Arginase from Candida albicans

Uma Gunasekaran, Elias Manavathu, Muthukumaran Gunasekaran

Research output: Contribution to journalArticle

Abstract

The enzyme arginase (EC 3.5.3.1.) catalyses the hydrolysis of L-arginine to equimolar amounts of L-ornithine and area. This enzyme was studied in Candida albicans, an opportunistic yeast which causes diseases ranging from superficial infections to the deep systemic disease, candidiasis, in immunosuppressed humans. The fungus was grown as yeast in synthetic medium containing L-arginine (pH 4.5) as the sole nitrogen source, at room temperature for various growth periods. The organism was also grown at pH 6.5 and at 37°C for pseudohyphal production. Arginase activity was measured from the cell-free homogenate. The maximal activity of arginase was at 12 h in the pseudohyphal phase and 72 h in the yeast phase. Among the different forms of nitrogen tested for arginase induction, arginine induced maximum arginase activity compared with others such as ammonia, glutamine and glutamate which induced comparatively less arginase activity. Saboraud dextrose broth, a complex medium, supported maximum growth of the organism, but tryptic soy broth supported maximum enzyme activity. The results also indicate that L-arginine is essential for arginase induction.

Original languageEnglish (US)
Pages (from-to)153-160
Number of pages8
JournalBiomedical Letters
Volume54
Issue number215
StatePublished - Dec 1 1996

Fingerprint

Arginase
Candida
Candida albicans
Arginine
Yeast
Yeasts
Nitrogen
Enzymes
Ornithine
Enzyme activity
Growth
Fungi
Glutamine
Ammonia
Glutamic Acid
Hydrolysis
Glucose
Temperature
Infection

Keywords

  • Arginase
  • Candida albicans
  • Dimorphism

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Gunasekaran, U., Manavathu, E., & Gunasekaran, M. (1996). Arginase from Candida albicans. Biomedical Letters, 54(215), 153-160.

Arginase from Candida albicans. / Gunasekaran, Uma; Manavathu, Elias; Gunasekaran, Muthukumaran.

In: Biomedical Letters, Vol. 54, No. 215, 01.12.1996, p. 153-160.

Research output: Contribution to journalArticle

Gunasekaran, U, Manavathu, E & Gunasekaran, M 1996, 'Arginase from Candida albicans', Biomedical Letters, vol. 54, no. 215, pp. 153-160.
Gunasekaran U, Manavathu E, Gunasekaran M. Arginase from Candida albicans. Biomedical Letters. 1996 Dec 1;54(215):153-160.
Gunasekaran, Uma ; Manavathu, Elias ; Gunasekaran, Muthukumaran. / Arginase from Candida albicans. In: Biomedical Letters. 1996 ; Vol. 54, No. 215. pp. 153-160.
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