Applications of CpG island microarrays for high-throughput analysis of DNA methylation

Pearlly S. Yan, Chuan Mu Chen, Huidong Shi, Farahnaz Rahmatpanah, Susan H. Wei, Tim Hui Ming Huang

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Differential methylation hybridization (DMH) is a high-throughput microarray technique designed to identify changes in DNA methylation patterns commonly observed in cancer and other disease states. The DMH methodology comprises three fundamental components: the arraying of CpG island clones on glass slides, the preparation of the sample amplicons under investigation, and the hybridization of amplicon targets onto the CpG island microarray. Herein, we outline the DMH protocol and illustrate its utility and the validation approaches used in analyzing the hypermethylation profile of breast tumor and normal samples.

Original languageEnglish (US)
Pages (from-to)2430S-2434S
JournalJournal of Nutrition
Volume132
Issue number8 SUPPL.
StatePublished - Aug 15 2002

Fingerprint

CpG Islands
DNA Methylation
Methylation
Glass
Clone Cells
Breast Neoplasms
Neoplasms

Keywords

  • CpG islands
  • DNA methylation
  • Microarray

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Yan, P. S., Chen, C. M., Shi, H., Rahmatpanah, F., Wei, S. H., & Huang, T. H. M. (2002). Applications of CpG island microarrays for high-throughput analysis of DNA methylation. Journal of Nutrition, 132(8 SUPPL.), 2430S-2434S.

Applications of CpG island microarrays for high-throughput analysis of DNA methylation. / Yan, Pearlly S.; Chen, Chuan Mu; Shi, Huidong; Rahmatpanah, Farahnaz; Wei, Susan H.; Huang, Tim Hui Ming.

In: Journal of Nutrition, Vol. 132, No. 8 SUPPL., 15.08.2002, p. 2430S-2434S.

Research output: Contribution to journalArticle

Yan, PS, Chen, CM, Shi, H, Rahmatpanah, F, Wei, SH & Huang, THM 2002, 'Applications of CpG island microarrays for high-throughput analysis of DNA methylation', Journal of Nutrition, vol. 132, no. 8 SUPPL., pp. 2430S-2434S.
Yan PS, Chen CM, Shi H, Rahmatpanah F, Wei SH, Huang THM. Applications of CpG island microarrays for high-throughput analysis of DNA methylation. Journal of Nutrition. 2002 Aug 15;132(8 SUPPL.):2430S-2434S.
Yan, Pearlly S. ; Chen, Chuan Mu ; Shi, Huidong ; Rahmatpanah, Farahnaz ; Wei, Susan H. ; Huang, Tim Hui Ming. / Applications of CpG island microarrays for high-throughput analysis of DNA methylation. In: Journal of Nutrition. 2002 ; Vol. 132, No. 8 SUPPL. pp. 2430S-2434S.
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