Antiproliferative and proapoptotic effects of epigallocatechin gallate on human leiomyoma cells

Dong Zhang, Mohamed Al-Hendy, Gloria Richard-Davis, Valerie Montgomery-Rice, Veera Rajaratnam, Ayman Al-Hendy

Research output: Contribution to journalArticle

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Abstract

Objective: To investigate the effects of epigallocatechin gallate (EGCG), an extract of green tea on cultured human leiomyoma cells (HuLM). Design: Laboratory study. Setting: University hospitals. Patient(s): Not applicable. Intervention(s): Not applicable. Main Outcome Measure(s): The HuLM cells were treated with various EGCG concentrations. Cell proliferation was assayed using Hoechst 33258 dye, and apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Total RNA was isolated, and gene expression profiling was performed on 84 key genes related to 18 different signal transduction pathways. The protein levels of PCNA, CDK4, BCL2, and BAX were examined by Western blot analysis. Result(s): The HuLM cells treated with EGCG showed a dose-dependent and time-dependent inhibition of cell proliferation. The TUNEL staining indicated a significant increase in apoptosis in HuLM cells treated with 100 μM of EGCG compared with untreated control. Gene expression profiling indicated that EGCG treatment up-regulated representative genes from the transforming growth factor β (TGF-β) and stress pathways, while inhibiting the survival pathway and NFκB-dependent inflammatory pathway. Western blot analysis confirmed that EGCG at ≥50 μM significantly decreased the expression of PCNA, CDK4, and BCL2 as well as increased the expression of the proapoptotic BAX in a dose-dependent manner. Conclusion(s): Epigallocatechin gallate inhibits the proliferation of HuLM cells and induces apoptosis. These results suggest that EGCG may be a potential anti-uterine fibroid agent acting through multiple signal transduction pathways.

Original languageEnglish (US)
Pages (from-to)1887-1893
Number of pages7
JournalFertility and Sterility
Volume94
Issue number5
DOIs
StatePublished - Oct 1 2010

Fingerprint

Leiomyoma
Proliferating Cell Nuclear Antigen
Gene Expression Profiling
Apoptosis
Signal Transduction
Western Blotting
Cell Proliferation
Bisbenzimidazole
epigallocatechin gallate
DNA Nucleotidylexotransferase
Transforming Growth Factors
Tea
Transferases
Genes
Coloring Agents
Outcome Assessment (Health Care)
RNA
Staining and Labeling
Survival

Keywords

  • Apoptosis
  • epigallocatechin gallate
  • leiomyoma
  • proliferation

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Zhang, D., Al-Hendy, M., Richard-Davis, G., Montgomery-Rice, V., Rajaratnam, V., & Al-Hendy, A. (2010). Antiproliferative and proapoptotic effects of epigallocatechin gallate on human leiomyoma cells. Fertility and Sterility, 94(5), 1887-1893. https://doi.org/10.1016/j.fertnstert.2009.08.065

Antiproliferative and proapoptotic effects of epigallocatechin gallate on human leiomyoma cells. / Zhang, Dong; Al-Hendy, Mohamed; Richard-Davis, Gloria; Montgomery-Rice, Valerie; Rajaratnam, Veera; Al-Hendy, Ayman.

In: Fertility and Sterility, Vol. 94, No. 5, 01.10.2010, p. 1887-1893.

Research output: Contribution to journalArticle

Zhang, D, Al-Hendy, M, Richard-Davis, G, Montgomery-Rice, V, Rajaratnam, V & Al-Hendy, A 2010, 'Antiproliferative and proapoptotic effects of epigallocatechin gallate on human leiomyoma cells', Fertility and Sterility, vol. 94, no. 5, pp. 1887-1893. https://doi.org/10.1016/j.fertnstert.2009.08.065
Zhang D, Al-Hendy M, Richard-Davis G, Montgomery-Rice V, Rajaratnam V, Al-Hendy A. Antiproliferative and proapoptotic effects of epigallocatechin gallate on human leiomyoma cells. Fertility and Sterility. 2010 Oct 1;94(5):1887-1893. https://doi.org/10.1016/j.fertnstert.2009.08.065
Zhang, Dong ; Al-Hendy, Mohamed ; Richard-Davis, Gloria ; Montgomery-Rice, Valerie ; Rajaratnam, Veera ; Al-Hendy, Ayman. / Antiproliferative and proapoptotic effects of epigallocatechin gallate on human leiomyoma cells. In: Fertility and Sterility. 2010 ; Vol. 94, No. 5. pp. 1887-1893.
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