Assessment of chemical exchange in tryptophan-albumin solution through 19F multicomponent transverse relaxation dispersion analysis

Ping Chang Lin

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A number of NMR methods possess the capability of probing chemical exchange dynamics in solution. However, certain drawbacks limit the applications of these NMR approaches, particularly, to a complex system. Here, we propose a procedure that integrates the regularized nonnegative least squares (NNLS) analysis of multiexponential T2 relaxation into Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments to probe chemical exchange in a multicompartmental system. The proposed procedure was validated through analysis of 19F T2 relaxation data of 6-fluoro-DL-tryptophan in a two-compartment solution with and without bovine serum albumin. Given the regularized NNLS analysis of a T2 relaxation curve acquired, for example, at the CPMG frequency υ CPMG = 125, the nature of two distinct peaks in the associated T2 distribution spectrum indicated 6-fluoro-DL-tryptophan either retaining the free state, with geometric mean/∗multiplicative standard deviation (MSD) = 1851.2 ms/1.51, or undergoing free/albumin-bound interconversion, with geometric mean/∗MSD = 236.8 ms∗/1.54, in the two-compartment system. Quantities of the individual tryptophan species were accurately reflected by the associated T2 peak areas, with an interconversion state-to-free state ratio of 0.45 ± 0.11. Furthermore, the CPMG relaxation dispersion analysis estimated the exchange rate between the free and albumin-bound states in this fluorinated tryptophan analog and the corresponding dissociation constant of the fluorinated tryptophan-albumin complex in the chemical-exchanging, two-compartment system.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalJournal of Biomolecular NMR
Volume62
Issue number2
DOIs
StatePublished - Jun 4 2015

Keywords

  • Complex system
  • Kinetics
  • Multiexponential
  • Nonnegative least squares analysis
  • Protein-ligand interaction

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

Fingerprint Dive into the research topics of 'Assessment of chemical exchange in tryptophan-albumin solution through <sup>19</sup>F multicomponent transverse relaxation dispersion analysis'. Together they form a unique fingerprint.

  • Cite this