Anti-biofilm efficacy of root canal irrigants against in-situ Enterococcus faecalis biofilms in root canals, isthmuses and dentinal tubules

Wei hu Ye, Bing Fan, Wiley Purcell, Mohamed M. Meghil, Christopher W Cutler, Brian Edward Bergeron, Jing zhi Ma, Franklin Chi Meng Tay, Li na Niu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: To investigate the anti-biofilm efficacy of root canal irrigants in canal spaces, isthmi and dentinal tubules of root canals ex vivo. Methods: Fifty-one single-rooted premolars, each containing an isthmus, were instrumented, autoclaved and inoculated with Enterococcus faecalis for 4 weeks. One specimen was sectioned for bacteria-specific staining to confirm the presence of biofilms using light microscopiy. The remaining specimens were randomly divided to five groups: (1) 0.9% NaCl, (2) SilverSol/H2O2, (3) HYBENX, (4) QMix 2 in1, (5) 6% NaOCl. Bacterial sampling was performed before (S1) and after (S2) canal irrigation. Diluted bacteria suspension was cultured for 48 h for counting the colony forming units (CFU). Percentages of dead bacteria and biofilm thickness were evaluated by confocal laser scanning microscopy (CLSM). Metabolic activity, lactic acid and polysaccharide synthesis of E. faecalis derived from S2 samples were analysed. Results: The percentages of dead bacteria were significantly affected by the factor “irrigant” (p < 0.001) and the factor “location” (p = 0.017). The percentages of dead bacteria in the isthmi and canals were both in the ordor: NaCl < SilverSol/H2O2 < HYBENX < QMix 2 in1 < NaOCl (p < 0.05). Only 6% NaOCl disrupted biofilms and significantly reduced their thickness. The CFU, metabolic activity, polysaccharide and lactic acid production of E. faecalis were all reduced by the disinfecting solutions. Conclusions: SilverSol/H2O2 and HYBENX were less adept than QMix 2 in1 at killing biofilm bacteria in root canals. None of these antibacterial irrigants were effective, compared with 6% NaOCl, in disrupting biofilms. Clinical significance: There is advantage in using HYBENX or QMix 2 in1 to kill intratubular bacteria biofilms because of their capability in removing the inorganic component of the smear layer. SilverSol/H2O2 requires extra time to eradicate intratubular biofilms upon removal of the organic and inorganic components of the smear layer by other root canal irrigants.

Original languageEnglish (US)
Pages (from-to)68-76
Number of pages9
JournalJournal of Dentistry
Volume79
DOIs
StatePublished - Dec 1 2018

Fingerprint

Root Canal Irrigants
Enterococcus faecalis
Dental Pulp Cavity
Biofilms
Bacteria
Smear Layer
Polysaccharides
Lactic Acid
Stem Cells
Bicuspid
Confocal Microscopy
Suspensions
Staining and Labeling

Keywords

  • Biofilm
  • Canal wall
  • Dentinal tubules
  • Isthmus
  • Lactic acid
  • Polysaccharide

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Anti-biofilm efficacy of root canal irrigants against in-situ Enterococcus faecalis biofilms in root canals, isthmuses and dentinal tubules. / Ye, Wei hu; Fan, Bing; Purcell, Wiley; Meghil, Mohamed M.; Cutler, Christopher W; Bergeron, Brian Edward; Ma, Jing zhi; Tay, Franklin Chi Meng; Niu, Li na.

In: Journal of Dentistry, Vol. 79, 01.12.2018, p. 68-76.

Research output: Contribution to journalArticle

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abstract = "Objective: To investigate the anti-biofilm efficacy of root canal irrigants in canal spaces, isthmi and dentinal tubules of root canals ex vivo. Methods: Fifty-one single-rooted premolars, each containing an isthmus, were instrumented, autoclaved and inoculated with Enterococcus faecalis for 4 weeks. One specimen was sectioned for bacteria-specific staining to confirm the presence of biofilms using light microscopiy. The remaining specimens were randomly divided to five groups: (1) 0.9{\%} NaCl, (2) SilverSol/H2O2, (3) HYBENX, (4) QMix 2 in1, (5) 6{\%} NaOCl. Bacterial sampling was performed before (S1) and after (S2) canal irrigation. Diluted bacteria suspension was cultured for 48 h for counting the colony forming units (CFU). Percentages of dead bacteria and biofilm thickness were evaluated by confocal laser scanning microscopy (CLSM). Metabolic activity, lactic acid and polysaccharide synthesis of E. faecalis derived from S2 samples were analysed. Results: The percentages of dead bacteria were significantly affected by the factor “irrigant” (p < 0.001) and the factor “location” (p = 0.017). The percentages of dead bacteria in the isthmi and canals were both in the ordor: NaCl < SilverSol/H2O2 < HYBENX < QMix 2 in1 < NaOCl (p < 0.05). Only 6{\%} NaOCl disrupted biofilms and significantly reduced their thickness. The CFU, metabolic activity, polysaccharide and lactic acid production of E. faecalis were all reduced by the disinfecting solutions. Conclusions: SilverSol/H2O2 and HYBENX were less adept than QMix 2 in1 at killing biofilm bacteria in root canals. None of these antibacterial irrigants were effective, compared with 6{\%} NaOCl, in disrupting biofilms. Clinical significance: There is advantage in using HYBENX or QMix 2 in1 to kill intratubular bacteria biofilms because of their capability in removing the inorganic component of the smear layer. SilverSol/H2O2 requires extra time to eradicate intratubular biofilms upon removal of the organic and inorganic components of the smear layer by other root canal irrigants.",
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author = "Ye, {Wei hu} and Bing Fan and Wiley Purcell and Meghil, {Mohamed M.} and Cutler, {Christopher W} and Bergeron, {Brian Edward} and Ma, {Jing zhi} and Tay, {Franklin Chi Meng} and Niu, {Li na}",
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AU - Fan, Bing

AU - Purcell, Wiley

AU - Meghil, Mohamed M.

AU - Cutler, Christopher W

AU - Bergeron, Brian Edward

AU - Ma, Jing zhi

AU - Tay, Franklin Chi Meng

AU - Niu, Li na

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