Abstract
Three new spectrophotometric methods for the determination of amlodipine besylate have been proposed. The first two methods, i.e. A and B, are based on the oxidation of the drug with Fe(III) and the estimation of Fe(II) produced after chelation with either 1,10-phenanthroline or 2,2′-bipyridyl at 500 and 515 nm, respectively. The Beer's law was obeyed in the concentration ranges of 2-10 and 4-14 μg ml -1 with molar absorptivity of 2.9 × 10 4 and 2.7 × 10 4 l mol -1 cm -1 for methods A and B, respectively. The third procedure depends on the interaction of amlodipine besylate with ammonium heptamolybdate tetrahydrate, which resulted in the formation of molybdenum blue (λ max 825 nm). The linear dynamic range and the molar absorptivity values were found to be 15-59 μg ml -1 and 1.8 × 10 4 l mol -1 cm -1, respectively. The results of the proposed procedures were validated statistically and compared with those obtained by the reference method. The proposed methods were applied successfully to the determination of amlodipine besylate in commercial tablets.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 913-919 |
| Number of pages | 7 |
| Journal | Farmaco |
| Volume | 59 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1 2004 |
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Keywords
- Amlodipine besylate
- Oxidation
- Pharmaceutical analysis
- Visible spectrophotometry
ASJC Scopus subject areas
- Pharmaceutical Science
- Drug Discovery
Cite this
Application of oxidants to the spectrophotometric determination of amlodipine besylate in pharmaceutical formulations. / Rahman, Nafisur; Singh, Manisha; Hoda, MD Nasrul.
In: Farmaco, Vol. 59, No. 11, 01.11.2004, p. 913-919.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of oxidants to the spectrophotometric determination of amlodipine besylate in pharmaceutical formulations
AU - Rahman, Nafisur
AU - Singh, Manisha
AU - Hoda, MD Nasrul
PY - 2004/11/1
Y1 - 2004/11/1
N2 - Three new spectrophotometric methods for the determination of amlodipine besylate have been proposed. The first two methods, i.e. A and B, are based on the oxidation of the drug with Fe(III) and the estimation of Fe(II) produced after chelation with either 1,10-phenanthroline or 2,2′-bipyridyl at 500 and 515 nm, respectively. The Beer's law was obeyed in the concentration ranges of 2-10 and 4-14 μg ml -1 with molar absorptivity of 2.9 × 10 4 and 2.7 × 10 4 l mol -1 cm -1 for methods A and B, respectively. The third procedure depends on the interaction of amlodipine besylate with ammonium heptamolybdate tetrahydrate, which resulted in the formation of molybdenum blue (λ max 825 nm). The linear dynamic range and the molar absorptivity values were found to be 15-59 μg ml -1 and 1.8 × 10 4 l mol -1 cm -1, respectively. The results of the proposed procedures were validated statistically and compared with those obtained by the reference method. The proposed methods were applied successfully to the determination of amlodipine besylate in commercial tablets.
AB - Three new spectrophotometric methods for the determination of amlodipine besylate have been proposed. The first two methods, i.e. A and B, are based on the oxidation of the drug with Fe(III) and the estimation of Fe(II) produced after chelation with either 1,10-phenanthroline or 2,2′-bipyridyl at 500 and 515 nm, respectively. The Beer's law was obeyed in the concentration ranges of 2-10 and 4-14 μg ml -1 with molar absorptivity of 2.9 × 10 4 and 2.7 × 10 4 l mol -1 cm -1 for methods A and B, respectively. The third procedure depends on the interaction of amlodipine besylate with ammonium heptamolybdate tetrahydrate, which resulted in the formation of molybdenum blue (λ max 825 nm). The linear dynamic range and the molar absorptivity values were found to be 15-59 μg ml -1 and 1.8 × 10 4 l mol -1 cm -1, respectively. The results of the proposed procedures were validated statistically and compared with those obtained by the reference method. The proposed methods were applied successfully to the determination of amlodipine besylate in commercial tablets.
KW - Amlodipine besylate
KW - Oxidation
KW - Pharmaceutical analysis
KW - Visible spectrophotometry
UR - http://www.scopus.com/inward/record.url?scp=8444224703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=8444224703&partnerID=8YFLogxK
U2 - 10.1016/j.farmac.2004.07.009
DO - 10.1016/j.farmac.2004.07.009
M3 - Article
C2 - 15544797
AN - SCOPUS:8444224703
VL - 59
SP - 913
EP - 919
JO - ChemMedChem
JF - ChemMedChem
SN - 1860-7179
IS - 11
ER -