Assignment of the human folate transporter gene to chromosome 21q22.3 by somatic cell hybrid analysis and in situ hybridization

Teresa L. Yang-Feng, Yen Ying Ma, Rong Liang, Puttur D Prasad, Frederick H. Leibach, Vadivel Ganapathy

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have used the human folate transporter cDNA and a human genomic clone hybridizing to the cDNA to perform chromosomal mapping of the folate transporter gene. Human-rodent somatic cell hybrid analysis using the cDNA as the probe revealed perfect segregation with human chromosome 21. In situ hybridization of the cDNA probe to human metaphase chromosome spreads mapped the folate transporter gene to the distal long arm of chromosome 21, band q22.3. This chromosomal location was confirmed by fluorescence in situ hybridization using the genomic clone.

Original languageEnglish (US)
Pages (from-to)874-879
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume210
Issue number3
DOIs
StatePublished - May 25 1995

Fingerprint

Folic Acid Transporters
Hybrid Cells
Chromosomes
In Situ Hybridization
Complementary DNA
Genes
Chromosomes, Human, Pair 21
Human Chromosomes
Clone Cells
Metaphase
Fluorescence In Situ Hybridization
Rodentia
Fluorescence

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Assignment of the human folate transporter gene to chromosome 21q22.3 by somatic cell hybrid analysis and in situ hybridization. / Yang-Feng, Teresa L.; Ma, Yen Ying; Liang, Rong; Prasad, Puttur D; Leibach, Frederick H.; Ganapathy, Vadivel.

In: Biochemical and Biophysical Research Communications, Vol. 210, No. 3, 25.05.1995, p. 874-879.

Research output: Contribution to journalArticle

Yang-Feng, Teresa L. ; Ma, Yen Ying ; Liang, Rong ; Prasad, Puttur D ; Leibach, Frederick H. ; Ganapathy, Vadivel. / Assignment of the human folate transporter gene to chromosome 21q22.3 by somatic cell hybrid analysis and in situ hybridization. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 210, No. 3. pp. 874-879.
@article{b1cc0920dede4622aca48e53fc244b88,
title = "Assignment of the human folate transporter gene to chromosome 21q22.3 by somatic cell hybrid analysis and in situ hybridization",
abstract = "We have used the human folate transporter cDNA and a human genomic clone hybridizing to the cDNA to perform chromosomal mapping of the folate transporter gene. Human-rodent somatic cell hybrid analysis using the cDNA as the probe revealed perfect segregation with human chromosome 21. In situ hybridization of the cDNA probe to human metaphase chromosome spreads mapped the folate transporter gene to the distal long arm of chromosome 21, band q22.3. This chromosomal location was confirmed by fluorescence in situ hybridization using the genomic clone.",
author = "Yang-Feng, {Teresa L.} and Ma, {Yen Ying} and Rong Liang and Prasad, {Puttur D} and Leibach, {Frederick H.} and Vadivel Ganapathy",
year = "1995",
month = "5",
day = "25",
doi = "10.1006/bbrc.1995.1739",
language = "English (US)",
volume = "210",
pages = "874--879",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Assignment of the human folate transporter gene to chromosome 21q22.3 by somatic cell hybrid analysis and in situ hybridization

AU - Yang-Feng, Teresa L.

AU - Ma, Yen Ying

AU - Liang, Rong

AU - Prasad, Puttur D

AU - Leibach, Frederick H.

AU - Ganapathy, Vadivel

PY - 1995/5/25

Y1 - 1995/5/25

N2 - We have used the human folate transporter cDNA and a human genomic clone hybridizing to the cDNA to perform chromosomal mapping of the folate transporter gene. Human-rodent somatic cell hybrid analysis using the cDNA as the probe revealed perfect segregation with human chromosome 21. In situ hybridization of the cDNA probe to human metaphase chromosome spreads mapped the folate transporter gene to the distal long arm of chromosome 21, band q22.3. This chromosomal location was confirmed by fluorescence in situ hybridization using the genomic clone.

AB - We have used the human folate transporter cDNA and a human genomic clone hybridizing to the cDNA to perform chromosomal mapping of the folate transporter gene. Human-rodent somatic cell hybrid analysis using the cDNA as the probe revealed perfect segregation with human chromosome 21. In situ hybridization of the cDNA probe to human metaphase chromosome spreads mapped the folate transporter gene to the distal long arm of chromosome 21, band q22.3. This chromosomal location was confirmed by fluorescence in situ hybridization using the genomic clone.

UR - http://www.scopus.com/inward/record.url?scp=0029089831&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029089831&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.1739

DO - 10.1006/bbrc.1995.1739

M3 - Article

VL - 210

SP - 874

EP - 879

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -