Anti-Sulfoglucuronosyl Paragloboside Antibody

A Potential Serologic Marker of Amyotrophic Lateral Sclerosis

Dongpei Li, Seigo Usuki, Brandy Quarles, Michael H. Rivner, Toshio Ariga, Robert K. Yu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive degeneration of upper and lower motor neurons. Although the etiology of ALS is obscure, genetic studies of familiar ALS suggest a multifactorial etiology for this condition. Similarly, there probably are multiple causes for sporadic ALS. Autoimmune-mediated motor neuron dysfunction is one proposed etiology for sporadic ALS. In the present study, anti-glycolipid antibodies including GM1, GD1b, GD3, and sulfoglucuronosyl paragloboside (SGPG) were investigated in the sera of a large number of patient samples, including 113 ALS patients and 50 healthy controls, by means of enzyme-linked immunosorbent assay with affinity parametric complex criterion evaluation and thin-layer chromatography immunooverlay (immuno-TLC). Anti-SGPG antibodies were found in the sera of 13.3% ALS patients (15 out of 113). The highest titer reached 1:1600. The presence of anti-SGPG antibodies in the serum samples was also confirmed by immuno-TLC. Importantly, a multiple logistic regression analysis showed that the presence of anti-SGPG antibody was positively correlated with age (p <.01) and negatively correlated with ALS Functional Rating Scale score (p <.05). Moreover, the localization of SGPG-immunoreactivity on the motor neurons of rat spinal cord and a mouse motor neuronal cell line, NSC-34 was observed by an immunofluorescence method. These data suggest that SGPG could represent a specific pathogenic antigen in those ALS patients. The presence of anti-SGPG antibodies in the serum of ALS patients should represent a diagnostic biomarker of ALS, and it could reflect the severity of the disease.

Original languageEnglish (US)
JournalASN Neuro
Volume8
Issue number5
DOIs
StatePublished - Sep 1 2016

Fingerprint

Amyotrophic Lateral Sclerosis
Antibodies
Motor Neurons
Serum
sulfate-3-glucuronyl paragloboside
Glycolipids
Thin Layer Chromatography
Neurodegenerative Diseases
Fluorescent Antibody Technique
Anti-Idiotypic Antibodies
Spinal Cord
Biomarkers
Logistic Models
Enzyme-Linked Immunosorbent Assay
Regression Analysis
Antigens
Cell Line

Keywords

  • Functional Rating Scale
  • affinity parametric complex criterion
  • amyotrophic lateral sclerosis
  • anti-glycolipid antibody
  • ganglioside
  • sulfoglucuronosyl paragloboside

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

Cite this

Anti-Sulfoglucuronosyl Paragloboside Antibody : A Potential Serologic Marker of Amyotrophic Lateral Sclerosis. / Li, Dongpei; Usuki, Seigo; Quarles, Brandy; Rivner, Michael H.; Ariga, Toshio; Yu, Robert K.

In: ASN Neuro, Vol. 8, No. 5, 01.09.2016.

Research output: Contribution to journalArticle

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abstract = "Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive degeneration of upper and lower motor neurons. Although the etiology of ALS is obscure, genetic studies of familiar ALS suggest a multifactorial etiology for this condition. Similarly, there probably are multiple causes for sporadic ALS. Autoimmune-mediated motor neuron dysfunction is one proposed etiology for sporadic ALS. In the present study, anti-glycolipid antibodies including GM1, GD1b, GD3, and sulfoglucuronosyl paragloboside (SGPG) were investigated in the sera of a large number of patient samples, including 113 ALS patients and 50 healthy controls, by means of enzyme-linked immunosorbent assay with affinity parametric complex criterion evaluation and thin-layer chromatography immunooverlay (immuno-TLC). Anti-SGPG antibodies were found in the sera of 13.3{\%} ALS patients (15 out of 113). The highest titer reached 1:1600. The presence of anti-SGPG antibodies in the serum samples was also confirmed by immuno-TLC. Importantly, a multiple logistic regression analysis showed that the presence of anti-SGPG antibody was positively correlated with age (p <.01) and negatively correlated with ALS Functional Rating Scale score (p <.05). Moreover, the localization of SGPG-immunoreactivity on the motor neurons of rat spinal cord and a mouse motor neuronal cell line, NSC-34 was observed by an immunofluorescence method. These data suggest that SGPG could represent a specific pathogenic antigen in those ALS patients. The presence of anti-SGPG antibodies in the serum of ALS patients should represent a diagnostic biomarker of ALS, and it could reflect the severity of the disease.",
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