Abstract
The polyoma virus medium and small tumor antigens, as well as simian virus 40 small tumor antigen, form specific complexes with two cellular proteins designated 61- and 37-kDa proteins. In this report, we demonstrate that the 61-and 37-kDa proteins correspond to the A and C subunits, respectively, of the serine- and threonine-specific protein phosphatase 2A (PP2A). On the one hand, antibodies raised against the 61-kDa protein reacted specifically with the purified A subunit of PP2A. Furthermore, the amino acid sequences of seven tryptic peptides from the A subunit were almost identical to sequences of the 61-kDa protein as deduced from the corresponding cDNA sequence. On the other hand, antibodies against the purified C subunit (catalytic subunit) of PP2A reacted specifically with the medium tumor antigen-associated 37-kDa protein. These data suggest a role of PP2A in cell transformation by polyoma virus and simian virus 40.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2521-2525 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 87 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jan 1 1990 |
| Externally published | Yes |
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Keywords
- Antibodies against protein phosphatase 2A subunits
- Cell transformation
- Protein sequence
- Simian virus 40 small tumor antigen
ASJC Scopus subject areas
- General
Cite this
Association of protein phosphatase 2A with polyoma virus medium tumor antigen. / Walter, Gernot; Ruediger, Ralf; Slaughter, Clive; Mumby, Marc.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 7, 01.01.1990, p. 2521-2525.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of protein phosphatase 2A with polyoma virus medium tumor antigen
AU - Walter, Gernot
AU - Ruediger, Ralf
AU - Slaughter, Clive
AU - Mumby, Marc
PY - 1990/1/1
Y1 - 1990/1/1
N2 - The polyoma virus medium and small tumor antigens, as well as simian virus 40 small tumor antigen, form specific complexes with two cellular proteins designated 61- and 37-kDa proteins. In this report, we demonstrate that the 61-and 37-kDa proteins correspond to the A and C subunits, respectively, of the serine- and threonine-specific protein phosphatase 2A (PP2A). On the one hand, antibodies raised against the 61-kDa protein reacted specifically with the purified A subunit of PP2A. Furthermore, the amino acid sequences of seven tryptic peptides from the A subunit were almost identical to sequences of the 61-kDa protein as deduced from the corresponding cDNA sequence. On the other hand, antibodies against the purified C subunit (catalytic subunit) of PP2A reacted specifically with the medium tumor antigen-associated 37-kDa protein. These data suggest a role of PP2A in cell transformation by polyoma virus and simian virus 40.
AB - The polyoma virus medium and small tumor antigens, as well as simian virus 40 small tumor antigen, form specific complexes with two cellular proteins designated 61- and 37-kDa proteins. In this report, we demonstrate that the 61-and 37-kDa proteins correspond to the A and C subunits, respectively, of the serine- and threonine-specific protein phosphatase 2A (PP2A). On the one hand, antibodies raised against the 61-kDa protein reacted specifically with the purified A subunit of PP2A. Furthermore, the amino acid sequences of seven tryptic peptides from the A subunit were almost identical to sequences of the 61-kDa protein as deduced from the corresponding cDNA sequence. On the other hand, antibodies against the purified C subunit (catalytic subunit) of PP2A reacted specifically with the medium tumor antigen-associated 37-kDa protein. These data suggest a role of PP2A in cell transformation by polyoma virus and simian virus 40.
KW - Antibodies against protein phosphatase 2A subunits
KW - Cell transformation
KW - Protein sequence
KW - Simian virus 40 small tumor antigen
UR - http://www.scopus.com/inward/record.url?scp=0025366151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025366151&partnerID=8YFLogxK
U2 - 10.1073/pnas.87.7.2521
DO - 10.1073/pnas.87.7.2521
M3 - Article
C2 - 2157202
AN - SCOPUS:0025366151
VL - 87
SP - 2521
EP - 2525
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 7
ER -