Assignment of 55 novel cosmids to seven subregions of chromosome 13 using fluorescence in situ hybridization

Lesleyann Hawthorn, Dean Nizetic, Hans Lehrach, John Kenneth Cowell

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Fifty-five individual cosmids isolated from a chromosome 13-specific library have been regionally assigned using fluorescence in situ hybridization. These cosmids represent novel DNA markers that can be added to the limited number of DNA probes already available for this chromosome. Individual cosmids mapped along the length of the chromosome with almost equal distribution except that there appears to be an excess of probes from the distal 13q33-q34 region. Only two cosmids did not map back to chromosome 13, indicating that at least 96% of cosmids in this library are from this chromosome. The library is generally available as gridded colonies on nylon membranes suitable for hybridization screening.

Original languageEnglish (US)
Pages (from-to)248-250
Number of pages3
JournalGenomics
Volume21
Issue number1
DOIs
StatePublished - May 1 1994
Externally publishedYes

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Chromosomes, Human, Pair 13
Cosmids
Fluorescence In Situ Hybridization
Libraries
Chromosomes
Nylons
DNA Probes
Genetic Markers
Membranes

ASJC Scopus subject areas

  • Genetics

Cite this

Assignment of 55 novel cosmids to seven subregions of chromosome 13 using fluorescence in situ hybridization. / Hawthorn, Lesleyann; Nizetic, Dean; Lehrach, Hans; Cowell, John Kenneth.

In: Genomics, Vol. 21, No. 1, 01.05.1994, p. 248-250.

Research output: Contribution to journalArticle

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