Artificial human corneas: Scaffolds for transplantation and host regeneration

May Griffith, Malik Hakim, Shigeto Shimmura, Mitchell A. Watsky, Fengfu Li, David Carlsson, Charles J. Doillon, Masatsugu Nakamura, Erik Suuronen, Naoshi Shinozaki, Katsuhiko Nakata, Heather Sheardown

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Purpose. To review the development of artificial corneas (prostheses and tissue equivalents) for transplantation, and to provide recent updates on our tissue-engineered replacement corneas. Methods. Modified natural polymers and synthetic polymers were screened for their potential to replace damaged portions of the human cornea or the entire corneal thickness. These polymers, combined with cells derived from each of the three main corneal layers or stem cells, were used to develop artificial corneas. Functional testing was performed in vitro. Trials of biocompatibility and immune and inflammatory reactions were performed by implanting the most promising polymers into rabbit corneas. Results. Collagen-based biopolymers, combined with synthetic crosslinkers or copolymers, formed effective scaffolds for developing prototype artificial corneas that could be used as tissue replacements in the future. We have previously developed an artificial cornea that mimicked key morphologic and functional properties of the human cornea. The addition of synthetic polymers increased its toughness as it retained transparency and low light scattering, making the matrix scaffold more suitable for transplantation. These new composites were implanted into rabbits without causing any acute inflammation or immune response. We have also fabricated full-thickness composites that can be fully sutured. However, the long-term effects of these artificial corneas need to be evaluated. Conclusions. Novel tissue-engineered corneas that comprise composites of natural and synthetic biopolymers together with corneal cell lines or stem cells will, in the future, replace portions of the cornea that are damaged. Our results provide a basis for the development of both implantable temporary and permanent corneal replacements.

Original languageEnglish (US)
Pages (from-to)S54-S61
JournalCornea
Volume21
Issue number7 SUPPL.
DOIs
StatePublished - Oct 2002
Externally publishedYes

Keywords

  • Artificial corneas
  • Keratoprostheses
  • Polymers
  • Tissue engineering
  • Tissue equivalents
  • Transplantation

ASJC Scopus subject areas

  • Ophthalmology

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  • Cite this

    Griffith, M., Hakim, M., Shimmura, S., Watsky, M. A., Li, F., Carlsson, D., Doillon, C. J., Nakamura, M., Suuronen, E., Shinozaki, N., Nakata, K., & Sheardown, H. (2002). Artificial human corneas: Scaffolds for transplantation and host regeneration. Cornea, 21(7 SUPPL.), S54-S61. https://doi.org/10.1097/01.ico.0000263120.68768.f8