Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma

Paul M. Weinberger, Bao Ling Adam, Christine G. Gourin, William H. Moretz, Roni J. Bollag, Beverly Y. Wang, Zhongmin Liu, Jeffrey R. Lee, David J. Terris

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objectives: To characterize the localization of galectin-3 in benign and malignant thyroid neoplasms and to correlate this with alterations in β-catenin and cyclin D1 expression. Design: Immunohistochemical study of 116 paraffin-embedded archival specimens from 113 patients who had undergone thyroidectomy and tissue placed into a commercially available tissue microarray. Setting: Tertiary care hospital. Interventions: Thyroid tissue microarrays were stained by standard immunohistochemical protocols with monoclonal antibodies against galectin-3, β-catenin, and cyclin D1. Main Outcome Measures: Nuclear and cytoplasmic expression of galectin-3 was correlated with clinical parameters, β-catenin, and cyclin D1 expression. Results: Both cytoplasmic (56%) and nuclear (42%) galectin-3 expression was observed in most malignant neoplasms but was absent in benign thyroid specimens (P<.001). Among carcinomas, cytoplasmic galectin-3 expression was observed in papillary thyroid carcinomas (82%) and follicular (33%) and medullary (9%) carcinomas but was absent in anaplastic carcinomas (P<.001). Galectin-3 nuclear expression was observed in papillary thyroid carcinomas (62%) and follicular carcinomas (33%) but was undetectable in medullary, anaplastic carcinomas (P<.001). Cytoplasmic but not nuclear galectin-3 was inversely correlated with American Joint Committee on Cancer TNM stage (P=.02). There was a strong correlation between cytoplasmic and nuclear β-catenin expression and both nuclear (P=.04) and cytoplasmic (P=.003) galectin-3 expression. Similarly, there was a strong association between galectin-3 nuclear (P<.001) and cytoplasmic (P<.001) expression and cyclin D1 expression. Conclusion: Cytoplasmic and nuclear galectin-3 expression seem to be associated with activation of the Wnt-signaling pathway in well-differentiated thyroid neoplasms, suggesting that galectin-3 plays a role in thyroid carcinogenesis.

Original languageEnglish (US)
Pages (from-to)503-510
Number of pages8
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume133
Issue number5
DOIs
StatePublished - May 1 2007

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Galectin 3
Catenins
Wnt Signaling Pathway
Thyroid Neoplasms
Cyclin D1
Carcinoma
Thyroid Gland
Medullary Carcinoma
Thyroidectomy
Tertiary Healthcare
Tertiary Care Centers
Paraffin
Neoplasms
Carcinogenesis
Monoclonal Antibodies

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma. / Weinberger, Paul M.; Adam, Bao Ling; Gourin, Christine G.; Moretz, William H.; Bollag, Roni J.; Wang, Beverly Y.; Liu, Zhongmin; Lee, Jeffrey R.; Terris, David J.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 133, No. 5, 01.05.2007, p. 503-510.

Research output: Contribution to journalArticle

Weinberger, Paul M. ; Adam, Bao Ling ; Gourin, Christine G. ; Moretz, William H. ; Bollag, Roni J. ; Wang, Beverly Y. ; Liu, Zhongmin ; Lee, Jeffrey R. ; Terris, David J. / Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma. In: Archives of Otolaryngology - Head and Neck Surgery. 2007 ; Vol. 133, No. 5. pp. 503-510.
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title = "Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma",
abstract = "Objectives: To characterize the localization of galectin-3 in benign and malignant thyroid neoplasms and to correlate this with alterations in β-catenin and cyclin D1 expression. Design: Immunohistochemical study of 116 paraffin-embedded archival specimens from 113 patients who had undergone thyroidectomy and tissue placed into a commercially available tissue microarray. Setting: Tertiary care hospital. Interventions: Thyroid tissue microarrays were stained by standard immunohistochemical protocols with monoclonal antibodies against galectin-3, β-catenin, and cyclin D1. Main Outcome Measures: Nuclear and cytoplasmic expression of galectin-3 was correlated with clinical parameters, β-catenin, and cyclin D1 expression. Results: Both cytoplasmic (56{\%}) and nuclear (42{\%}) galectin-3 expression was observed in most malignant neoplasms but was absent in benign thyroid specimens (P<.001). Among carcinomas, cytoplasmic galectin-3 expression was observed in papillary thyroid carcinomas (82{\%}) and follicular (33{\%}) and medullary (9{\%}) carcinomas but was absent in anaplastic carcinomas (P<.001). Galectin-3 nuclear expression was observed in papillary thyroid carcinomas (62{\%}) and follicular carcinomas (33{\%}) but was undetectable in medullary, anaplastic carcinomas (P<.001). Cytoplasmic but not nuclear galectin-3 was inversely correlated with American Joint Committee on Cancer TNM stage (P=.02). There was a strong correlation between cytoplasmic and nuclear β-catenin expression and both nuclear (P=.04) and cytoplasmic (P=.003) galectin-3 expression. Similarly, there was a strong association between galectin-3 nuclear (P<.001) and cytoplasmic (P<.001) expression and cyclin D1 expression. Conclusion: Cytoplasmic and nuclear galectin-3 expression seem to be associated with activation of the Wnt-signaling pathway in well-differentiated thyroid neoplasms, suggesting that galectin-3 plays a role in thyroid carcinogenesis.",
author = "Weinberger, {Paul M.} and Adam, {Bao Ling} and Gourin, {Christine G.} and Moretz, {William H.} and Bollag, {Roni J.} and Wang, {Beverly Y.} and Zhongmin Liu and Lee, {Jeffrey R.} and Terris, {David J.}",
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T1 - Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma

AU - Weinberger, Paul M.

AU - Adam, Bao Ling

AU - Gourin, Christine G.

AU - Moretz, William H.

AU - Bollag, Roni J.

AU - Wang, Beverly Y.

AU - Liu, Zhongmin

AU - Lee, Jeffrey R.

AU - Terris, David J.

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Objectives: To characterize the localization of galectin-3 in benign and malignant thyroid neoplasms and to correlate this with alterations in β-catenin and cyclin D1 expression. Design: Immunohistochemical study of 116 paraffin-embedded archival specimens from 113 patients who had undergone thyroidectomy and tissue placed into a commercially available tissue microarray. Setting: Tertiary care hospital. Interventions: Thyroid tissue microarrays were stained by standard immunohistochemical protocols with monoclonal antibodies against galectin-3, β-catenin, and cyclin D1. Main Outcome Measures: Nuclear and cytoplasmic expression of galectin-3 was correlated with clinical parameters, β-catenin, and cyclin D1 expression. Results: Both cytoplasmic (56%) and nuclear (42%) galectin-3 expression was observed in most malignant neoplasms but was absent in benign thyroid specimens (P<.001). Among carcinomas, cytoplasmic galectin-3 expression was observed in papillary thyroid carcinomas (82%) and follicular (33%) and medullary (9%) carcinomas but was absent in anaplastic carcinomas (P<.001). Galectin-3 nuclear expression was observed in papillary thyroid carcinomas (62%) and follicular carcinomas (33%) but was undetectable in medullary, anaplastic carcinomas (P<.001). Cytoplasmic but not nuclear galectin-3 was inversely correlated with American Joint Committee on Cancer TNM stage (P=.02). There was a strong correlation between cytoplasmic and nuclear β-catenin expression and both nuclear (P=.04) and cytoplasmic (P=.003) galectin-3 expression. Similarly, there was a strong association between galectin-3 nuclear (P<.001) and cytoplasmic (P<.001) expression and cyclin D1 expression. Conclusion: Cytoplasmic and nuclear galectin-3 expression seem to be associated with activation of the Wnt-signaling pathway in well-differentiated thyroid neoplasms, suggesting that galectin-3 plays a role in thyroid carcinogenesis.

AB - Objectives: To characterize the localization of galectin-3 in benign and malignant thyroid neoplasms and to correlate this with alterations in β-catenin and cyclin D1 expression. Design: Immunohistochemical study of 116 paraffin-embedded archival specimens from 113 patients who had undergone thyroidectomy and tissue placed into a commercially available tissue microarray. Setting: Tertiary care hospital. Interventions: Thyroid tissue microarrays were stained by standard immunohistochemical protocols with monoclonal antibodies against galectin-3, β-catenin, and cyclin D1. Main Outcome Measures: Nuclear and cytoplasmic expression of galectin-3 was correlated with clinical parameters, β-catenin, and cyclin D1 expression. Results: Both cytoplasmic (56%) and nuclear (42%) galectin-3 expression was observed in most malignant neoplasms but was absent in benign thyroid specimens (P<.001). Among carcinomas, cytoplasmic galectin-3 expression was observed in papillary thyroid carcinomas (82%) and follicular (33%) and medullary (9%) carcinomas but was absent in anaplastic carcinomas (P<.001). Galectin-3 nuclear expression was observed in papillary thyroid carcinomas (62%) and follicular carcinomas (33%) but was undetectable in medullary, anaplastic carcinomas (P<.001). Cytoplasmic but not nuclear galectin-3 was inversely correlated with American Joint Committee on Cancer TNM stage (P=.02). There was a strong correlation between cytoplasmic and nuclear β-catenin expression and both nuclear (P=.04) and cytoplasmic (P=.003) galectin-3 expression. Similarly, there was a strong association between galectin-3 nuclear (P<.001) and cytoplasmic (P<.001) expression and cyclin D1 expression. Conclusion: Cytoplasmic and nuclear galectin-3 expression seem to be associated with activation of the Wnt-signaling pathway in well-differentiated thyroid neoplasms, suggesting that galectin-3 plays a role in thyroid carcinogenesis.

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