Antibodies to sulfated glucuronic acid containing glycosphingolipids in neuropathy associated with anti-MAG antibodies and in normal subjects

Scott McGinnis, Tatsuo Kohriyama, Robert K Yu, Michael A. Pesce, Norman Latov

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Serum of patients with neuropathy and IgM monoclonal antibodies (M-proteins) that bind to the myelin-associated glycoprotein (MAG) were tested for binding to the major cross-reactive sulfated glucuronic acid containing glycosphingolipid, sulfated glucuronic acid paragloboside (SGPG). IgM binding to the glycolipid was detectable at serum dilutions of 1 : 10 000 and reactivity was greatest at 4°C. Low titers of IgM binding to the glycolipid were also detected in sera from normal subjects and from patients with neurologic or rheumatologic diseases without serum M-proteins. Binding activity was prosent in 25% of the sera tested, and titers ranged between 1 : 25 and 1 : 400. One patient with peripheral neuropathy, however, had a measurable titer of 1 : 12 800 in the absence of monoclonal gammopathy. The study indicates that cold reacting anti-SGPG IgM antibodies are frequent constituents of the normal human antibody repertoire, and that monoclonal or polyclonal expansion of B cells that secrete these antibodies, is associated with peripheral neuropathy.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalJournal of Neuroimmunology
Volume17
Issue number2
DOIs
StatePublished - Jan 1 1988

Fingerprint

Myelin-Associated Glycoprotein
Glycosphingolipids
Glucuronic Acid
Immunoglobulin M
Antibodies
Glycolipids
Peripheral Nervous System Diseases
Serum
Monoclonal Antibodies
Paraproteinemias
Nervous System
Blood Proteins
B-Lymphocytes
Proteins
paragloboside

Keywords

  • Antibody
  • Autoantibody
  • Myelin-associated glycoprotein
  • Peripheral neuropathy
  • Sulfated glucuronic acid paragloboside

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Antibodies to sulfated glucuronic acid containing glycosphingolipids in neuropathy associated with anti-MAG antibodies and in normal subjects. / McGinnis, Scott; Kohriyama, Tatsuo; Yu, Robert K; Pesce, Michael A.; Latov, Norman.

In: Journal of Neuroimmunology, Vol. 17, No. 2, 01.01.1988, p. 119-126.

Research output: Contribution to journalArticle

@article{2a72730fa42347a5bd805aded654429c,
title = "Antibodies to sulfated glucuronic acid containing glycosphingolipids in neuropathy associated with anti-MAG antibodies and in normal subjects",
abstract = "Serum of patients with neuropathy and IgM monoclonal antibodies (M-proteins) that bind to the myelin-associated glycoprotein (MAG) were tested for binding to the major cross-reactive sulfated glucuronic acid containing glycosphingolipid, sulfated glucuronic acid paragloboside (SGPG). IgM binding to the glycolipid was detectable at serum dilutions of 1 : 10 000 and reactivity was greatest at 4°C. Low titers of IgM binding to the glycolipid were also detected in sera from normal subjects and from patients with neurologic or rheumatologic diseases without serum M-proteins. Binding activity was prosent in 25{\%} of the sera tested, and titers ranged between 1 : 25 and 1 : 400. One patient with peripheral neuropathy, however, had a measurable titer of 1 : 12 800 in the absence of monoclonal gammopathy. The study indicates that cold reacting anti-SGPG IgM antibodies are frequent constituents of the normal human antibody repertoire, and that monoclonal or polyclonal expansion of B cells that secrete these antibodies, is associated with peripheral neuropathy.",
keywords = "Antibody, Autoantibody, Myelin-associated glycoprotein, Peripheral neuropathy, Sulfated glucuronic acid paragloboside",
author = "Scott McGinnis and Tatsuo Kohriyama and Yu, {Robert K} and Pesce, {Michael A.} and Norman Latov",
year = "1988",
month = "1",
day = "1",
doi = "10.1016/0165-5728(88)90019-7",
language = "English (US)",
volume = "17",
pages = "119--126",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Antibodies to sulfated glucuronic acid containing glycosphingolipids in neuropathy associated with anti-MAG antibodies and in normal subjects

AU - McGinnis, Scott

AU - Kohriyama, Tatsuo

AU - Yu, Robert K

AU - Pesce, Michael A.

AU - Latov, Norman

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Serum of patients with neuropathy and IgM monoclonal antibodies (M-proteins) that bind to the myelin-associated glycoprotein (MAG) were tested for binding to the major cross-reactive sulfated glucuronic acid containing glycosphingolipid, sulfated glucuronic acid paragloboside (SGPG). IgM binding to the glycolipid was detectable at serum dilutions of 1 : 10 000 and reactivity was greatest at 4°C. Low titers of IgM binding to the glycolipid were also detected in sera from normal subjects and from patients with neurologic or rheumatologic diseases without serum M-proteins. Binding activity was prosent in 25% of the sera tested, and titers ranged between 1 : 25 and 1 : 400. One patient with peripheral neuropathy, however, had a measurable titer of 1 : 12 800 in the absence of monoclonal gammopathy. The study indicates that cold reacting anti-SGPG IgM antibodies are frequent constituents of the normal human antibody repertoire, and that monoclonal or polyclonal expansion of B cells that secrete these antibodies, is associated with peripheral neuropathy.

AB - Serum of patients with neuropathy and IgM monoclonal antibodies (M-proteins) that bind to the myelin-associated glycoprotein (MAG) were tested for binding to the major cross-reactive sulfated glucuronic acid containing glycosphingolipid, sulfated glucuronic acid paragloboside (SGPG). IgM binding to the glycolipid was detectable at serum dilutions of 1 : 10 000 and reactivity was greatest at 4°C. Low titers of IgM binding to the glycolipid were also detected in sera from normal subjects and from patients with neurologic or rheumatologic diseases without serum M-proteins. Binding activity was prosent in 25% of the sera tested, and titers ranged between 1 : 25 and 1 : 400. One patient with peripheral neuropathy, however, had a measurable titer of 1 : 12 800 in the absence of monoclonal gammopathy. The study indicates that cold reacting anti-SGPG IgM antibodies are frequent constituents of the normal human antibody repertoire, and that monoclonal or polyclonal expansion of B cells that secrete these antibodies, is associated with peripheral neuropathy.

KW - Antibody

KW - Autoantibody

KW - Myelin-associated glycoprotein

KW - Peripheral neuropathy

KW - Sulfated glucuronic acid paragloboside

UR - http://www.scopus.com/inward/record.url?scp=0023815816&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023815816&partnerID=8YFLogxK

U2 - 10.1016/0165-5728(88)90019-7

DO - 10.1016/0165-5728(88)90019-7

M3 - Article

C2 - 2447123

AN - SCOPUS:0023815816

VL - 17

SP - 119

EP - 126

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 2

ER -