Apoptosis de fibrolastos gingivales en periodontitis

Translated title of the contribution: Apoptosis of gingival fibroblasts in periodontitis

Roger Mauricio Arce Munoz, Oscar Tamayo, Armando Cortés

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Human gingival fibroblasts (HGF) have an important role in the periodontal immune response. The fibroblasts alter their normal behavior in response to pro-inflammatory cytokines. It is believed that HGF can be diminished and/or eliminated by means of apoptosis. The purpose of this study was to determine and to quantify apoptosis of HGF in periodontium biopsies from healthy and chronic periodontitis patients. Methods: A clinical cross-sectional study in people with healthy periodontium (S), gingivitis (G) and chronic periodontitis (PC) patients was carried out. The periodontal biopsies were obtained and inummostained by means of: hematoxylin-eosin, caspase-3, vimentin and caspase-vimentin double-staining for specific visualization of apoptotic fibroblasts. Histopathological and digital analyses were performed. Descriptive statistics were applied to categorical and nominal variobles. Results: Total cell population of HGF had an average of 430±67.6 cells/field in healthy people, and a significantly progressive decrease in gingivitis (270±37.1) and chronic periodontitis groups (206.5±69.8) (p<0.05). As for total population of inflammatory cells, an increase was noticed in gingivitis (191.8±50.1) and a decrease in periodontitis (109.3±21.7) without statistical significance. The expression of apoptotic HGF per field increased accordingly to the severity of the disease [28±16 in health (6.5%); 31±17 in gingivitis (11.5%) and 51±24 in periodontitis (24.8%),p<0.001]. Similar findings were observed for inflammatory cells with different percentage expression [17±13 in health (23%); 28±19 in gingivitis (14.6%) and 47±35 in periodontitis (43.1%), p<0.05]. The relationship between the percentage of expression of apoptotic cells and probing pocket depth was proportional but not significant (p>0.5, r2=0.02); while for the inflammatory cells a significant relationship was observed (p<0.05, r2=0.2018). Conclusions: The results in this report conclude that HGF and inflammatory cells show apoptosis (caspase-3 expression), and apoptotic cells are significantly increased in gingivitis and chronic periodontitis groups.

Original languageSpanish
Pages (from-to)197-209
Number of pages13
JournalColombia Medica
Volume38
Issue number3
StatePublished - Jul 1 2007

Fingerprint

Periodontitis
Fibroblasts
Chronic Periodontitis
Apoptosis
Gingivitis
Periodontium
Vimentin
Caspase 3
Biopsy
Hematoxylin
Eosine Yellowish-(YS)
Caspases
Cross-Sectional Studies
Staining and Labeling
Cytokines
Population

Keywords

  • Apoptosis
  • Fibroblasts
  • Gingival
  • Gingivitis
  • Immunohistochemistry
  • Pathogenesis
  • Periodontitis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Arce Munoz, R. M., Tamayo, O., & Cortés, A. (2007). Apoptosis de fibrolastos gingivales en periodontitis. Colombia Medica, 38(3), 197-209.

Apoptosis de fibrolastos gingivales en periodontitis. / Arce Munoz, Roger Mauricio; Tamayo, Oscar; Cortés, Armando.

In: Colombia Medica, Vol. 38, No. 3, 01.07.2007, p. 197-209.

Research output: Contribution to journalArticle

Arce Munoz, RM, Tamayo, O & Cortés, A 2007, 'Apoptosis de fibrolastos gingivales en periodontitis', Colombia Medica, vol. 38, no. 3, pp. 197-209.
Arce Munoz, Roger Mauricio ; Tamayo, Oscar ; Cortés, Armando. / Apoptosis de fibrolastos gingivales en periodontitis. In: Colombia Medica. 2007 ; Vol. 38, No. 3. pp. 197-209.
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N2 - Introduction: Human gingival fibroblasts (HGF) have an important role in the periodontal immune response. The fibroblasts alter their normal behavior in response to pro-inflammatory cytokines. It is believed that HGF can be diminished and/or eliminated by means of apoptosis. The purpose of this study was to determine and to quantify apoptosis of HGF in periodontium biopsies from healthy and chronic periodontitis patients. Methods: A clinical cross-sectional study in people with healthy periodontium (S), gingivitis (G) and chronic periodontitis (PC) patients was carried out. The periodontal biopsies were obtained and inummostained by means of: hematoxylin-eosin, caspase-3, vimentin and caspase-vimentin double-staining for specific visualization of apoptotic fibroblasts. Histopathological and digital analyses were performed. Descriptive statistics were applied to categorical and nominal variobles. Results: Total cell population of HGF had an average of 430±67.6 cells/field in healthy people, and a significantly progressive decrease in gingivitis (270±37.1) and chronic periodontitis groups (206.5±69.8) (p<0.05). As for total population of inflammatory cells, an increase was noticed in gingivitis (191.8±50.1) and a decrease in periodontitis (109.3±21.7) without statistical significance. The expression of apoptotic HGF per field increased accordingly to the severity of the disease [28±16 in health (6.5%); 31±17 in gingivitis (11.5%) and 51±24 in periodontitis (24.8%),p<0.001]. Similar findings were observed for inflammatory cells with different percentage expression [17±13 in health (23%); 28±19 in gingivitis (14.6%) and 47±35 in periodontitis (43.1%), p<0.05]. The relationship between the percentage of expression of apoptotic cells and probing pocket depth was proportional but not significant (p>0.5, r2=0.02); while for the inflammatory cells a significant relationship was observed (p<0.05, r2=0.2018). Conclusions: The results in this report conclude that HGF and inflammatory cells show apoptosis (caspase-3 expression), and apoptotic cells are significantly increased in gingivitis and chronic periodontitis groups.

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