Application of response surface methodology to the modeling of α-amylase purification by aqueous two-phase systems

Wenbo Zhi, Jiangnan Song, Fan Ouyang, Jingxiu Bi

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Mathematical models concerning the purification of α-amylase from the cultivation supernatant of Bacillus subtilis in a polyethylene glycol-citrate aqueous two-phase system (ATP) are established with response surface methodology. The PEG3350, citrate and sodium chloride concentrations were selected as variables to evaluate the purification impact factors in aqueous two-phase system, including partition coefficients of α-amylase, total protein, purification factor and α-amylase yield. An experimental space with two-fold purification and over 90% yield of α-amylase is achieved through the optimized condition basing on the model. Two systems with low viscosity within said space were further selected to perform α-amylase purification and the experimental results coincide well with the calculation of the models, which indicates that the model provides a promising tool for experimental design of protein purification by aqueous two-phase system.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalJournal of Biotechnology
Volume118
Issue number2
DOIs
StatePublished - Aug 4 2005
Externally publishedYes

Fingerprint

Amylases
Purification
Citric Acid
Proteins
Bacillus subtilis
Sodium Chloride
Viscosity
Bacilli
Sodium chloride
Research Design
Theoretical Models
Design of experiments
Polyethylene glycols
Mathematical models

Keywords

  • Aqueous two-phase systems
  • Purification
  • Response surface methodology
  • α-Amylase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Application of response surface methodology to the modeling of α-amylase purification by aqueous two-phase systems. / Zhi, Wenbo; Song, Jiangnan; Ouyang, Fan; Bi, Jingxiu.

In: Journal of Biotechnology, Vol. 118, No. 2, 04.08.2005, p. 157-165.

Research output: Contribution to journalArticle

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