Anti-inflammatory effects of miR-21 in the macrophage response to peritonitis

Rebecca Elise Barnett, Daniel J. Conklin, Lindsey Ryan, Robert C. Keskey, Vikram Ramjee, Ernesto A. Sepulveda, Sanjay Srivastava, Aruni Bhatnagar, William G. Cheadle

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41 Scopus citations

Abstract

We investigated the role of microRNA-21 in the macrophage response to peritonitis; microRNA-21 expression increases in peritoneal macrophages after lipopolysaccharide stimulation but is delayed until 48 hours after cecal ligation and puncture. MicroRNA-21–null mice and bone marrow–derived cell lines were exposed to cecal ligation and puncture or lipopolysaccharide, and survival, microRNA-21 levels, target messenger RNAs and proteins, and cytokines were assayed. Macrophages were also transfected with microRNA-21 mimics and antagomirs, and similar endpoints were measured. Survival in microRNA-21–null mice was significantly decreased after lipopolysaccharide-induced peritonitis but unchanged after cecal ligation and puncture compared with similarly treated wild-type mice. MicroRNA-21 expression, tumor necrosis factor-α, interleukin 6, and programmed cell death protein 4 levels were increased after lipopolysaccharide addition in peritoneal cells. Pelino1 and sprouty (SPRY) messenger RNAs were similarly increased early, whereas programmed cell death protein 4 messenger RNA was decreased after lipopolysaccharide, and all microR-21 target messenger RNAs were subsequently decreased by 24 hours after lipopolysaccharide. Transfection with mimics and antagomirs led to appropriate responses in microRNA-21 and tumor necrosis factor-α. Knockdown of microRNA-21 in bone marrow–derived cells showed increased tumor necrosis factor-α and decreased interleukin 10 in response to lipopolysaccharide. Target proteins were unaffected by knockdown as was extracellular signal-regulated kinase; however, the nuclear factor κB p65 subunit was increased after lipopolysaccharide in the microRNA-21 knockout cells. In contrast, there was little change in these parameters after cecal ligation and puncture induction between null and wild-type mice. MicroRNA-21 is beneficial to survival in mice following lipopolysaccharide peritonitis. Overexpression of microRNA-21 decreased tumor necrosis factor-α secretion, whereas suppression of microRNA-21 expression increased tumor necrosis factor-α and interleukin 6, and decreased interleukin 10 levels after lipopolysaccharide. Protein targets of microRNA-21 were not different following suppression of microRNA-21. Nuclear factor κB was increased by suppression of microRNA-21. These findings demonstrate microRNA-21 is beneficial in modulating the macrophage response to lipopolysaccharide peritonitis and an improved understanding of the anti-inflammatory effects of microRNA-21 may result in novel, targeted therapy against peritonitis and sepsis.

Original languageEnglish (US)
Pages (from-to)361-371
Number of pages11
JournalJournal of Leukocyte Biology
Volume99
Issue number2
DOIs
StatePublished - Feb 2016

Keywords

  • Endotoxin
  • Inflammation
  • Septic shock
  • Toll-like receptor-4

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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    Barnett, R. E., Conklin, D. J., Ryan, L., Keskey, R. C., Ramjee, V., Sepulveda, E. A., Srivastava, S., Bhatnagar, A., & Cheadle, W. G. (2016). Anti-inflammatory effects of miR-21 in the macrophage response to peritonitis. Journal of Leukocyte Biology, 99(2), 361-371. https://doi.org/10.1189/jlb.4A1014-489R