Antioxidant N-acetylcysteine and AMPA/KA receptor antagonist DNQX inhibited mixed lineage kinase-3 activation following cerebral ischemia in rat hippocampus

Hui Tian, Quanguang Zhang, Hongchun Li, Guangyi Zhang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We measured the MLK3 expression, activity and backphosphorylation following cerebral ischemia. Our data showed that MLK3 protein levels were unalterable during ischemia and reperfusion. However, during ischemia MLK3 activity gradually increased and reached its peak at 30 min of ischemia. While its backphosphorylation reduced from 5 min of ischemia to 30 min of ischemia. In addition, we also detected MLK3 alteration at various time points of reperfusion after 15 min of ischemia, which showed that MLK3 activity increased twice, whereas MLK3 backphosphorylation was similarly consistent with its activity during reperfusion. To further analyze the reason of MLK3 activation, antioxidant N-acetylcysteine (NAC) and α-amino-3-hydroxy-5-methyl-4-isoxazole proprionate (AMPA)/kainate (KA) receptor antagonist 6,7-dinitroquinoxaline-2,3(1H, 4H)-dione (DNQX) were given to the rats 20 min prior to ischemia. The results illustrated that NAC preferably inhibited the MLK3 activation during the ischemia and the early reperfusion, whereas DNQX effectively attenuated the MLK3 activation of the late reperfusion. We think that MLK3 activation is certainly associated with reactive oxygen species (ROS) and AMPA/KA receptor in response to ischemic insult.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalNeuroscience Research
Volume47
Issue number1
DOIs
StatePublished - Sep 1 2003

Fingerprint

Kainic Acid Receptors
AMPA Receptors
Acetylcysteine
Brain Ischemia
Hippocampus
Ischemia
Antioxidants
Reperfusion
Isoxazoles
mitogen-activated protein kinase kinase kinase 11
FG 9041
Reactive Oxygen Species

Keywords

  • AMPA/KA receptor
  • Activation
  • Cerebral ischemia
  • DNQX
  • Mixed lineage kinase-3 (MLK3)
  • NAC
  • ROS
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Antioxidant N-acetylcysteine and AMPA/KA receptor antagonist DNQX inhibited mixed lineage kinase-3 activation following cerebral ischemia in rat hippocampus. / Tian, Hui; Zhang, Quanguang; Li, Hongchun; Zhang, Guangyi.

In: Neuroscience Research, Vol. 47, No. 1, 01.09.2003, p. 47-53.

Research output: Contribution to journalArticle

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