Anti-apoptotic and Anti-inflammatory effect of Piperine on 6-OHDA induced Parkinson's Rat model

Pallavi Shrivastava, Kumar Vaibhav, Rizwana Tabassum, Andleeb Khan, Tauheed Ishrat, Mohd Moshahid Khan, Ajmal Ahmad, Farah Islam, Mohammed M. Safhi, Fakhrul Islam

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

In the present study, we examined the molecular mechanism by which Piperine (bioactive compound of Piper nigrum) inhibits neuronal cell apoptosis. We further investigated the anti-inflammatory effect of Piperine on 6-OHDA induced Parkinson's disease. Consistent with its antioxidant properties, Piperine (10. mg/kg bwt) reduced 6-OHDA-induced lipid peroxidation and stimulated glutathione levels in striatum of rats. Furthermore, Piperine treatment diminished cytochrome-c release from mitochondria and reduced caspase-3 and caspase-9 activation induced by 6-OHDA. Treatment with Piperine markedly inhibited poly(ADP-ribose) polymerase activation, pro-apoptotic Bax levels and elevation of Bcl-2 levels. Piperine reduces contralateral rotations induced by apomorphine. Further narrow beam test and rotarod also showed improvement in motor coordination and balance behavior in rats treated with Piperine. In addition Piperine depletes inflammatory markers, TNF-α and IL-1β in 6-OHDA-induced Parkinson's rats. We propose that, in addition to its antioxidant properties Piperine exerts a protective effect via anti-apoptotic and anti-inflammatory mechanism on 6-OHDA induced Parkinson's disease.

Original languageEnglish (US)
Pages (from-to)680-687
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume24
Issue number4
DOIs
Publication statusPublished - Apr 1 2013

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Keywords

  • 6-OHDA
  • Caspase-3&9
  • Cytochrome-c
  • IL-1β
  • Piperine
  • Poly(ADP-ribose) polymerase
  • TNF-α

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

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