSV

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Anterograde or retrograde transsynaptic circuit tracing in vertebrates with vesicular stomatitis virus vectors. / Beier, Kevin T.; Mundell, Nathan A.; Pan, Yu Chin Albert; Cepko, Constance L.

In: Current Protocols in Neuroscience, Vol. 2016, 01.01.2016, p. 1.26.1-1.26.27.

Research output: Contribution to journalArticle

Beier, Kevin T. ; Mundell, Nathan A. ; Pan, Yu Chin Albert ; Cepko, Constance L. / Anterograde or retrograde transsynaptic circuit tracing in vertebrates with vesicular stomatitis virus vectors. In: Current Protocols in Neuroscience. 2016 ; Vol. 2016. pp. 1.26.1-1.26.27.
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