Application of random amplified polymorphic DNA PCR for genomic analysis of HIV-1-infected individuals

Felix O. Aikhionbare, Cheryl L Newman, Chad Womack, William Roth, Ketan Shah, Vincent C. Bond

Research output: Contribution to journalArticle

Abstract

Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) is a DNA fingerprinting technique used to detect genomic polymorphisms. We employed sixteen different RAPD-PCR 10-mer primers to amplify DNA from the peripheral blood mononuclear cells (PBMC) of 80 HIV-1-infected individuals. These individuals were previously identified as either heterozygotes (+/Δ32) and homozygotes (+/+) for the CCR5 locus by PCR with gene specific primers. Four of the sixteen randomly selected RAPD primers produced distinguishable banding profiles between CCR5 (+/Δ32) heterozygotes and CCR5 (+/+) homozygotes. Direct sequencing of some RAPD-PCR products obtained with one of the four RAPD primers that were tested yielded clearly readable, but limited sequences, which were similar to portions of the previously published sequences for (+/+) homozygotes (98% similarity) and (+/Δ32) heterozygotes (87% similarity) of the CCR5 alleles. Thus, the RAPD-PCR technique may be useful for the identification of human molecular markers that may correlate with susceptibility to HIV-1-infection, or differences in disease progression among HIV-1-infected individuals.

Original languageEnglish (US)
Pages (from-to)25-31
Number of pages7
JournalMutation Research - Mutation Research Genomics
Volume406
Issue number1
DOIs
Publication statusPublished - Nov 1 1998

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Keywords

  • CCR5 gene
  • HIV-1
  • Polymorphism
  • RAPD-PCR

ASJC Scopus subject areas

  • Toxicology
  • Genetics

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