Apoptosis in the retina during MCMV retinitis in immunosuppressed BALB/c mice

Ming Zhang, Sally S. Atherton

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Cytomegalovirus (CMV) retinitis is the most common opportunistic ocular infection observed in immunosuppressed (IS) adult and pediatric patients. Due to the species restriction of the cytomegaloviruses, mice infected with murine CMV (MCMV) have been used to study the pathogenesis of CMV retinitis. Objectives: The objectives of this study were to determine if retinal glial cells are the targets of MCMV infection and to determine which cells in the retina become apoptotic following inoculation of MCMV via the supraciliary route. Study design: Adult female BALB/c mice were IS with methylprednisolone; one half of the mice were injected with MCMV and one half of the mice were injected with an equivalent volume of tissue culture medium via the supraciliary route. Animals were sacrificed and frozen sections of eyes were stained for MCMV early antigen, RPE65, CD45 or TUNEL; additional slides were double stained with combinations of the above reagents. Results and conclusions: The results indicate that most apoptotic cells in the retina were not virus infected, most apoptotic cells were not infiltrating CD45 positive leukocytes, and retinal glial cells were infected with MCMV but only late in infection. Together, these results suggest that retinal cells that undergo apoptosis during MCMV infection are neurons and that apoptosis of uninfected bystander cells is an important component of the pathogenesis of CMV retinitis.

Original languageEnglish (US)
Pages (from-to)137-147
Number of pages11
JournalJournal of Clinical Virology
Volume25
Issue numberSUPPL. 2
StatePublished - Jan 1 2002

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Retinitis
Retina
Cytomegalovirus Retinitis
Apoptosis
Muromegalovirus
Neuroglia
Infection
CD45 Antigens
Eye Infections
Opportunistic Infections
Methylprednisolone
In Situ Nick-End Labeling
Frozen Sections
Culture Media
Leukocytes
Pediatrics
Viruses
Neurons

Keywords

  • Apoptosis
  • Immunosuppression
  • Mouse
  • Murine cytomegalovirus
  • Ocular infection
  • Retinitis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Apoptosis in the retina during MCMV retinitis in immunosuppressed BALB/c mice. / Zhang, Ming; Atherton, Sally S.

In: Journal of Clinical Virology, Vol. 25, No. SUPPL. 2, 01.01.2002, p. 137-147.

Research output: Contribution to journalArticle

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