Apoptosis and proliferation of human peritoneal fibroblasts in response to hypoxia

Ghassan M. Saed, Michael P. Diamond

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Objective: To determine the effect of hypoxia on apoptosis and proliferation of fibroblasts cultured from normal peritoneum or adhesions. Design: Prospective experimental study. Setting: University medical center. Patient(s): Primary cultures of fibroblasts established from peritoneal and adhesion tissues of the same patients. Intervention(s): Hypoxia treatment of the primary cultured fibroblasts. Main Outcome Measure(s): Apoptosis was assessed by the TUNEL assay and by the BCL-2-BAX expression ratio, as determined using multiplex reverse transcription polymerase chain reaction. The proliferation rate of these cells was determined by measuring the proliferative fraction using the flow cytometry in adhesion and peritoneal fibroblasts under normal and hypoxic conditions. Result(s): Compared with fibroblasts from normal peritoneum, fibroblasts from adhesions had significant complementary decreases in apoptosis and an increase in proliferation. In response to hypoxia, the Bcl-2-bax mRNA ratio was significantly higher in fibroblasts from adhesions as compared with fibroblasts from normal peritoneum. The degree of apoptosis induced by hypoxia correlated with the increase in the bcl-2-bax ratio in fibroblasts derived from both normal peritoneum and adhesions. Conclusion(s): Hypoxia induces proliferation while inhibiting apoptosis in fibroblasts from adhesion, thereby creating a phenotype that would favor adhesion development.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalFertility and Sterility
Volume78
Issue number1
DOIs
StatePublished - Jul 15 2002
Externally publishedYes

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Keywords

  • Adhesion
  • Fibroblasts
  • Hypoxia
  • Multiplex RT-PCR
  • Peritoneum

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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