APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress

Jin Xiu Pan, Fulei Tang, Fei Xiong, Lei Xiong, Peng Zeng, Bo Wang, Kai Zhao, Haohan Guo, Cui Shun, Wen Fang Xia, Lin Mei, Wen Cheng Xiong

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Amyloid precursor protein (APP) is ubiquitously expressed in various types of cells including bone cells. Mutations in App gene result in early-onset Alzheimer’s disease (AD). However, little is known about its physiological function in bone homeostasis. Here, we provide evidence for APP’s role in promoting bone formation. Mice that knocked out App gene (APP −/− ) exhibit osteoporotic-like deficit, including reduced trabecular and cortical bone mass. Such a deficit is likely due in large to a decrease in osteoblast (OB)-mediated bone formation, as little change in bone resorption was detected in the mutant mice. Further mechanical studies of APP −/− OBs showed an impairment in mitochondrial function, accompanied with increased reactive oxygen species (ROS) and apoptosis. Intriguingly, these deficits, resemble to those in Tg2576 animal model of AD that expresses Swedish mutant APP (APPswe), were diminished by treatment with an anti-oxidant NAC (n-acetyl-l-cysteine), uncovering ROS as a critical underlying mechanism. Taken together, these results identify an unrecognized physiological function of APP in promoting OB survival and bone formation, implicate APPswe acting as a dominant negative factor, and reveal a potential clinical value of NAC in treatment of AD-associated osteoporotic deficits.

Original languageEnglish (US)
Article number1077
JournalCell Death and Disease
Volume9
Issue number11
DOIs
StatePublished - Nov 1 2018
Externally publishedYes

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Amyloid beta-Protein Precursor
Osteoblasts
Osteogenesis
Oxidative Stress
Alzheimer Disease
Cysteine
Reactive Oxygen Species
Bone and Bones
Mutant Proteins
Bone Resorption
Amyloid
Oxidants
Genes
Homeostasis
Animal Models
Apoptosis
Mutation

ASJC Scopus subject areas

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Cite this

APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress. / Pan, Jin Xiu; Tang, Fulei; Xiong, Fei; Xiong, Lei; Zeng, Peng; Wang, Bo; Zhao, Kai; Guo, Haohan; Shun, Cui; Xia, Wen Fang; Mei, Lin; Xiong, Wen Cheng.

In: Cell Death and Disease, Vol. 9, No. 11, 1077, 01.11.2018.

Research output: Contribution to journalArticle

Pan, JX, Tang, F, Xiong, F, Xiong, L, Zeng, P, Wang, B, Zhao, K, Guo, H, Shun, C, Xia, WF, Mei, L & Xiong, WC 2018, 'APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress', Cell Death and Disease, vol. 9, no. 11, 1077. https://doi.org/10.1038/s41419-018-1123-7
Pan, Jin Xiu ; Tang, Fulei ; Xiong, Fei ; Xiong, Lei ; Zeng, Peng ; Wang, Bo ; Zhao, Kai ; Guo, Haohan ; Shun, Cui ; Xia, Wen Fang ; Mei, Lin ; Xiong, Wen Cheng. / APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress. In: Cell Death and Disease. 2018 ; Vol. 9, No. 11.
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