Association of IGF-I and IGF-II with myofiber regeneration in vivo

Heidi L. Keller, Barbara St Pierre Schneider, Lois A. Eppihimer, Joseph G. Cannon

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


This study examined expression of insulinlike growth factor (IGF) in the myofibers and nonmyofibrillar structures of murine soleus muscle following contraction-induced damage. Identifying the cellular sources of this myogenic growth factor could improve muscle rehabilitation strategies. Immunohistochemical analysis of muscle sections indicated that the number of myofibers expressing both IGF-I and IGF-II increased significantly at 4, 7, and 10 days following injury, compared with control. Muscle spindles and vascular tissue expressed only IGF-II, and staining intensity did not change following injury. The number of fibers expressing developmental myosin heavy chain increased significantly at 7 and 10 days postinjury, and these usually coexpressed IGF. No IGF-specific staining of interstitial/inflammatory cells was observed. Therefore, expression of IGF after mechanically induced fiber damage occurs exclusively within regenerating fibers without supplemental delivery of IGF to the tissue by inflammatory cells or changes in constitutive expression of IGF-II in vascular tissue.

Original languageEnglish (US)
Pages (from-to)347-354
Number of pages8
JournalMuscle and Nerve
Issue number3
StatePublished - 1999
Externally publishedYes


  • Damage
  • IGF
  • Immunohistochemistry
  • Inflammation
  • Necrosis

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)


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