Antimicrobial efficacy of an apical negative pressure root canal irrigation system against intracanal microorganisms

Chang Zeng, Mohamed M. Meghil, Marcus Miller, Yaping Gou, Christopher W. Cutler, Brian E. Bergeron, Lina Niu, Jingzhi Ma, Franklin R. Tay

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: The effect of irrigation time on the antimicrobial efficacy of an apical negative pressure irrigation system was examined in vitro, followed by validation of the antimicrobial effect in vivo using the identified optimal irrigation time. Methods: For the in vitro experiment, 44 extracted premolars were decoronated, instrumented, autoclaved and inoculated with Enterococcus faecalis (ATCC 29212) for 21 days. Four teeth were used as positive control, without irrigation. Each of the remaining 40 teeth was irrigated with 2.5% NaOCl, delivered via the EndoVac MacroCannula for 10 s, and subsequently via the EndoVac MicroCannula for 15, 30, 45, 60 or 90 s per canal, respectively (N = 8). After irrigation, microbial samples were collected, transferred to BHI broth and incubated for counting of bacterial colony forming units (CFUs). Based on the in vitro results, 8.25% NaOCl was delivered via the EndoVac MicroCannula for 60 s, during root canal treatment of 20 human subjects presented with apical periodontitis. Microbial samples retrieved in vivo prior to canal instrumentation (S0), after chemomechanical debridement (S1) and after irrigation with EndoVac (S2) were cultured in an anaerobic chamber for 7 days for CFU evaluation. Results: Compared with the control, irrigation significantly reduced bacterial populations (p <.05). Irrigation delivery via the EndoVac demonstrated improved antibacterial efficacy with increased irrigation time (p <.05). Samples retrieved from canals after NaOCl delivery in vivo with the EndoVac for 60 s were all culture-negative. Conclusions: Microbial elimination may be achieved with 8.25% NaOCl delivered via the EndoVac apical negative pressure irrigation device for 60 s. Clinical significance: With the use of the EndoVac apical negative pressure irrigant delivery system, optimal elimination of the intracanal bacterial load can only be achieved when sodium hypochlorite is delivered via the MicroCannula for at least 60 s per canal.

Original languageEnglish (US)
Pages (from-to)71-75
Number of pages5
JournalJournal of Dentistry
Volume72
DOIs
StatePublished - May 2018

Fingerprint

Dental Pulp Cavity
Pressure
Tooth
Stem Cells
Periapical Periodontitis
Sodium Hypochlorite
Bacterial Load
Enterococcus faecalis
Bicuspid
Debridement
Equipment and Supplies
Population
In Vitro Techniques

Keywords

  • Antimicrobial
  • Apical negative pressure
  • Disinfection
  • Irrigation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Antimicrobial efficacy of an apical negative pressure root canal irrigation system against intracanal microorganisms. / Zeng, Chang; Meghil, Mohamed M.; Miller, Marcus; Gou, Yaping; Cutler, Christopher W.; Bergeron, Brian E.; Niu, Lina; Ma, Jingzhi; Tay, Franklin R.

In: Journal of Dentistry, Vol. 72, 05.2018, p. 71-75.

Research output: Contribution to journalArticle

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title = "Antimicrobial efficacy of an apical negative pressure root canal irrigation system against intracanal microorganisms",
abstract = "Objectives: The effect of irrigation time on the antimicrobial efficacy of an apical negative pressure irrigation system was examined in vitro, followed by validation of the antimicrobial effect in vivo using the identified optimal irrigation time. Methods: For the in vitro experiment, 44 extracted premolars were decoronated, instrumented, autoclaved and inoculated with Enterococcus faecalis (ATCC 29212) for 21 days. Four teeth were used as positive control, without irrigation. Each of the remaining 40 teeth was irrigated with 2.5{\%} NaOCl, delivered via the EndoVac MacroCannula for 10 s, and subsequently via the EndoVac MicroCannula for 15, 30, 45, 60 or 90 s per canal, respectively (N = 8). After irrigation, microbial samples were collected, transferred to BHI broth and incubated for counting of bacterial colony forming units (CFUs). Based on the in vitro results, 8.25{\%} NaOCl was delivered via the EndoVac MicroCannula for 60 s, during root canal treatment of 20 human subjects presented with apical periodontitis. Microbial samples retrieved in vivo prior to canal instrumentation (S0), after chemomechanical debridement (S1) and after irrigation with EndoVac (S2) were cultured in an anaerobic chamber for 7 days for CFU evaluation. Results: Compared with the control, irrigation significantly reduced bacterial populations (p <.05). Irrigation delivery via the EndoVac demonstrated improved antibacterial efficacy with increased irrigation time (p <.05). Samples retrieved from canals after NaOCl delivery in vivo with the EndoVac for 60 s were all culture-negative. Conclusions: Microbial elimination may be achieved with 8.25{\%} NaOCl delivered via the EndoVac apical negative pressure irrigation device for 60 s. Clinical significance: With the use of the EndoVac apical negative pressure irrigant delivery system, optimal elimination of the intracanal bacterial load can only be achieved when sodium hypochlorite is delivered via the MicroCannula for at least 60 s per canal.",
keywords = "Antimicrobial, Apical negative pressure, Disinfection, Irrigation",
author = "Chang Zeng and Meghil, {Mohamed M.} and Marcus Miller and Yaping Gou and Cutler, {Christopher W.} and Bergeron, {Brian E.} and Lina Niu and Jingzhi Ma and Tay, {Franklin R.}",
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T1 - Antimicrobial efficacy of an apical negative pressure root canal irrigation system against intracanal microorganisms

AU - Zeng, Chang

AU - Meghil, Mohamed M.

AU - Miller, Marcus

AU - Gou, Yaping

AU - Cutler, Christopher W.

AU - Bergeron, Brian E.

AU - Niu, Lina

AU - Ma, Jingzhi

AU - Tay, Franklin R.

PY - 2018/5

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N2 - Objectives: The effect of irrigation time on the antimicrobial efficacy of an apical negative pressure irrigation system was examined in vitro, followed by validation of the antimicrobial effect in vivo using the identified optimal irrigation time. Methods: For the in vitro experiment, 44 extracted premolars were decoronated, instrumented, autoclaved and inoculated with Enterococcus faecalis (ATCC 29212) for 21 days. Four teeth were used as positive control, without irrigation. Each of the remaining 40 teeth was irrigated with 2.5% NaOCl, delivered via the EndoVac MacroCannula for 10 s, and subsequently via the EndoVac MicroCannula for 15, 30, 45, 60 or 90 s per canal, respectively (N = 8). After irrigation, microbial samples were collected, transferred to BHI broth and incubated for counting of bacterial colony forming units (CFUs). Based on the in vitro results, 8.25% NaOCl was delivered via the EndoVac MicroCannula for 60 s, during root canal treatment of 20 human subjects presented with apical periodontitis. Microbial samples retrieved in vivo prior to canal instrumentation (S0), after chemomechanical debridement (S1) and after irrigation with EndoVac (S2) were cultured in an anaerobic chamber for 7 days for CFU evaluation. Results: Compared with the control, irrigation significantly reduced bacterial populations (p <.05). Irrigation delivery via the EndoVac demonstrated improved antibacterial efficacy with increased irrigation time (p <.05). Samples retrieved from canals after NaOCl delivery in vivo with the EndoVac for 60 s were all culture-negative. Conclusions: Microbial elimination may be achieved with 8.25% NaOCl delivered via the EndoVac apical negative pressure irrigation device for 60 s. Clinical significance: With the use of the EndoVac apical negative pressure irrigant delivery system, optimal elimination of the intracanal bacterial load can only be achieved when sodium hypochlorite is delivered via the MicroCannula for at least 60 s per canal.

AB - Objectives: The effect of irrigation time on the antimicrobial efficacy of an apical negative pressure irrigation system was examined in vitro, followed by validation of the antimicrobial effect in vivo using the identified optimal irrigation time. Methods: For the in vitro experiment, 44 extracted premolars were decoronated, instrumented, autoclaved and inoculated with Enterococcus faecalis (ATCC 29212) for 21 days. Four teeth were used as positive control, without irrigation. Each of the remaining 40 teeth was irrigated with 2.5% NaOCl, delivered via the EndoVac MacroCannula for 10 s, and subsequently via the EndoVac MicroCannula for 15, 30, 45, 60 or 90 s per canal, respectively (N = 8). After irrigation, microbial samples were collected, transferred to BHI broth and incubated for counting of bacterial colony forming units (CFUs). Based on the in vitro results, 8.25% NaOCl was delivered via the EndoVac MicroCannula for 60 s, during root canal treatment of 20 human subjects presented with apical periodontitis. Microbial samples retrieved in vivo prior to canal instrumentation (S0), after chemomechanical debridement (S1) and after irrigation with EndoVac (S2) were cultured in an anaerobic chamber for 7 days for CFU evaluation. Results: Compared with the control, irrigation significantly reduced bacterial populations (p <.05). Irrigation delivery via the EndoVac demonstrated improved antibacterial efficacy with increased irrigation time (p <.05). Samples retrieved from canals after NaOCl delivery in vivo with the EndoVac for 60 s were all culture-negative. Conclusions: Microbial elimination may be achieved with 8.25% NaOCl delivered via the EndoVac apical negative pressure irrigation device for 60 s. Clinical significance: With the use of the EndoVac apical negative pressure irrigant delivery system, optimal elimination of the intracanal bacterial load can only be achieved when sodium hypochlorite is delivered via the MicroCannula for at least 60 s per canal.

KW - Antimicrobial

KW - Apical negative pressure

KW - Disinfection

KW - Irrigation

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