TY - JOUR
T1 - Antiproliferative and proapoptotic effects of epigallocatechin gallate on human leiomyoma cells
AU - Zhang, Dong
AU - Al-Hendy, Mohamed
AU - Richard-Davis, Gloria
AU - Montgomery-Rice, Valerie
AU - Rajaratnam, Veera
AU - Al-Hendy, Ayman
N1 - Funding Information:
The apoptotic nuclei were stained with 1 μg/mL of propidium iodide solution (Sigma), and the slides were examined under an Eclipse TE2000-S, fluorescence microscope (Nikon, Melville, NY). The number of positive cells (green fluorescence) in four fields of each slide was counted under ×200 magnification. Images were captured and merged using Nikon Advanced Research Imaging Software available at the Meharry Morphology Core (supported by U.S. National Institutes of Health grant U54NS041071).
PY - 2010/10
Y1 - 2010/10
N2 - Objective: To investigate the effects of epigallocatechin gallate (EGCG), an extract of green tea on cultured human leiomyoma cells (HuLM). Design: Laboratory study. Setting: University hospitals. Patient(s): Not applicable. Intervention(s): Not applicable. Main Outcome Measure(s): The HuLM cells were treated with various EGCG concentrations. Cell proliferation was assayed using Hoechst 33258 dye, and apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Total RNA was isolated, and gene expression profiling was performed on 84 key genes related to 18 different signal transduction pathways. The protein levels of PCNA, CDK4, BCL2, and BAX were examined by Western blot analysis. Result(s): The HuLM cells treated with EGCG showed a dose-dependent and time-dependent inhibition of cell proliferation. The TUNEL staining indicated a significant increase in apoptosis in HuLM cells treated with 100 μM of EGCG compared with untreated control. Gene expression profiling indicated that EGCG treatment up-regulated representative genes from the transforming growth factor β (TGF-β) and stress pathways, while inhibiting the survival pathway and NFκB-dependent inflammatory pathway. Western blot analysis confirmed that EGCG at ≥50 μM significantly decreased the expression of PCNA, CDK4, and BCL2 as well as increased the expression of the proapoptotic BAX in a dose-dependent manner. Conclusion(s): Epigallocatechin gallate inhibits the proliferation of HuLM cells and induces apoptosis. These results suggest that EGCG may be a potential anti-uterine fibroid agent acting through multiple signal transduction pathways.
AB - Objective: To investigate the effects of epigallocatechin gallate (EGCG), an extract of green tea on cultured human leiomyoma cells (HuLM). Design: Laboratory study. Setting: University hospitals. Patient(s): Not applicable. Intervention(s): Not applicable. Main Outcome Measure(s): The HuLM cells were treated with various EGCG concentrations. Cell proliferation was assayed using Hoechst 33258 dye, and apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Total RNA was isolated, and gene expression profiling was performed on 84 key genes related to 18 different signal transduction pathways. The protein levels of PCNA, CDK4, BCL2, and BAX were examined by Western blot analysis. Result(s): The HuLM cells treated with EGCG showed a dose-dependent and time-dependent inhibition of cell proliferation. The TUNEL staining indicated a significant increase in apoptosis in HuLM cells treated with 100 μM of EGCG compared with untreated control. Gene expression profiling indicated that EGCG treatment up-regulated representative genes from the transforming growth factor β (TGF-β) and stress pathways, while inhibiting the survival pathway and NFκB-dependent inflammatory pathway. Western blot analysis confirmed that EGCG at ≥50 μM significantly decreased the expression of PCNA, CDK4, and BCL2 as well as increased the expression of the proapoptotic BAX in a dose-dependent manner. Conclusion(s): Epigallocatechin gallate inhibits the proliferation of HuLM cells and induces apoptosis. These results suggest that EGCG may be a potential anti-uterine fibroid agent acting through multiple signal transduction pathways.
KW - Apoptosis
KW - epigallocatechin gallate
KW - leiomyoma
KW - proliferation
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U2 - 10.1016/j.fertnstert.2009.08.065
DO - 10.1016/j.fertnstert.2009.08.065
M3 - Article
C2 - 19819432
AN - SCOPUS:77957205288
SN - 0015-0282
VL - 94
SP - 1887
EP - 1893
JO - Fertility and sterility
JF - Fertility and sterility
IS - 5
ER -