Antioxidant effects of lipophilic tea polyphenols on diethylnitrosamine/phenobarbital-induced hepatocarcinogenesis in rats

Fan Zhou, Ting Shen, Ting Duan, Yu Ying Xu, Soo Chin Khor, Jin Li, Juan Ge, Yi Fan Zheng, Stephen Hsu, Jamie Ann De Stefano, Jun Yang, Li Hong Xu, Xin Qiang Zhu

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Abstract

Background: The purpose of the present study was to compare the antioxidant potential of lipophilic tea polyphenols (LTP) against the one of naturally-occurring watersoluble green tea polyphenols (GTP) in a two-stage model of diethylnitrosamine (DEN)/phenobarbital (PB)-induced hepatocarcinogenesis in Sprague-Dawley rats. Materials and Methods: GTP/LTP was given 5-times weekly by oral gavage with tea polyphenols equivalent to 0-, 40- and 400-mg/kg of body weight/day. GTP/LTP treatment was started 2 weeks prior to the initiation of DEN and continued for 30 weeks. Results: Histopathological and electron microscopic examination of liver tissue confirmed the protective effect of LTP on DEN/PBinduced liver damage and pre-carcinogenesis. LTP treatment significantly increased total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activity in liver tissues. Immunohistochemical detection of cellular nuclear factor erythroid-2-related factor-2 (Nrf2) and peroxiredoxin-6 (P6) indicated a down-regulation in Nrf2 and up-regulation of P6 expression in the liver of LTP-supplemented rats. Conclusion: The present study provides evidence for the first time, that LTP exerts significant antioxidant effects on DEN/PB-induced liver damage and hepatocarcinogenesis through elevating T-AOC levels, enhancing GSH-Px activity and inducing P6 expression in rat liver tissues.

Original languageEnglish (US)
Pages (from-to)495-504
Number of pages10
JournalIn Vivo
Volume28
Issue number4
StatePublished - Jan 1 2014

Fingerprint

Diethylnitrosamine
Polyphenols
Tea
Phenobarbital
Rats
Antioxidants
Liver
Peroxiredoxin VI
Tissue
Glutathione Peroxidase
Sprague Dawley Rats
Microscopic examination
Carcinogenesis
Up-Regulation
Down-Regulation
Body Weight

Keywords

  • Antioxidant capacity
  • Hepatocarcinogenesis
  • Nrf2
  • Peroxiredoxin 6
  • Tea polyphenols

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Zhou, F., Shen, T., Duan, T., Xu, Y. Y., Khor, S. C., Li, J., ... Zhu, X. Q. (2014). Antioxidant effects of lipophilic tea polyphenols on diethylnitrosamine/phenobarbital-induced hepatocarcinogenesis in rats. In Vivo, 28(4), 495-504.

Antioxidant effects of lipophilic tea polyphenols on diethylnitrosamine/phenobarbital-induced hepatocarcinogenesis in rats. / Zhou, Fan; Shen, Ting; Duan, Ting; Xu, Yu Ying; Khor, Soo Chin; Li, Jin; Ge, Juan; Zheng, Yi Fan; Hsu, Stephen; De Stefano, Jamie Ann; Yang, Jun; Xu, Li Hong; Zhu, Xin Qiang.

In: In Vivo, Vol. 28, No. 4, 01.01.2014, p. 495-504.

Research output: Contribution to journalArticle

Zhou, F, Shen, T, Duan, T, Xu, YY, Khor, SC, Li, J, Ge, J, Zheng, YF, Hsu, S, De Stefano, JA, Yang, J, Xu, LH & Zhu, XQ 2014, 'Antioxidant effects of lipophilic tea polyphenols on diethylnitrosamine/phenobarbital-induced hepatocarcinogenesis in rats', In Vivo, vol. 28, no. 4, pp. 495-504.
Zhou, Fan ; Shen, Ting ; Duan, Ting ; Xu, Yu Ying ; Khor, Soo Chin ; Li, Jin ; Ge, Juan ; Zheng, Yi Fan ; Hsu, Stephen ; De Stefano, Jamie Ann ; Yang, Jun ; Xu, Li Hong ; Zhu, Xin Qiang. / Antioxidant effects of lipophilic tea polyphenols on diethylnitrosamine/phenobarbital-induced hepatocarcinogenesis in rats. In: In Vivo. 2014 ; Vol. 28, No. 4. pp. 495-504.
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AU - Khor, Soo Chin

AU - Li, Jin

AU - Ge, Juan

AU - Zheng, Yi Fan

AU - Hsu, Stephen

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