Application of spectral karyotyping to the analysis of the human chromosome complement of interspecies somatic cell hybrids

Sei ichi Matsui, Silviu L. Faitar, Michael R. Rossi, John Kenneth Cowell

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Mouse-human somatic cell hybrids have been extensively used in the molecular genetic dissection of human disease-related chromosome rearrangements because of their ability to selectively and randomly eliminate human chromosomes. This technology allows the isolation of structural chromosome abnormalities, which then allows determination of the precise molecular address of chromosome breakpoints associated with deletions and translocations, down to the nucleotides involved. The main confounding problem with the analysis of somatic cell hybrids is determining the exact chromosome complement unequivocally and quickly. Spectral karyotyping can identify each of the individual human chromosomes in a normal metaphase spread, as well as structural chromosome rearrangements - although, because of potential cross-hybridization between the human probe and mouse DNA sequences during the hybridization reaction, it has not been determined whether the same analysis will selectively identify human chromosomes on a mouse background. We show (to our knowledge, for the first time) that, under modified conditions of chromosomal in situ suppression hybridization, the standard spectral karyotyping probe does not cross-react with mouse chromosomes and can be used to identify subtle structurally rearranged chromosomes in hybrid cells. This analysis allows for the rapid and unequivocal identification of the human chromosome complement in these hybrids, as well as structural chromosome rearrangements that occur between mouse and human chromosomes that might otherwise confound the analysis.

Original languageEnglish (US)
Pages (from-to)30-35
Number of pages6
JournalCancer Genetics and Cytogenetics
Volume142
Issue number1
DOIs
StatePublished - Apr 1 2003

Fingerprint

Spectral Karyotyping
Hybrid Cells
Human Chromosomes
Chromosomes
Chromosome Breakpoints
Metaphase
Chromosome Aberrations
In Situ Hybridization
Dissection
Molecular Biology
Nucleotides
Technology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Application of spectral karyotyping to the analysis of the human chromosome complement of interspecies somatic cell hybrids. / Matsui, Sei ichi; Faitar, Silviu L.; Rossi, Michael R.; Cowell, John Kenneth.

In: Cancer Genetics and Cytogenetics, Vol. 142, No. 1, 01.04.2003, p. 30-35.

Research output: Contribution to journalArticle

@article{154eb579c92c49e1b113619e0345d85c,
title = "Application of spectral karyotyping to the analysis of the human chromosome complement of interspecies somatic cell hybrids",
abstract = "Mouse-human somatic cell hybrids have been extensively used in the molecular genetic dissection of human disease-related chromosome rearrangements because of their ability to selectively and randomly eliminate human chromosomes. This technology allows the isolation of structural chromosome abnormalities, which then allows determination of the precise molecular address of chromosome breakpoints associated with deletions and translocations, down to the nucleotides involved. The main confounding problem with the analysis of somatic cell hybrids is determining the exact chromosome complement unequivocally and quickly. Spectral karyotyping can identify each of the individual human chromosomes in a normal metaphase spread, as well as structural chromosome rearrangements - although, because of potential cross-hybridization between the human probe and mouse DNA sequences during the hybridization reaction, it has not been determined whether the same analysis will selectively identify human chromosomes on a mouse background. We show (to our knowledge, for the first time) that, under modified conditions of chromosomal in situ suppression hybridization, the standard spectral karyotyping probe does not cross-react with mouse chromosomes and can be used to identify subtle structurally rearranged chromosomes in hybrid cells. This analysis allows for the rapid and unequivocal identification of the human chromosome complement in these hybrids, as well as structural chromosome rearrangements that occur between mouse and human chromosomes that might otherwise confound the analysis.",
author = "Matsui, {Sei ichi} and Faitar, {Silviu L.} and Rossi, {Michael R.} and Cowell, {John Kenneth}",
year = "2003",
month = "4",
day = "1",
doi = "10.1016/S0165-4608(02)00730-6",
language = "English (US)",
volume = "142",
pages = "30--35",
journal = "Cancer Genetics and Cytogenetics",
issn = "0165-4608",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Application of spectral karyotyping to the analysis of the human chromosome complement of interspecies somatic cell hybrids

AU - Matsui, Sei ichi

AU - Faitar, Silviu L.

AU - Rossi, Michael R.

AU - Cowell, John Kenneth

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Mouse-human somatic cell hybrids have been extensively used in the molecular genetic dissection of human disease-related chromosome rearrangements because of their ability to selectively and randomly eliminate human chromosomes. This technology allows the isolation of structural chromosome abnormalities, which then allows determination of the precise molecular address of chromosome breakpoints associated with deletions and translocations, down to the nucleotides involved. The main confounding problem with the analysis of somatic cell hybrids is determining the exact chromosome complement unequivocally and quickly. Spectral karyotyping can identify each of the individual human chromosomes in a normal metaphase spread, as well as structural chromosome rearrangements - although, because of potential cross-hybridization between the human probe and mouse DNA sequences during the hybridization reaction, it has not been determined whether the same analysis will selectively identify human chromosomes on a mouse background. We show (to our knowledge, for the first time) that, under modified conditions of chromosomal in situ suppression hybridization, the standard spectral karyotyping probe does not cross-react with mouse chromosomes and can be used to identify subtle structurally rearranged chromosomes in hybrid cells. This analysis allows for the rapid and unequivocal identification of the human chromosome complement in these hybrids, as well as structural chromosome rearrangements that occur between mouse and human chromosomes that might otherwise confound the analysis.

AB - Mouse-human somatic cell hybrids have been extensively used in the molecular genetic dissection of human disease-related chromosome rearrangements because of their ability to selectively and randomly eliminate human chromosomes. This technology allows the isolation of structural chromosome abnormalities, which then allows determination of the precise molecular address of chromosome breakpoints associated with deletions and translocations, down to the nucleotides involved. The main confounding problem with the analysis of somatic cell hybrids is determining the exact chromosome complement unequivocally and quickly. Spectral karyotyping can identify each of the individual human chromosomes in a normal metaphase spread, as well as structural chromosome rearrangements - although, because of potential cross-hybridization between the human probe and mouse DNA sequences during the hybridization reaction, it has not been determined whether the same analysis will selectively identify human chromosomes on a mouse background. We show (to our knowledge, for the first time) that, under modified conditions of chromosomal in situ suppression hybridization, the standard spectral karyotyping probe does not cross-react with mouse chromosomes and can be used to identify subtle structurally rearranged chromosomes in hybrid cells. This analysis allows for the rapid and unequivocal identification of the human chromosome complement in these hybrids, as well as structural chromosome rearrangements that occur between mouse and human chromosomes that might otherwise confound the analysis.

UR - http://www.scopus.com/inward/record.url?scp=0037378177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037378177&partnerID=8YFLogxK

U2 - 10.1016/S0165-4608(02)00730-6

DO - 10.1016/S0165-4608(02)00730-6

M3 - Article

VL - 142

SP - 30

EP - 35

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

SN - 0165-4608

IS - 1

ER -