Association of leptin gene polymorphism with economic traits in crossbred cattle

Vivek Choudhary, Pushpendra Kumar, Chinmoy Mishra, Tarun Kumar Bhattacharya, Bharat Bhushan, Arjava Sharma

Research output: Contribution to journalArticle

Abstract

The present investigation was carried out to identify two polymorphisms in leptin gene by PCR-RFLP and to examine the possible association of the identified genotypes with growth, production and reproduction traits in 205 female crossbred cattle (½ Holstein Friesian × ½ Hariana). One fragment of 330 bp comprising partial intron 2 and exon 3, and another fragment of 94 bp comprising partial exon 2 of leptin gene were amplified, and digested with HphI and Kpn2I restriction enzymes, respectively, for identification of genotypes. The animals with HphI-RFLPCT and Kpn2I-RFLP-AV genotypes had significantly higher birth weight than the HphI-RFLP-CC and Kpn2IRFLP-AA genotypes respectively. The HphI-RFLP locus had significant effect on body weight at 12 months of age, age at first calving and average daily milk yield, while, Kpn2I-RFLP had significant effect on first lactation milk yield and average daily milk yield. Therefore, leptin gene could be used as a marker for genetic selection of economic traits in cattle.

Original languageEnglish (US)
Pages (from-to)974-978
Number of pages5
JournalIndian Journal of Animal Sciences
Volume89
Issue number9
StatePublished - Jan 1 2019

Fingerprint

Leptin
leptin
Restriction Fragment Length Polymorphisms
crossbreds
restriction fragment length polymorphism
Economics
genetic polymorphism
economics
Genotype
cattle
milk yield
Milk
genotype
Genes
genes
exons
Exons
Genetic Selection
Lactation
Birth Weight

Keywords

  • Cattle
  • Crossbred
  • Economic traits
  • Gene
  • Leptin
  • PCR
  • Polymorphism

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Choudhary, V., Kumar, P., Mishra, C., Bhattacharya, T. K., Bhushan, B., & Sharma, A. (2019). Association of leptin gene polymorphism with economic traits in crossbred cattle. Indian Journal of Animal Sciences, 89(9), 974-978.

Association of leptin gene polymorphism with economic traits in crossbred cattle. / Choudhary, Vivek; Kumar, Pushpendra; Mishra, Chinmoy; Bhattacharya, Tarun Kumar; Bhushan, Bharat; Sharma, Arjava.

In: Indian Journal of Animal Sciences, Vol. 89, No. 9, 01.01.2019, p. 974-978.

Research output: Contribution to journalArticle

Choudhary, V, Kumar, P, Mishra, C, Bhattacharya, TK, Bhushan, B & Sharma, A 2019, 'Association of leptin gene polymorphism with economic traits in crossbred cattle', Indian Journal of Animal Sciences, vol. 89, no. 9, pp. 974-978.
Choudhary V, Kumar P, Mishra C, Bhattacharya TK, Bhushan B, Sharma A. Association of leptin gene polymorphism with economic traits in crossbred cattle. Indian Journal of Animal Sciences. 2019 Jan 1;89(9):974-978.
Choudhary, Vivek ; Kumar, Pushpendra ; Mishra, Chinmoy ; Bhattacharya, Tarun Kumar ; Bhushan, Bharat ; Sharma, Arjava. / Association of leptin gene polymorphism with economic traits in crossbred cattle. In: Indian Journal of Animal Sciences. 2019 ; Vol. 89, No. 9. pp. 974-978.
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