Assessment of dental fluorosis in Mmp20+/- mice

R. Sharma, C. E. Tye, A. Arun, D. MacDonald, A. Chatterjee, T. Abrazinski, E. T. Everett, Gary M. Whitford, J. D. Bartlett

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The molecular mechanisms that underlie dental fluorosis are poorly understood. The retention of enamel proteins hallmarking fluorotic enamel may result from impaired hydrolysis and/or removal of enamel proteins. Previous studies have suggested that partial inhibition of Mmp20 expression is involved in the etiology of dental fluorosis. Here we ask if mice expressing only one functional Mmp20 allele are more susceptible to fluorosis. We demonstrate that Mmp20+/- mice express approximately half the amount of MMP20 as do wild-type mice. The Mmp20 heterozygous mice have normal-appearing enamel, with Vickers microhardness values similar to those of wild-type control enamel. Therefore, reduced MMP20 expression is not solely responsible for dental fluorosis. With 50-ppm-fluoride (F-) treatment ad libitum, the Mmp20+/- mice had F- tissue levels similar to those of Mmp20+/+ mice. No significant difference in enamel hardness was observed between the F-treated heterozygous and wild-type mice. Interestingly, we did find a small but significant difference in quantitative fluorescence between these two groups, which may be attributable to slightly higher protein content in the Mmp20+/- mouse enamel. We conclude that MMP20 plays a nominal role in dental enamel fluorosis.

Original languageEnglish (US)
Pages (from-to)788-792
Number of pages5
JournalJournal of Dental Research
Volume90
Issue number6
DOIs
StatePublished - Jun 1 2011

Fingerprint

Dental Fluorosis
Dental Enamel
Proteins
Hardness
Fluorides
Hydrolysis
Fluorescence
Alleles

Keywords

  • enamel
  • fluoride
  • gene expression
  • protease/proteinase
  • protein expression

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Sharma, R., Tye, C. E., Arun, A., MacDonald, D., Chatterjee, A., Abrazinski, T., ... Bartlett, J. D. (2011). Assessment of dental fluorosis in Mmp20+/- mice. Journal of Dental Research, 90(6), 788-792. https://doi.org/10.1177/0022034511398868

Assessment of dental fluorosis in Mmp20+/- mice. / Sharma, R.; Tye, C. E.; Arun, A.; MacDonald, D.; Chatterjee, A.; Abrazinski, T.; Everett, E. T.; Whitford, Gary M.; Bartlett, J. D.

In: Journal of Dental Research, Vol. 90, No. 6, 01.06.2011, p. 788-792.

Research output: Contribution to journalArticle

Sharma, R, Tye, CE, Arun, A, MacDonald, D, Chatterjee, A, Abrazinski, T, Everett, ET, Whitford, GM & Bartlett, JD 2011, 'Assessment of dental fluorosis in Mmp20+/- mice', Journal of Dental Research, vol. 90, no. 6, pp. 788-792. https://doi.org/10.1177/0022034511398868
Sharma R, Tye CE, Arun A, MacDonald D, Chatterjee A, Abrazinski T et al. Assessment of dental fluorosis in Mmp20+/- mice. Journal of Dental Research. 2011 Jun 1;90(6):788-792. https://doi.org/10.1177/0022034511398868
Sharma, R. ; Tye, C. E. ; Arun, A. ; MacDonald, D. ; Chatterjee, A. ; Abrazinski, T. ; Everett, E. T. ; Whitford, Gary M. ; Bartlett, J. D. / Assessment of dental fluorosis in Mmp20+/- mice. In: Journal of Dental Research. 2011 ; Vol. 90, No. 6. pp. 788-792.
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