Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma

Paul Maurice Weinberger, Bao Ling Adam, Christine G. Gourin, William H. Moretz, Roni Jacob Bollag, Beverly Y. Wang, Zhongmin Liu, Jeffrey R Lee, David J Terris

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objectives: To characterize the localization of galectin-3 in benign and malignant thyroid neoplasms and to correlate this with alterations in β-catenin and cyclin D1 expression. Design: Immunohistochemical study of 116 paraffin-embedded archival specimens from 113 patients who had undergone thyroidectomy and tissue placed into a commercially available tissue microarray. Setting: Tertiary care hospital. Interventions: Thyroid tissue microarrays were stained by standard immunohistochemical protocols with monoclonal antibodies against galectin-3, β-catenin, and cyclin D1. Main Outcome Measures: Nuclear and cytoplasmic expression of galectin-3 was correlated with clinical parameters, β-catenin, and cyclin D1 expression. Results: Both cytoplasmic (56%) and nuclear (42%) galectin-3 expression was observed in most malignant neoplasms but was absent in benign thyroid specimens (P<.001). Among carcinomas, cytoplasmic galectin-3 expression was observed in papillary thyroid carcinomas (82%) and follicular (33%) and medullary (9%) carcinomas but was absent in anaplastic carcinomas (P<.001). Galectin-3 nuclear expression was observed in papillary thyroid carcinomas (62%) and follicular carcinomas (33%) but was undetectable in medullary, anaplastic carcinomas (P<.001). Cytoplasmic but not nuclear galectin-3 was inversely correlated with American Joint Committee on Cancer TNM stage (P=.02). There was a strong correlation between cytoplasmic and nuclear β-catenin expression and both nuclear (P=.04) and cytoplasmic (P=.003) galectin-3 expression. Similarly, there was a strong association between galectin-3 nuclear (P<.001) and cytoplasmic (P<.001) expression and cyclin D1 expression. Conclusion: Cytoplasmic and nuclear galectin-3 expression seem to be associated with activation of the Wnt-signaling pathway in well-differentiated thyroid neoplasms, suggesting that galectin-3 plays a role in thyroid carcinogenesis.

Original languageEnglish (US)
Pages (from-to)503-510
Number of pages8
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume133
Issue number5
DOIs
StatePublished - May 1 2007

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Galectin 3
Catenins
Wnt Signaling Pathway
Thyroid Neoplasms
Cyclin D1
Carcinoma
Thyroid Gland
Medullary Carcinoma
Thyroidectomy
Tertiary Healthcare
Tertiary Care Centers
Paraffin
Neoplasms
Carcinogenesis
Monoclonal Antibodies

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma. / Weinberger, Paul Maurice; Adam, Bao Ling; Gourin, Christine G.; Moretz, William H.; Bollag, Roni Jacob; Wang, Beverly Y.; Liu, Zhongmin; Lee, Jeffrey R; Terris, David J.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 133, No. 5, 01.05.2007, p. 503-510.

Research output: Contribution to journalArticle

Weinberger, Paul Maurice ; Adam, Bao Ling ; Gourin, Christine G. ; Moretz, William H. ; Bollag, Roni Jacob ; Wang, Beverly Y. ; Liu, Zhongmin ; Lee, Jeffrey R ; Terris, David J. / Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma. In: Archives of Otolaryngology - Head and Neck Surgery. 2007 ; Vol. 133, No. 5. pp. 503-510.
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title = "Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma",
abstract = "Objectives: To characterize the localization of galectin-3 in benign and malignant thyroid neoplasms and to correlate this with alterations in β-catenin and cyclin D1 expression. Design: Immunohistochemical study of 116 paraffin-embedded archival specimens from 113 patients who had undergone thyroidectomy and tissue placed into a commercially available tissue microarray. Setting: Tertiary care hospital. Interventions: Thyroid tissue microarrays were stained by standard immunohistochemical protocols with monoclonal antibodies against galectin-3, β-catenin, and cyclin D1. Main Outcome Measures: Nuclear and cytoplasmic expression of galectin-3 was correlated with clinical parameters, β-catenin, and cyclin D1 expression. Results: Both cytoplasmic (56{\%}) and nuclear (42{\%}) galectin-3 expression was observed in most malignant neoplasms but was absent in benign thyroid specimens (P<.001). Among carcinomas, cytoplasmic galectin-3 expression was observed in papillary thyroid carcinomas (82{\%}) and follicular (33{\%}) and medullary (9{\%}) carcinomas but was absent in anaplastic carcinomas (P<.001). Galectin-3 nuclear expression was observed in papillary thyroid carcinomas (62{\%}) and follicular carcinomas (33{\%}) but was undetectable in medullary, anaplastic carcinomas (P<.001). Cytoplasmic but not nuclear galectin-3 was inversely correlated with American Joint Committee on Cancer TNM stage (P=.02). There was a strong correlation between cytoplasmic and nuclear β-catenin expression and both nuclear (P=.04) and cytoplasmic (P=.003) galectin-3 expression. Similarly, there was a strong association between galectin-3 nuclear (P<.001) and cytoplasmic (P<.001) expression and cyclin D1 expression. Conclusion: Cytoplasmic and nuclear galectin-3 expression seem to be associated with activation of the Wnt-signaling pathway in well-differentiated thyroid neoplasms, suggesting that galectin-3 plays a role in thyroid carcinogenesis.",
author = "Weinberger, {Paul Maurice} and Adam, {Bao Ling} and Gourin, {Christine G.} and Moretz, {William H.} and Bollag, {Roni Jacob} and Wang, {Beverly Y.} and Zhongmin Liu and Lee, {Jeffrey R} and Terris, {David J}",
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T1 - Association of nuclear, cytoplasmic expression of galectin-3 with β-catenin/Wnt-pathway activation in thyroid carcinoma

AU - Weinberger, Paul Maurice

AU - Adam, Bao Ling

AU - Gourin, Christine G.

AU - Moretz, William H.

AU - Bollag, Roni Jacob

AU - Wang, Beverly Y.

AU - Liu, Zhongmin

AU - Lee, Jeffrey R

AU - Terris, David J

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Objectives: To characterize the localization of galectin-3 in benign and malignant thyroid neoplasms and to correlate this with alterations in β-catenin and cyclin D1 expression. Design: Immunohistochemical study of 116 paraffin-embedded archival specimens from 113 patients who had undergone thyroidectomy and tissue placed into a commercially available tissue microarray. Setting: Tertiary care hospital. Interventions: Thyroid tissue microarrays were stained by standard immunohistochemical protocols with monoclonal antibodies against galectin-3, β-catenin, and cyclin D1. Main Outcome Measures: Nuclear and cytoplasmic expression of galectin-3 was correlated with clinical parameters, β-catenin, and cyclin D1 expression. Results: Both cytoplasmic (56%) and nuclear (42%) galectin-3 expression was observed in most malignant neoplasms but was absent in benign thyroid specimens (P<.001). Among carcinomas, cytoplasmic galectin-3 expression was observed in papillary thyroid carcinomas (82%) and follicular (33%) and medullary (9%) carcinomas but was absent in anaplastic carcinomas (P<.001). Galectin-3 nuclear expression was observed in papillary thyroid carcinomas (62%) and follicular carcinomas (33%) but was undetectable in medullary, anaplastic carcinomas (P<.001). Cytoplasmic but not nuclear galectin-3 was inversely correlated with American Joint Committee on Cancer TNM stage (P=.02). There was a strong correlation between cytoplasmic and nuclear β-catenin expression and both nuclear (P=.04) and cytoplasmic (P=.003) galectin-3 expression. Similarly, there was a strong association between galectin-3 nuclear (P<.001) and cytoplasmic (P<.001) expression and cyclin D1 expression. Conclusion: Cytoplasmic and nuclear galectin-3 expression seem to be associated with activation of the Wnt-signaling pathway in well-differentiated thyroid neoplasms, suggesting that galectin-3 plays a role in thyroid carcinogenesis.

AB - Objectives: To characterize the localization of galectin-3 in benign and malignant thyroid neoplasms and to correlate this with alterations in β-catenin and cyclin D1 expression. Design: Immunohistochemical study of 116 paraffin-embedded archival specimens from 113 patients who had undergone thyroidectomy and tissue placed into a commercially available tissue microarray. Setting: Tertiary care hospital. Interventions: Thyroid tissue microarrays were stained by standard immunohistochemical protocols with monoclonal antibodies against galectin-3, β-catenin, and cyclin D1. Main Outcome Measures: Nuclear and cytoplasmic expression of galectin-3 was correlated with clinical parameters, β-catenin, and cyclin D1 expression. Results: Both cytoplasmic (56%) and nuclear (42%) galectin-3 expression was observed in most malignant neoplasms but was absent in benign thyroid specimens (P<.001). Among carcinomas, cytoplasmic galectin-3 expression was observed in papillary thyroid carcinomas (82%) and follicular (33%) and medullary (9%) carcinomas but was absent in anaplastic carcinomas (P<.001). Galectin-3 nuclear expression was observed in papillary thyroid carcinomas (62%) and follicular carcinomas (33%) but was undetectable in medullary, anaplastic carcinomas (P<.001). Cytoplasmic but not nuclear galectin-3 was inversely correlated with American Joint Committee on Cancer TNM stage (P=.02). There was a strong correlation between cytoplasmic and nuclear β-catenin expression and both nuclear (P=.04) and cytoplasmic (P=.003) galectin-3 expression. Similarly, there was a strong association between galectin-3 nuclear (P<.001) and cytoplasmic (P<.001) expression and cyclin D1 expression. Conclusion: Cytoplasmic and nuclear galectin-3 expression seem to be associated with activation of the Wnt-signaling pathway in well-differentiated thyroid neoplasms, suggesting that galectin-3 plays a role in thyroid carcinogenesis.

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