Aquaporin 1, Nox1, and Ask1 mediate oxidant-induced smooth muscle cell hypertrophy

Imad Al Ghouleh, Giovanna Frazziano, Andres I. Rodriguez, Gábor Csányi, Salony Maniar, Claudette M. St Croix, Eric E. Kelley, Loreto A. Egaña, Gyun Jee Song, Alessandro Bisello, Yong J. Lee, Patrick J. Pagano

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Aims Reactive oxygen species (ROS)-mediated intracellular signalling is well described in the vasculature, yet the precise roles of ROS in paracrine signalling are not known. Studies implicate interstitial ROS hydrogen peroxide (H2O2) in vascular disease, and plasma H2O 2 levels in the micromolar range are detectable in animal models and humans with hypertension. Recently, H2O2 was shown to cross biological membranes of non-vascular cells via aquaporin (Aqp) water channels. Previous findings suggest that H2O2 activates NADPH oxidase (Nox) enzymes in vascular cells and apoptosis signal-regulating kinase 1 (Ask1) in non-vascular cells. We hypothesized that extracellular H 2O2 induces smooth muscle cell (SMC) hypertrophy by a mechanism involving Aqp1, Nox1, and Ask1.Methods and resultsTreatment of rat aortic SMCs (rASMC) with exogenous H2O2 resulted in a concentration-dependent increase in Nox-derived superoxide (O2 •-), determined by L-012 chemiluminescence, cytochrome c and electron paramagnetic resonance. Nox1 was verified as the source of O 2·- by siRNA. Aqp1 siRNA attenuated H 2O2 cellular entry and H2O2-induced O2•- production. H2O2 treatment increased Ask1 activation and induced rASMC hypertrophy in a Nox1-dependent mechanism. Adenoviral-dominant-negative Ask1 attenuated H2O 2-induced rASMC hypertrophy and adenoviral overexpression of Ask1 augmented it.ConclusionOur results demonstrate for the first time that extracellular H2O2, at pathophysiological concentrations, stimulates rASMC Nox1-derived O2•-, subsequent Ask1 activation and SMC hypertrophy. The data demonstrate a novel pathway by which H2O2 enters vascular cells via aquaporins and activates Nox, leading to hypertrophy, and provide multiple novel targets for combinatorial therapeutics development targeting hypertrophy and vascular disease.

Original languageEnglish (US)
Pages (from-to)134-142
Number of pages9
JournalCardiovascular Research
Volume97
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

MAP Kinase Kinase Kinase 5
Aquaporin 1
Oxidants
Hypertrophy
Smooth Muscle Myocytes
Aquaporins
NADPH Oxidase
Reactive Oxygen Species
Vascular Diseases
Small Interfering RNA
Blood Vessels
L 012
Paracrine Communication
Electron Spin Resonance Spectroscopy
Luminescence
Cytochromes c
Superoxides
Hydrogen Peroxide
Animal Models
Hypertension

Keywords

  • Aquaporin
  • Ask1
  • Hydrogen peroxide
  • Hypertrophy
  • NADPH oxidase

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Al Ghouleh, I., Frazziano, G., Rodriguez, A. I., Csányi, G., Maniar, S., St Croix, C. M., ... Pagano, P. J. (2013). Aquaporin 1, Nox1, and Ask1 mediate oxidant-induced smooth muscle cell hypertrophy. Cardiovascular Research, 97(1), 134-142. https://doi.org/10.1093/cvr/cvs295

Aquaporin 1, Nox1, and Ask1 mediate oxidant-induced smooth muscle cell hypertrophy. / Al Ghouleh, Imad; Frazziano, Giovanna; Rodriguez, Andres I.; Csányi, Gábor; Maniar, Salony; St Croix, Claudette M.; Kelley, Eric E.; Egaña, Loreto A.; Song, Gyun Jee; Bisello, Alessandro; Lee, Yong J.; Pagano, Patrick J.

In: Cardiovascular Research, Vol. 97, No. 1, 01.01.2013, p. 134-142.

Research output: Contribution to journalArticle

Al Ghouleh, I, Frazziano, G, Rodriguez, AI, Csányi, G, Maniar, S, St Croix, CM, Kelley, EE, Egaña, LA, Song, GJ, Bisello, A, Lee, YJ & Pagano, PJ 2013, 'Aquaporin 1, Nox1, and Ask1 mediate oxidant-induced smooth muscle cell hypertrophy', Cardiovascular Research, vol. 97, no. 1, pp. 134-142. https://doi.org/10.1093/cvr/cvs295
Al Ghouleh I, Frazziano G, Rodriguez AI, Csányi G, Maniar S, St Croix CM et al. Aquaporin 1, Nox1, and Ask1 mediate oxidant-induced smooth muscle cell hypertrophy. Cardiovascular Research. 2013 Jan 1;97(1):134-142. https://doi.org/10.1093/cvr/cvs295
Al Ghouleh, Imad ; Frazziano, Giovanna ; Rodriguez, Andres I. ; Csányi, Gábor ; Maniar, Salony ; St Croix, Claudette M. ; Kelley, Eric E. ; Egaña, Loreto A. ; Song, Gyun Jee ; Bisello, Alessandro ; Lee, Yong J. ; Pagano, Patrick J. / Aquaporin 1, Nox1, and Ask1 mediate oxidant-induced smooth muscle cell hypertrophy. In: Cardiovascular Research. 2013 ; Vol. 97, No. 1. pp. 134-142.
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abstract = "Aims Reactive oxygen species (ROS)-mediated intracellular signalling is well described in the vasculature, yet the precise roles of ROS in paracrine signalling are not known. Studies implicate interstitial ROS hydrogen peroxide (H2O2) in vascular disease, and plasma H2O 2 levels in the micromolar range are detectable in animal models and humans with hypertension. Recently, H2O2 was shown to cross biological membranes of non-vascular cells via aquaporin (Aqp) water channels. Previous findings suggest that H2O2 activates NADPH oxidase (Nox) enzymes in vascular cells and apoptosis signal-regulating kinase 1 (Ask1) in non-vascular cells. We hypothesized that extracellular H 2O2 induces smooth muscle cell (SMC) hypertrophy by a mechanism involving Aqp1, Nox1, and Ask1.Methods and resultsTreatment of rat aortic SMCs (rASMC) with exogenous H2O2 resulted in a concentration-dependent increase in Nox-derived superoxide (O2 •-), determined by L-012 chemiluminescence, cytochrome c and electron paramagnetic resonance. Nox1 was verified as the source of O 2·- by siRNA. Aqp1 siRNA attenuated H 2O2 cellular entry and H2O2-induced O2•- production. H2O2 treatment increased Ask1 activation and induced rASMC hypertrophy in a Nox1-dependent mechanism. Adenoviral-dominant-negative Ask1 attenuated H2O 2-induced rASMC hypertrophy and adenoviral overexpression of Ask1 augmented it.ConclusionOur results demonstrate for the first time that extracellular H2O2, at pathophysiological concentrations, stimulates rASMC Nox1-derived O2•-, subsequent Ask1 activation and SMC hypertrophy. The data demonstrate a novel pathway by which H2O2 enters vascular cells via aquaporins and activates Nox, leading to hypertrophy, and provide multiple novel targets for combinatorial therapeutics development targeting hypertrophy and vascular disease.",
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T1 - Aquaporin 1, Nox1, and Ask1 mediate oxidant-induced smooth muscle cell hypertrophy

AU - Al Ghouleh, Imad

AU - Frazziano, Giovanna

AU - Rodriguez, Andres I.

AU - Csányi, Gábor

AU - Maniar, Salony

AU - St Croix, Claudette M.

AU - Kelley, Eric E.

AU - Egaña, Loreto A.

AU - Song, Gyun Jee

AU - Bisello, Alessandro

AU - Lee, Yong J.

AU - Pagano, Patrick J.

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N2 - Aims Reactive oxygen species (ROS)-mediated intracellular signalling is well described in the vasculature, yet the precise roles of ROS in paracrine signalling are not known. Studies implicate interstitial ROS hydrogen peroxide (H2O2) in vascular disease, and plasma H2O 2 levels in the micromolar range are detectable in animal models and humans with hypertension. Recently, H2O2 was shown to cross biological membranes of non-vascular cells via aquaporin (Aqp) water channels. Previous findings suggest that H2O2 activates NADPH oxidase (Nox) enzymes in vascular cells and apoptosis signal-regulating kinase 1 (Ask1) in non-vascular cells. We hypothesized that extracellular H 2O2 induces smooth muscle cell (SMC) hypertrophy by a mechanism involving Aqp1, Nox1, and Ask1.Methods and resultsTreatment of rat aortic SMCs (rASMC) with exogenous H2O2 resulted in a concentration-dependent increase in Nox-derived superoxide (O2 •-), determined by L-012 chemiluminescence, cytochrome c and electron paramagnetic resonance. Nox1 was verified as the source of O 2·- by siRNA. Aqp1 siRNA attenuated H 2O2 cellular entry and H2O2-induced O2•- production. H2O2 treatment increased Ask1 activation and induced rASMC hypertrophy in a Nox1-dependent mechanism. Adenoviral-dominant-negative Ask1 attenuated H2O 2-induced rASMC hypertrophy and adenoviral overexpression of Ask1 augmented it.ConclusionOur results demonstrate for the first time that extracellular H2O2, at pathophysiological concentrations, stimulates rASMC Nox1-derived O2•-, subsequent Ask1 activation and SMC hypertrophy. The data demonstrate a novel pathway by which H2O2 enters vascular cells via aquaporins and activates Nox, leading to hypertrophy, and provide multiple novel targets for combinatorial therapeutics development targeting hypertrophy and vascular disease.

AB - Aims Reactive oxygen species (ROS)-mediated intracellular signalling is well described in the vasculature, yet the precise roles of ROS in paracrine signalling are not known. Studies implicate interstitial ROS hydrogen peroxide (H2O2) in vascular disease, and plasma H2O 2 levels in the micromolar range are detectable in animal models and humans with hypertension. Recently, H2O2 was shown to cross biological membranes of non-vascular cells via aquaporin (Aqp) water channels. Previous findings suggest that H2O2 activates NADPH oxidase (Nox) enzymes in vascular cells and apoptosis signal-regulating kinase 1 (Ask1) in non-vascular cells. We hypothesized that extracellular H 2O2 induces smooth muscle cell (SMC) hypertrophy by a mechanism involving Aqp1, Nox1, and Ask1.Methods and resultsTreatment of rat aortic SMCs (rASMC) with exogenous H2O2 resulted in a concentration-dependent increase in Nox-derived superoxide (O2 •-), determined by L-012 chemiluminescence, cytochrome c and electron paramagnetic resonance. Nox1 was verified as the source of O 2·- by siRNA. Aqp1 siRNA attenuated H 2O2 cellular entry and H2O2-induced O2•- production. H2O2 treatment increased Ask1 activation and induced rASMC hypertrophy in a Nox1-dependent mechanism. Adenoviral-dominant-negative Ask1 attenuated H2O 2-induced rASMC hypertrophy and adenoviral overexpression of Ask1 augmented it.ConclusionOur results demonstrate for the first time that extracellular H2O2, at pathophysiological concentrations, stimulates rASMC Nox1-derived O2•-, subsequent Ask1 activation and SMC hypertrophy. The data demonstrate a novel pathway by which H2O2 enters vascular cells via aquaporins and activates Nox, leading to hypertrophy, and provide multiple novel targets for combinatorial therapeutics development targeting hypertrophy and vascular disease.

KW - Aquaporin

KW - Ask1

KW - Hydrogen peroxide

KW - Hypertrophy

KW - NADPH oxidase

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