Antibody responses in watermelon sensitivity

Robert N. Enberg, Judith McCullough, Dennis Randall Ownby

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Watermelon-specific IgE (WM-IgE) appears to mediate the oropharyngeal symptoms after watermelon ingestion. However, less than one fourth of watermelon-sensitive individuals (i.e., those with detectable WM-IgE) develop such symptoms after watermelon ingestion. In an attempt to separate symptomatic from asymptomatic persons, we measured watermelon-specific-IgE and IgG4 concentrations in the sera of 29 watermelon-sensitive patients, 6 of whom were symptomatic. Although the mean WM-IgE level of the symptomatic group exceeded that of the asymptomatic group (p = 0.04), individual WM-IgE values failed to distinguish symptomatic from asymptomatic persons. No differences in WM-IgG4 levels between the two groups could be found. Watermelon extract proteins were separated by isoelectric focusing and blotted to nitrocellulose membranes. The membranes were reacted with the individual sera of 14 watermelon-sensitive patients and then stained for IgE, IgG1, and IgG4 binding. Great variations in IgE, IgG1, and IgG4 binding patterns were found, but no subtype restrictions were discovered. Although the pattern of IgE-stained bands in the absence of corresponding IgG1-stained bands was seen more frequently in symptomatic individuals, immunoblot patterns failed to separate symptomatic from asymptomatic persons. We conclude that the pattern of IgE, IgG1, and IgG4 responses to separated watermelon allergens was not predictive of clinical symptoms in a group of watermelon-sensitive patients.

Original languageEnglish (US)
Pages (from-to)795-800
Number of pages6
JournalThe Journal of Allergy and Clinical Immunology
Volume82
Issue number5 PART 1
DOIs
StatePublished - Jan 1 1988

Fingerprint

Citrullus
Antibody Formation
Immunoglobulin E
Immunoglobulin G
Eating
Collodion
Membranes
Isoelectric Focusing
Serum
Allergens

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Antibody responses in watermelon sensitivity. / Enberg, Robert N.; McCullough, Judith; Ownby, Dennis Randall.

In: The Journal of Allergy and Clinical Immunology, Vol. 82, No. 5 PART 1, 01.01.1988, p. 795-800.

Research output: Contribution to journalArticle

Enberg, Robert N. ; McCullough, Judith ; Ownby, Dennis Randall. / Antibody responses in watermelon sensitivity. In: The Journal of Allergy and Clinical Immunology. 1988 ; Vol. 82, No. 5 PART 1. pp. 795-800.
@article{496b4cd74aef4603884e7b3bfe20d041,
title = "Antibody responses in watermelon sensitivity",
abstract = "Watermelon-specific IgE (WM-IgE) appears to mediate the oropharyngeal symptoms after watermelon ingestion. However, less than one fourth of watermelon-sensitive individuals (i.e., those with detectable WM-IgE) develop such symptoms after watermelon ingestion. In an attempt to separate symptomatic from asymptomatic persons, we measured watermelon-specific-IgE and IgG4 concentrations in the sera of 29 watermelon-sensitive patients, 6 of whom were symptomatic. Although the mean WM-IgE level of the symptomatic group exceeded that of the asymptomatic group (p = 0.04), individual WM-IgE values failed to distinguish symptomatic from asymptomatic persons. No differences in WM-IgG4 levels between the two groups could be found. Watermelon extract proteins were separated by isoelectric focusing and blotted to nitrocellulose membranes. The membranes were reacted with the individual sera of 14 watermelon-sensitive patients and then stained for IgE, IgG1, and IgG4 binding. Great variations in IgE, IgG1, and IgG4 binding patterns were found, but no subtype restrictions were discovered. Although the pattern of IgE-stained bands in the absence of corresponding IgG1-stained bands was seen more frequently in symptomatic individuals, immunoblot patterns failed to separate symptomatic from asymptomatic persons. We conclude that the pattern of IgE, IgG1, and IgG4 responses to separated watermelon allergens was not predictive of clinical symptoms in a group of watermelon-sensitive patients.",
author = "Enberg, {Robert N.} and Judith McCullough and Ownby, {Dennis Randall}",
year = "1988",
month = "1",
day = "1",
doi = "10.1016/0091-6749(88)90081-4",
language = "English (US)",
volume = "82",
pages = "795--800",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "5 PART 1",

}

TY - JOUR

T1 - Antibody responses in watermelon sensitivity

AU - Enberg, Robert N.

AU - McCullough, Judith

AU - Ownby, Dennis Randall

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Watermelon-specific IgE (WM-IgE) appears to mediate the oropharyngeal symptoms after watermelon ingestion. However, less than one fourth of watermelon-sensitive individuals (i.e., those with detectable WM-IgE) develop such symptoms after watermelon ingestion. In an attempt to separate symptomatic from asymptomatic persons, we measured watermelon-specific-IgE and IgG4 concentrations in the sera of 29 watermelon-sensitive patients, 6 of whom were symptomatic. Although the mean WM-IgE level of the symptomatic group exceeded that of the asymptomatic group (p = 0.04), individual WM-IgE values failed to distinguish symptomatic from asymptomatic persons. No differences in WM-IgG4 levels between the two groups could be found. Watermelon extract proteins were separated by isoelectric focusing and blotted to nitrocellulose membranes. The membranes were reacted with the individual sera of 14 watermelon-sensitive patients and then stained for IgE, IgG1, and IgG4 binding. Great variations in IgE, IgG1, and IgG4 binding patterns were found, but no subtype restrictions were discovered. Although the pattern of IgE-stained bands in the absence of corresponding IgG1-stained bands was seen more frequently in symptomatic individuals, immunoblot patterns failed to separate symptomatic from asymptomatic persons. We conclude that the pattern of IgE, IgG1, and IgG4 responses to separated watermelon allergens was not predictive of clinical symptoms in a group of watermelon-sensitive patients.

AB - Watermelon-specific IgE (WM-IgE) appears to mediate the oropharyngeal symptoms after watermelon ingestion. However, less than one fourth of watermelon-sensitive individuals (i.e., those with detectable WM-IgE) develop such symptoms after watermelon ingestion. In an attempt to separate symptomatic from asymptomatic persons, we measured watermelon-specific-IgE and IgG4 concentrations in the sera of 29 watermelon-sensitive patients, 6 of whom were symptomatic. Although the mean WM-IgE level of the symptomatic group exceeded that of the asymptomatic group (p = 0.04), individual WM-IgE values failed to distinguish symptomatic from asymptomatic persons. No differences in WM-IgG4 levels between the two groups could be found. Watermelon extract proteins were separated by isoelectric focusing and blotted to nitrocellulose membranes. The membranes were reacted with the individual sera of 14 watermelon-sensitive patients and then stained for IgE, IgG1, and IgG4 binding. Great variations in IgE, IgG1, and IgG4 binding patterns were found, but no subtype restrictions were discovered. Although the pattern of IgE-stained bands in the absence of corresponding IgG1-stained bands was seen more frequently in symptomatic individuals, immunoblot patterns failed to separate symptomatic from asymptomatic persons. We conclude that the pattern of IgE, IgG1, and IgG4 responses to separated watermelon allergens was not predictive of clinical symptoms in a group of watermelon-sensitive patients.

UR - http://www.scopus.com/inward/record.url?scp=0024269773&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024269773&partnerID=8YFLogxK

U2 - 10.1016/0091-6749(88)90081-4

DO - 10.1016/0091-6749(88)90081-4

M3 - Article

C2 - 3192863

AN - SCOPUS:0024269773

VL - 82

SP - 795

EP - 800

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 5 PART 1

ER -