Apoptosis in the skeletal muscle of untreated children with juvenile dermatomyositis

Impact of duration of untreated disease

Yongdong Zhao, Tamara O. Fedczyna, Violet McVicker, Jan Caliendo, Honglin Li, Lauren M. Pachman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Juvenile dermatomyositis (JDM) is the most common myopathy in children with characteristic skin rash and muscle weakness, in which longer duration of untreated disease was associated with less muscle weakness. The duration of untreated inflammation may alter the apoptotic pathways involved in skeletal muscle damage. Diagnostic muscle biopsies from 14 untreated patients were stained for apoptosis markers. TUNEL-positive nuclei and caspase 3 were detected within the laminin layer, indicating apoptosis of skeletal muscle nuclei. Untreated JDM disease duration greater than 2 months ("long"), was associated with higher Fas-positive cell counts in the perivascular region compared with the "short" disease duration group, 2 months or less. Within the "long" duration group, higher Fas-positive cell counts were positively associated with increased TUNEL-positive nuclei and caspase 3. We conclude that the duration of untreated disease (chronic inflammation) influences the mode of continuing cell damage and death in children with JDM.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalClinical Immunology
Volume125
Issue number2
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

Fingerprint

Skeletal Muscle
Muscle Weakness
In Situ Nick-End Labeling
Apoptosis
Caspase 3
Cell Count
Inflammation
Laminin
Muscular Diseases
Exanthema
Chronic Disease
Cell Death
Biopsy
Muscles
Juvenile dermatomyositis

Keywords

  • Apoptosis
  • Disease duration
  • Juvenile dermatomyositis (JDM)
  • Skeletal muscle

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Apoptosis in the skeletal muscle of untreated children with juvenile dermatomyositis : Impact of duration of untreated disease. / Zhao, Yongdong; Fedczyna, Tamara O.; McVicker, Violet; Caliendo, Jan; Li, Honglin; Pachman, Lauren M.

In: Clinical Immunology, Vol. 125, No. 2, 01.11.2007, p. 165-172.

Research output: Contribution to journalArticle

Zhao, Yongdong ; Fedczyna, Tamara O. ; McVicker, Violet ; Caliendo, Jan ; Li, Honglin ; Pachman, Lauren M. / Apoptosis in the skeletal muscle of untreated children with juvenile dermatomyositis : Impact of duration of untreated disease. In: Clinical Immunology. 2007 ; Vol. 125, No. 2. pp. 165-172.
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