Application of oxidants to the spectrophotometric determination of amlodipine besylate in pharmaceutical formulations

Nafisur Rahman, Manisha Singh, MD Nasrul Hoda

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Three new spectrophotometric methods for the determination of amlodipine besylate have been proposed. The first two methods, i.e. A and B, are based on the oxidation of the drug with Fe(III) and the estimation of Fe(II) produced after chelation with either 1,10-phenanthroline or 2,2′-bipyridyl at 500 and 515 nm, respectively. The Beer's law was obeyed in the concentration ranges of 2-10 and 4-14 μg ml -1 with molar absorptivity of 2.9 × 10 4 and 2.7 × 10 4 l mol -1 cm -1 for methods A and B, respectively. The third procedure depends on the interaction of amlodipine besylate with ammonium heptamolybdate tetrahydrate, which resulted in the formation of molybdenum blue (λ max 825 nm). The linear dynamic range and the molar absorptivity values were found to be 15-59 μg ml -1 and 1.8 × 10 4 l mol -1 cm -1, respectively. The results of the proposed procedures were validated statistically and compared with those obtained by the reference method. The proposed methods were applied successfully to the determination of amlodipine besylate in commercial tablets.

Original languageEnglish (US)
Pages (from-to)913-919
Number of pages7
JournalFarmaco
Volume59
Issue number11
DOIs
StatePublished - Nov 2004

Keywords

  • Amlodipine besylate
  • Oxidation
  • Pharmaceutical analysis
  • Visible spectrophotometry

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery

Fingerprint Dive into the research topics of 'Application of oxidants to the spectrophotometric determination of amlodipine besylate in pharmaceutical formulations'. Together they form a unique fingerprint.

  • Cite this