Apoptosis induction by 4β-acetoxyscirpendiol from Paecilomyces tenuipes in human leukaemia cell lines

H. C. Han, U. Lindequist, J. W. Hyun, Y. H. Kim, H. S. An, D. H. Lee, Ha Won Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The carpophores of Paecilomyces tenuipes are known in the Orient for their strong antitumor activity. In continuation of our study on acetoxyscirpendiol (ASD, 4β-acetoxyscirpene-3α,15-diol) as a cytotoxic component from this fungus, we report particularly on the mode of action of ASD in inducing apoptosis in human MOLT-4, THP-1 and Jurkat T cell leukaemia in vitro. The antiproliferative effects of ASD seem attributable to its induction of apoptosis in the cells, as it blocked the cell cycle, induced hypodiploidity and bound annexin V and also cleaved poly-(ADP-ribose) polymerase (PARP) in these cell lines. The 50% inhibitory concentrations (IC 50 ) of ASD on MOLT-4, THP-1 and Jurkat T cells were found to be 60, 85 and 60 ng/ml, respectively. ASD arrested the cell cycle at the G 1 /S transition and showed hypodiploidity due to the accumulation of sub-G 0 population. Annexin V binding was increased in the presence of ASD in the MOLT-4 cell line in a time-dependent manner. ASD and three of its derivatives also induced cleavage of PARP in both MOLT-4 and Jurkat T cell lines. From these data, it is suggested that ASD exerts its cytotoxic activity by inducing apoptosis in leukaemia cell lines in vitro.

Original languageEnglish (US)
Pages (from-to)42-49
Number of pages8
JournalPharmazie
Volume59
Issue number1
StatePublished - Jan 1 2004
Externally publishedYes

Fingerprint

Paecilomyces
Leukemia
Apoptosis
Cell Line
Jurkat Cells
Poly(ADP-ribose) Polymerases
Annexin A5
Cell Cycle
T-Lymphocytes
T-Cell Leukemia
4-acetoxyscirpene-3,15-diol
Inhibitory Concentration 50
Fungi

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Han, H. C., Lindequist, U., Hyun, J. W., Kim, Y. H., An, H. S., Lee, D. H., & Kim, H. W. (2004). Apoptosis induction by 4β-acetoxyscirpendiol from Paecilomyces tenuipes in human leukaemia cell lines. Pharmazie, 59(1), 42-49.

Apoptosis induction by 4β-acetoxyscirpendiol from Paecilomyces tenuipes in human leukaemia cell lines. / Han, H. C.; Lindequist, U.; Hyun, J. W.; Kim, Y. H.; An, H. S.; Lee, D. H.; Kim, Ha Won.

In: Pharmazie, Vol. 59, No. 1, 01.01.2004, p. 42-49.

Research output: Contribution to journalArticle

Han, HC, Lindequist, U, Hyun, JW, Kim, YH, An, HS, Lee, DH & Kim, HW 2004, 'Apoptosis induction by 4β-acetoxyscirpendiol from Paecilomyces tenuipes in human leukaemia cell lines', Pharmazie, vol. 59, no. 1, pp. 42-49.
Han HC, Lindequist U, Hyun JW, Kim YH, An HS, Lee DH et al. Apoptosis induction by 4β-acetoxyscirpendiol from Paecilomyces tenuipes in human leukaemia cell lines. Pharmazie. 2004 Jan 1;59(1):42-49.
Han, H. C. ; Lindequist, U. ; Hyun, J. W. ; Kim, Y. H. ; An, H. S. ; Lee, D. H. ; Kim, Ha Won. / Apoptosis induction by 4β-acetoxyscirpendiol from Paecilomyces tenuipes in human leukaemia cell lines. In: Pharmazie. 2004 ; Vol. 59, No. 1. pp. 42-49.
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