Association of glycolipids and growth factor receptors in lipid rafts

Jing Wang, Robert K. Yu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The traditional methods to study lipid rafts and their association with membrane proteins are based mainly on the isolation of a detergent-resistant membrane by biochemical fractionation. However, the use of detergents may induce lipid segregation and/or redistribution of membrane proteins during the process of sample preparation. Here, we describe a detergent-free method to study the glycolipid and growth factor receptor interaction and their association with lipid rafts. This method combines the biochemical and immunoblotting tools with confocal microscopic imaging, which allows for evaluation and verification of the membrane protein interaction and association with the lipid rafts components in a multifaceted manner.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages131-145
Number of pages15
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2187
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Density gradient
  • Ganglioside
  • Growth factor receptor
  • Lipid raft
  • Neural stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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