Artificial human corneas: Scaffolds for transplantation and host regeneration

May Griffith, Malik Hakim, Shigeto Shimmura, Mitchell Aaron Watsky, Fengfu Li, David Carlsson, Charles J. Doillon, Masatsugu Nakamura, Erik Suuronen, Naoshi Shinozaki, Katsuhiko Nakata, Heather Sheardown

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Purpose. To review the development of artificial corneas (prostheses and tissue equivalents) for transplantation, and to provide recent updates on our tissue-engineered replacement corneas. Methods. Modified natural polymers and synthetic polymers were screened for their potential to replace damaged portions of the human cornea or the entire corneal thickness. These polymers, combined with cells derived from each of the three main corneal layers or stem cells, were used to develop artificial corneas. Functional testing was performed in vitro. Trials of biocompatibility and immune and inflammatory reactions were performed by implanting the most promising polymers into rabbit corneas. Results. Collagen-based biopolymers, combined with synthetic crosslinkers or copolymers, formed effective scaffolds for developing prototype artificial corneas that could be used as tissue replacements in the future. We have previously developed an artificial cornea that mimicked key morphologic and functional properties of the human cornea. The addition of synthetic polymers increased its toughness as it retained transparency and low light scattering, making the matrix scaffold more suitable for transplantation. These new composites were implanted into rabbits without causing any acute inflammation or immune response. We have also fabricated full-thickness composites that can be fully sutured. However, the long-term effects of these artificial corneas need to be evaluated. Conclusions. Novel tissue-engineered corneas that comprise composites of natural and synthetic biopolymers together with corneal cell lines or stem cells will, in the future, replace portions of the cornea that are damaged. Our results provide a basis for the development of both implantable temporary and permanent corneal replacements.

Original languageEnglish (US)
JournalCornea
Volume21
Issue number7 SUPPL.
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Corneal Transplantation
Cornea
Regeneration
Polymers
Biopolymers
Stem Cells
Rabbits
Tissue Transplantation
Prostheses and Implants
Collagen
Transplantation
Inflammation

Keywords

  • Artificial corneas
  • Keratoprostheses
  • Polymers
  • Tissue engineering
  • Tissue equivalents
  • Transplantation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Griffith, M., Hakim, M., Shimmura, S., Watsky, M. A., Li, F., Carlsson, D., ... Sheardown, H. (2002). Artificial human corneas: Scaffolds for transplantation and host regeneration. Cornea, 21(7 SUPPL.). https://doi.org/10.1097/01.ico.0000263120.68768.f8

Artificial human corneas : Scaffolds for transplantation and host regeneration. / Griffith, May; Hakim, Malik; Shimmura, Shigeto; Watsky, Mitchell Aaron; Li, Fengfu; Carlsson, David; Doillon, Charles J.; Nakamura, Masatsugu; Suuronen, Erik; Shinozaki, Naoshi; Nakata, Katsuhiko; Sheardown, Heather.

In: Cornea, Vol. 21, No. 7 SUPPL., 01.01.2002.

Research output: Contribution to journalArticle

Griffith, M, Hakim, M, Shimmura, S, Watsky, MA, Li, F, Carlsson, D, Doillon, CJ, Nakamura, M, Suuronen, E, Shinozaki, N, Nakata, K & Sheardown, H 2002, 'Artificial human corneas: Scaffolds for transplantation and host regeneration', Cornea, vol. 21, no. 7 SUPPL.. https://doi.org/10.1097/01.ico.0000263120.68768.f8
Griffith, May ; Hakim, Malik ; Shimmura, Shigeto ; Watsky, Mitchell Aaron ; Li, Fengfu ; Carlsson, David ; Doillon, Charles J. ; Nakamura, Masatsugu ; Suuronen, Erik ; Shinozaki, Naoshi ; Nakata, Katsuhiko ; Sheardown, Heather. / Artificial human corneas : Scaffolds for transplantation and host regeneration. In: Cornea. 2002 ; Vol. 21, No. 7 SUPPL.
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AU - Hakim, Malik

AU - Shimmura, Shigeto

AU - Watsky, Mitchell Aaron

AU - Li, Fengfu

AU - Carlsson, David

AU - Doillon, Charles J.

AU - Nakamura, Masatsugu

AU - Suuronen, Erik

AU - Shinozaki, Naoshi

AU - Nakata, Katsuhiko

AU - Sheardown, Heather

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N2 - Purpose. To review the development of artificial corneas (prostheses and tissue equivalents) for transplantation, and to provide recent updates on our tissue-engineered replacement corneas. Methods. Modified natural polymers and synthetic polymers were screened for their potential to replace damaged portions of the human cornea or the entire corneal thickness. These polymers, combined with cells derived from each of the three main corneal layers or stem cells, were used to develop artificial corneas. Functional testing was performed in vitro. Trials of biocompatibility and immune and inflammatory reactions were performed by implanting the most promising polymers into rabbit corneas. Results. Collagen-based biopolymers, combined with synthetic crosslinkers or copolymers, formed effective scaffolds for developing prototype artificial corneas that could be used as tissue replacements in the future. We have previously developed an artificial cornea that mimicked key morphologic and functional properties of the human cornea. The addition of synthetic polymers increased its toughness as it retained transparency and low light scattering, making the matrix scaffold more suitable for transplantation. These new composites were implanted into rabbits without causing any acute inflammation or immune response. We have also fabricated full-thickness composites that can be fully sutured. However, the long-term effects of these artificial corneas need to be evaluated. Conclusions. Novel tissue-engineered corneas that comprise composites of natural and synthetic biopolymers together with corneal cell lines or stem cells will, in the future, replace portions of the cornea that are damaged. Our results provide a basis for the development of both implantable temporary and permanent corneal replacements.

AB - Purpose. To review the development of artificial corneas (prostheses and tissue equivalents) for transplantation, and to provide recent updates on our tissue-engineered replacement corneas. Methods. Modified natural polymers and synthetic polymers were screened for their potential to replace damaged portions of the human cornea or the entire corneal thickness. These polymers, combined with cells derived from each of the three main corneal layers or stem cells, were used to develop artificial corneas. Functional testing was performed in vitro. Trials of biocompatibility and immune and inflammatory reactions were performed by implanting the most promising polymers into rabbit corneas. Results. Collagen-based biopolymers, combined with synthetic crosslinkers or copolymers, formed effective scaffolds for developing prototype artificial corneas that could be used as tissue replacements in the future. We have previously developed an artificial cornea that mimicked key morphologic and functional properties of the human cornea. The addition of synthetic polymers increased its toughness as it retained transparency and low light scattering, making the matrix scaffold more suitable for transplantation. These new composites were implanted into rabbits without causing any acute inflammation or immune response. We have also fabricated full-thickness composites that can be fully sutured. However, the long-term effects of these artificial corneas need to be evaluated. Conclusions. Novel tissue-engineered corneas that comprise composites of natural and synthetic biopolymers together with corneal cell lines or stem cells will, in the future, replace portions of the cornea that are damaged. Our results provide a basis for the development of both implantable temporary and permanent corneal replacements.

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KW - Tissue equivalents

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