Association of IGF-I and IGF-II with myofiber regeneration in vivo

Heidi L. Keller, Barbara St Pierre Schneider, Lois A. Eppihimer, Joseph Gerard Cannon

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

This study examined expression of insulinlike growth factor (IGF) in the myofibers and nonmyofibrillar structures of murine soleus muscle following contraction-induced damage. Identifying the cellular sources of this myogenic growth factor could improve muscle rehabilitation strategies. Immunohistochemical analysis of muscle sections indicated that the number of myofibers expressing both IGF-I and IGF-II increased significantly at 4, 7, and 10 days following injury, compared with control. Muscle spindles and vascular tissue expressed only IGF-II, and staining intensity did not change following injury. The number of fibers expressing developmental myosin heavy chain increased significantly at 7 and 10 days postinjury, and these usually coexpressed IGF. No IGF-specific staining of interstitial/inflammatory cells was observed. Therefore, expression of IGF after mechanically induced fiber damage occurs exclusively within regenerating fibers without supplemental delivery of IGF to the tissue by inflammatory cells or changes in constitutive expression of IGF-II in vascular tissue.

Original languageEnglish (US)
Pages (from-to)347-354
Number of pages8
JournalMuscle and Nerve
Volume22
Issue number3
DOIs
StatePublished - Mar 16 1999

Fingerprint

Regeneration
Intercellular Signaling Peptides and Proteins
Blood Vessels
Staining and Labeling
Muscle Spindles
Muscles
Myosin Heavy Chains
Wounds and Injuries
Muscle Contraction
Skeletal Muscle
Rehabilitation

Keywords

  • Damage
  • IGF
  • Immunohistochemistry
  • Inflammation
  • Necrosis

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

Association of IGF-I and IGF-II with myofiber regeneration in vivo. / Keller, Heidi L.; St Pierre Schneider, Barbara; Eppihimer, Lois A.; Cannon, Joseph Gerard.

In: Muscle and Nerve, Vol. 22, No. 3, 16.03.1999, p. 347-354.

Research output: Contribution to journalArticle

Keller, Heidi L. ; St Pierre Schneider, Barbara ; Eppihimer, Lois A. ; Cannon, Joseph Gerard. / Association of IGF-I and IGF-II with myofiber regeneration in vivo. In: Muscle and Nerve. 1999 ; Vol. 22, No. 3. pp. 347-354.
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