Antioxidant NAC and AMPA/KA receptor antagonist DNQX inhibited JNK3 activation following global ischemia in rat hippocampus

Hui Tian, Guangyi Zhang, Hongchun Li, Quanguang Zhang

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

c-Jun N-terminal kinase-3 (JNK3), the only neural-specific isoform, may play an important role in excitotoxicity and neuronal injury. To analyze the variation of JNK3 activation, levels of phospho-JNK3 were measured at various time points of ischemia and selected time points of reperfusion, respectively. Our study illustrated that JNK3 was rapidly activated and translocated from cytosol to nucleus during ischemia. During reperfusion, two peaks of JNK3 activation occurred at 30 min and 3 days, respectively. To further define the mechanism of JNK3 activation, antioxidant N-acetylcysteine (NAC), α-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate (KA) receptor antagonist 6,7-dinitro-quinoxaline-2,3(1H,4H)-dione (DNQX), N-methyl-D-aspartate (NMDA) receptor antagonist ketamine and L-type voltage-gated Ca2+ channel (L-VGCC) antagonist nifedipine were given to the rats 20 min prior to ischemia. The results showed that NAC obviously inhibited JNK3 activation during the early reperfusion, whereas DNQX preferably attenuated JNK3 activation during the latter reperfusion. Ketamine and nifedipine had no significant effects on JNK3 activation during reperfusion. Consequently, reactive oxygen species (ROS) and AMPA/KA receptor were closely associated with JNK3 activation following global ischemia.

Original languageEnglish (US)
Pages (from-to)191-197
Number of pages7
JournalNeuroscience Research
Volume46
Issue number2
DOIs
StatePublished - Jun 1 2003

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Mitogen-Activated Protein Kinase 10
Kainic Acid Receptors
AMPA Receptors
Acetylcysteine
Hippocampus
Ischemia
Antioxidants
Reperfusion
Ketamine
Nifedipine
FG 9041
Quinoxalines
N-Methyl-D-Aspartate Receptors
Hydroxyl Radical
Cytosol

Keywords

  • Activation
  • DNQX
  • JNK3
  • NAC
  • Nuclear translocation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Antioxidant NAC and AMPA/KA receptor antagonist DNQX inhibited JNK3 activation following global ischemia in rat hippocampus. / Tian, Hui; Zhang, Guangyi; Li, Hongchun; Zhang, Quanguang.

In: Neuroscience Research, Vol. 46, No. 2, 01.06.2003, p. 191-197.

Research output: Contribution to journalArticle

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