Application of unnatural amino acids to the de novo design of selective antibiotic peptides

Rickey Paige Hicks, Amanda L. Russell

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Citations (Scopus)

Abstract

Because of their unique mechanism of cytotoxicity against bacteria and other microorganisms, antimicrobial peptides have received a great deal of attention as possible therapeutic agents. Incorporation of unnatural amino acids into the peptide sequences has the potential to improve the organism selectivity and potency of these peptides as well as increase their metabolic stability. This protocol outlines the logic used to selectively incorporate unnatural amino acid into a peptide sequence in an attempt to obtain peptides with increased therapeutic potential as antibiotic agents.

Original languageEnglish (US)
Title of host publicationUnnatural Amino Acids
Subtitle of host publicationMethods and Protocols
EditorsLoredano Pollegioni, Stefano Servi
Pages135-167
Number of pages33
DOIs
StatePublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
Volume794
ISSN (Print)1064-3745

Fingerprint

Anti-Bacterial Agents
Amino Acids
Peptides
Bacteria
Therapeutics

Keywords

  • Antimicrobial peptides
  • De novo
  • Organism selectivity
  • Unnatural amino acids

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hicks, R. P., & Russell, A. L. (2012). Application of unnatural amino acids to the de novo design of selective antibiotic peptides. In L. Pollegioni, & S. Servi (Eds.), Unnatural Amino Acids: Methods and Protocols (pp. 135-167). (Methods in Molecular Biology; Vol. 794). https://doi.org/10.1007/978-1-61779-331-8_9

Application of unnatural amino acids to the de novo design of selective antibiotic peptides. / Hicks, Rickey Paige; Russell, Amanda L.

Unnatural Amino Acids: Methods and Protocols. ed. / Loredano Pollegioni; Stefano Servi. 2012. p. 135-167 (Methods in Molecular Biology; Vol. 794).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hicks, RP & Russell, AL 2012, Application of unnatural amino acids to the de novo design of selective antibiotic peptides. in L Pollegioni & S Servi (eds), Unnatural Amino Acids: Methods and Protocols. Methods in Molecular Biology, vol. 794, pp. 135-167. https://doi.org/10.1007/978-1-61779-331-8_9
Hicks RP, Russell AL. Application of unnatural amino acids to the de novo design of selective antibiotic peptides. In Pollegioni L, Servi S, editors, Unnatural Amino Acids: Methods and Protocols. 2012. p. 135-167. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-331-8_9
Hicks, Rickey Paige ; Russell, Amanda L. / Application of unnatural amino acids to the de novo design of selective antibiotic peptides. Unnatural Amino Acids: Methods and Protocols. editor / Loredano Pollegioni ; Stefano Servi. 2012. pp. 135-167 (Methods in Molecular Biology).
@inbook{6f0ba6e59e99449d9ff5c9903d22c65f,
title = "Application of unnatural amino acids to the de novo design of selective antibiotic peptides",
abstract = "Because of their unique mechanism of cytotoxicity against bacteria and other microorganisms, antimicrobial peptides have received a great deal of attention as possible therapeutic agents. Incorporation of unnatural amino acids into the peptide sequences has the potential to improve the organism selectivity and potency of these peptides as well as increase their metabolic stability. This protocol outlines the logic used to selectively incorporate unnatural amino acid into a peptide sequence in an attempt to obtain peptides with increased therapeutic potential as antibiotic agents.",
keywords = "Antimicrobial peptides, De novo, Organism selectivity, Unnatural amino acids",
author = "Hicks, {Rickey Paige} and Russell, {Amanda L.}",
year = "2012",
month = "1",
day = "1",
doi = "10.1007/978-1-61779-331-8_9",
language = "English (US)",
isbn = "9781617793301",
series = "Methods in Molecular Biology",
pages = "135--167",
editor = "Loredano Pollegioni and Stefano Servi",
booktitle = "Unnatural Amino Acids",

}

TY - CHAP

T1 - Application of unnatural amino acids to the de novo design of selective antibiotic peptides

AU - Hicks, Rickey Paige

AU - Russell, Amanda L.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Because of their unique mechanism of cytotoxicity against bacteria and other microorganisms, antimicrobial peptides have received a great deal of attention as possible therapeutic agents. Incorporation of unnatural amino acids into the peptide sequences has the potential to improve the organism selectivity and potency of these peptides as well as increase their metabolic stability. This protocol outlines the logic used to selectively incorporate unnatural amino acid into a peptide sequence in an attempt to obtain peptides with increased therapeutic potential as antibiotic agents.

AB - Because of their unique mechanism of cytotoxicity against bacteria and other microorganisms, antimicrobial peptides have received a great deal of attention as possible therapeutic agents. Incorporation of unnatural amino acids into the peptide sequences has the potential to improve the organism selectivity and potency of these peptides as well as increase their metabolic stability. This protocol outlines the logic used to selectively incorporate unnatural amino acid into a peptide sequence in an attempt to obtain peptides with increased therapeutic potential as antibiotic agents.

KW - Antimicrobial peptides

KW - De novo

KW - Organism selectivity

KW - Unnatural amino acids

UR - http://www.scopus.com/inward/record.url?scp=80655144847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80655144847&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-331-8_9

DO - 10.1007/978-1-61779-331-8_9

M3 - Chapter

C2 - 21956561

AN - SCOPUS:80655144847

SN - 9781617793301

T3 - Methods in Molecular Biology

SP - 135

EP - 167

BT - Unnatural Amino Acids

A2 - Pollegioni, Loredano

A2 - Servi, Stefano

ER -