Antiadhesion effects of docosahexaenoic acid on normal human peritoneal and adhesion fibroblasts

Rahi Victory, Ghassan M. Saed, Michael Peter Diamond

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Objective: To determine whether docosahexaenoic acid (DHA) reduces adhesion marker mRNA levels in normal peritoneal and adhesion fibroblasts. Design: Prospective experimental study. Setting: University Medical Center. Patient(s): Three patients undergoing laparotomy with excision of adhesions and normal peritoneum. Intervention(s): DHA treatment (100 μM) of cell cultures for 24 hours. Main Outcome Measure(s): Real-time reverse transcriptase polymerase chain reaction (RT-PCR) quantification of relative changes (mRNA copies/μg mRNA) in mRNA levels of type I collagen, vascular endothelial growth factor (VEGF), and transforming growth factor-β1 (TGF-β1). Result(s): The DHA treatment significantly reduced type I collagen and VEGF, but not TGF-β1 mRNA levels in normal peritoneal fibroblasts compared to normal controls. The DHA treatment of adhesion fibroblasts reduced type I collagen mRNAs to those of normal peritoneal fibroblasts, decreasing mRNAs by 35% compared to untreated adhesion fibroblasts. The VEGF mRNA levels were 50% lower in DHA-treated adhesion fibroblasts versus untreated adhesion fibroblasts. Docasahexaenoic acid reduced TGF- β1 mRNA to normal levels in treated adhesion fibroblasts compared to untreated normal peritoneal fibroblasts. Conclusion(s): Docasahexaenoic acid substantially reduces levels of adhesion-related markers in normal peritoneal and adhesion fibroblasts. This study provides the molecular basis for an easily administered and potentially, highly efficacious, antiadhesion adjuvant.

Original languageEnglish (US)
Pages (from-to)1657-1662
Number of pages6
JournalFertility and Sterility
Volume88
Issue number6
DOIs
StatePublished - Dec 1 2007
Externally publishedYes

Keywords

  • DHA
  • Docosahexaenoic acid
  • adhesion barriers
  • adhesions
  • inflammation
  • omega 3
  • peritoneum
  • transforming growth factor-β1
  • type I collagen
  • vascular endothelial growth factor

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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