Association between periodontitis and hyperlipidemia

Cause or effect?

Christopher W Cutler, Edward A. Shinedling, Martha Nunn, Ravi Jotwani, Byung Ock Kim, Salvador Nares, Anthony M. Iacopino

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Background: Epidemiological studies suggest a relationship between periodontitis and coronary artery disease, but the mechanism has not been established. Recent studies in animals indicate that low dose endotoxin, as in a Gram-negative infection, can induce hyperlipidemia and myeloid cell hyperactivity. The association between periodontitis, systemic exposure to Porphyromonas gingivalis, lipopolysaccharides (LPS), and hyperlipidemia has not been examined in humans. Methods: Sera were obtained from 26 adult periodontitis patients and 25 healthy control (C) subjects selected from patients and staff. Serum antibodies against Porphyromonas gingivalis and its LPS were analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting, respectively. Serum triglycerides (TG) and cholesterol (CHOL) were assayed by a commercial laboratory. The associations between AP and blood levels of TG, CHOL, and anti-P. gingivalis whole cells and LPS were examined by logistic regression analysis. Peripheral blood polymorphonuclear leukocytes (PMNs) from 6 healthy fasted donors were incubated with purified TG (0.1 mg/ml) for 2 hours at 37°C, stimulated with 100 ng/ml P. gingivalis LPS, and the release of IL-1β measured by ELISA. Results: The presence of periodontitis was significantly associated with age (odds ratio = 3.5, P = 0.04), elevated TG levels (odds ratio = 8.6, P = 0.0009), elevated CHOL levels (odds ratio = 7, P = 0.004), elevated ELISA titer (odds ratio = 35, P = 0.003) and reactivity with P. gingivalis LPS (odds ratio = 41, P = 0.001). PMNs from all 6 healthy patients released modest levels of IL-1β (10 to 60 pg/ml) when stimulated with 100 ng/ml P. gingivalis LPS. Addition of TG resulted in a significant increase (P<0.05) in IL-1β secreted that ranged from 7 to 150% over LPS alone. No IL-1β was elicited by TG or vehicle alone. Conclusions: The results of this study indicate the presence of a significant relationship between periodontitis, hyperlipidemia, and serum antibodies against P. gingivalis LPS that warrants further examination in a larger patient population. Furthermore, these studies indicate that elevated triglycerides are able to modulate IL-1β production by PMNs stimulated with P. gingivalis LPS. J Periodontol 1999;70:1429-1434.

Original languageEnglish (US)
Pages (from-to)1429-1434
Number of pages6
JournalJournal of Periodontology
Volume70
Issue number12
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

Periodontitis
Porphyromonas gingivalis
Hyperlipidemias
Lipopolysaccharides
Triglycerides
Interleukin-1
Odds Ratio
Enzyme-Linked Immunosorbent Assay
Serum
Cholesterol
Chronic Periodontitis
Antibodies
Myeloid Cells
Hypercholesterolemia
Endotoxins
Interleukin-10
Epidemiologic Studies
Coronary Artery Disease
Neutrophils
Logistic Models

Keywords

  • Antibodies, bacterial
  • Cholesterol
  • Cytokines
  • Hyperlipidemia
  • Lipopolysaccharides
  • Logistic regression analysis
  • Neutrophils
  • Periodontitis
  • Triglycerides

ASJC Scopus subject areas

  • Periodontics

Cite this

Cutler, C. W., Shinedling, E. A., Nunn, M., Jotwani, R., Kim, B. O., Nares, S., & Iacopino, A. M. (1999). Association between periodontitis and hyperlipidemia: Cause or effect? Journal of Periodontology, 70(12), 1429-1434. https://doi.org/10.1902/jop.1999.70.12.1429

Association between periodontitis and hyperlipidemia : Cause or effect? / Cutler, Christopher W; Shinedling, Edward A.; Nunn, Martha; Jotwani, Ravi; Kim, Byung Ock; Nares, Salvador; Iacopino, Anthony M.

In: Journal of Periodontology, Vol. 70, No. 12, 01.01.1999, p. 1429-1434.

Research output: Contribution to journalArticle

Cutler, CW, Shinedling, EA, Nunn, M, Jotwani, R, Kim, BO, Nares, S & Iacopino, AM 1999, 'Association between periodontitis and hyperlipidemia: Cause or effect?', Journal of Periodontology, vol. 70, no. 12, pp. 1429-1434. https://doi.org/10.1902/jop.1999.70.12.1429
Cutler, Christopher W ; Shinedling, Edward A. ; Nunn, Martha ; Jotwani, Ravi ; Kim, Byung Ock ; Nares, Salvador ; Iacopino, Anthony M. / Association between periodontitis and hyperlipidemia : Cause or effect?. In: Journal of Periodontology. 1999 ; Vol. 70, No. 12. pp. 1429-1434.
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AU - Shinedling, Edward A.

AU - Nunn, Martha

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AU - Kim, Byung Ock

AU - Nares, Salvador

AU - Iacopino, Anthony M.

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KW - Antibodies, bacterial

KW - Cholesterol

KW - Cytokines

KW - Hyperlipidemia

KW - Lipopolysaccharides

KW - Logistic regression analysis

KW - Neutrophils

KW - Periodontitis

KW - Triglycerides

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