Anti-angiogenic effect of auranofin on HUVECs in vitro and zebrafish in vivo

Ming Fang He, Xiao Ping Gao, Shu Chun Li, Zhi Heng He, Ning Chen, Yu Bin Wang, Jin-Xiong She

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Angiogenesis plays an essential role in many physiological and pathological processes. Auranofin (Ridaura®), an important gold(I) complex, is used to treat rheumatoid arthritis. However, the effect of auranofin on blood vessel formation is still unclear. In this study, we investigated the anti-angiogenic activity of auranofin on human umbilical vein endothelial cells (HUVEC) in vitro and zebrafish in vivo. Our results showed that auranofin could inhibit the proliferation, migration and tube formation of HUVECs and disrupted the formation of intersegmental vessels and the subintestinal vessels of zebrafish embryos. Auranofin inhibited the phosphorylation of vascular endothelial growth factor 2 (p-VEGFR2) on HUVECs and suppressed the vascular endothelial growth factor (VEGF) signaling pathway (vegfa, flt-1, kdr) but not thioredoxin reductase (TrxR) on zebrafish. Our study suggested that auranofin might serve as a potential anti-angiogenic compound candidate.

Original languageEnglish (US)
Pages (from-to)240-247
Number of pages8
JournalEuropean Journal of Pharmacology
Volume740
DOIs
StatePublished - Jan 1 2014

Fingerprint

Auranofin
Zebrafish
Vascular Endothelial Growth Factor A
Physiological Phenomena
Thioredoxin-Disulfide Reductase
Human Umbilical Vein Endothelial Cells
Pathologic Processes
In Vitro Techniques
Gold
Blood Vessels
Rheumatoid Arthritis
Embryonic Structures
Phosphorylation

Keywords

  • Angiogenesis inhibitor
  • Auranofin
  • HUVEC
  • Zebrafish

ASJC Scopus subject areas

  • Pharmacology

Cite this

Anti-angiogenic effect of auranofin on HUVECs in vitro and zebrafish in vivo. / He, Ming Fang; Gao, Xiao Ping; Li, Shu Chun; He, Zhi Heng; Chen, Ning; Wang, Yu Bin; She, Jin-Xiong.

In: European Journal of Pharmacology, Vol. 740, 01.01.2014, p. 240-247.

Research output: Contribution to journalArticle

He, Ming Fang ; Gao, Xiao Ping ; Li, Shu Chun ; He, Zhi Heng ; Chen, Ning ; Wang, Yu Bin ; She, Jin-Xiong. / Anti-angiogenic effect of auranofin on HUVECs in vitro and zebrafish in vivo. In: European Journal of Pharmacology. 2014 ; Vol. 740. pp. 240-247.
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AU - Wang, Yu Bin

AU - She, Jin-Xiong

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