Apoptosis is associated with cleavage of a 5 kDa fragment from RB which mimics dephosphorylation and modulates E2F binding

Wei Dong Chen, Gregory A. Otterson, Stan Lipkowitz, Samir N. Khleif, Amy B. Coxon, Frederic J. Kaye

Research output: Contribution to journalArticle

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Abstract

Dephosphorylation of the RB protein has been reported to be associated with apoptosis. In contrast, we show that treatment of HL60 cells with etoposide or cytosine arabinoside or treatment of breast epithelial cells with α-FAS is associated with the cleavage of a 5 kDa fragment from the C-terminus of RB, resulting in a truncated product that we have designated as p100cl. This cleavage event coincides with the activation of cysteine proteases at the onset of apoptosis, is blocked by the addition of iodoacetamide to cells prior to the onset of apoptosis, and results in the expression of faster migrating protein species which can mimic dephosphorylated RB. The free 5 kDa fragment is detected only during apoptosis, predicts a cleavage site that we have mapped to a unique CPP32-like recognition sequence which is present at the C-terminus of all reported RB homologues, and results in a truncated RB protein with enhanced E2F binding affinity. While the causality for this cleavage event in the apoptotic process is still under investigation, our findings suggest distinct post-translational pathways for the RB product between cells examined during growth arrest (p105 hypophosphorylated RB) or apoptosis (p100cl).

Original languageEnglish (US)
Pages (from-to)1243-1248
Number of pages6
JournalOncogene
Volume14
Issue number10
DOIs
StatePublished - Jan 1 1997

Fingerprint

Apoptosis
Iodoacetamide
Proteins
Cysteine Proteases
HL-60 Cells
Cytarabine
Etoposide
Causality
Breast
Epithelial Cells
Growth

Keywords

  • Apoptosis
  • Chemotherapy
  • Cysteine protease
  • FAS
  • Retinoblastoma protein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Chen, W. D., Otterson, G. A., Lipkowitz, S., Khleif, S. N., Coxon, A. B., & Kaye, F. J. (1997). Apoptosis is associated with cleavage of a 5 kDa fragment from RB which mimics dephosphorylation and modulates E2F binding. Oncogene, 14(10), 1243-1248. https://doi.org/10.1038/sj.onc.1201096

Apoptosis is associated with cleavage of a 5 kDa fragment from RB which mimics dephosphorylation and modulates E2F binding. / Chen, Wei Dong; Otterson, Gregory A.; Lipkowitz, Stan; Khleif, Samir N.; Coxon, Amy B.; Kaye, Frederic J.

In: Oncogene, Vol. 14, No. 10, 01.01.1997, p. 1243-1248.

Research output: Contribution to journalArticle

Chen, WD, Otterson, GA, Lipkowitz, S, Khleif, SN, Coxon, AB & Kaye, FJ 1997, 'Apoptosis is associated with cleavage of a 5 kDa fragment from RB which mimics dephosphorylation and modulates E2F binding', Oncogene, vol. 14, no. 10, pp. 1243-1248. https://doi.org/10.1038/sj.onc.1201096
Chen, Wei Dong ; Otterson, Gregory A. ; Lipkowitz, Stan ; Khleif, Samir N. ; Coxon, Amy B. ; Kaye, Frederic J. / Apoptosis is associated with cleavage of a 5 kDa fragment from RB which mimics dephosphorylation and modulates E2F binding. In: Oncogene. 1997 ; Vol. 14, No. 10. pp. 1243-1248.
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